Martin Moche

I oversee the Macromolecular X-ray crystallography activities at Protein Science Facility, Karolinska Institutet
I am the Macromolecular X-ray Crystallography (MX) manager at Protein Science Facility [1], Karolinska Institutet in daily use by around 15 researchers. I am organizing the local structural biology community to share synchrotron beamtime, crystallization robot hardware and remotely accessible diffraction data analysis software to achieve common goals and stay competitive in an international environment.
I am coordinating three synchrotron “block allocation groups” at Diamond Light Source in Oxfordshire and MAX IV in Lund in benefit of seventeen research groups at KI, SU, UU, KTH and LiU resulting in shared beamtime for Macromolecular X-ray diffraction experiments.

I am also heading a team developing a High Performance Computing integrated structural biology software installation at NSC Tetralith, NSC Berzelius, LUNARC Cosmos and the MAX IV cluster called PReSTO [2], remotely accessible from any pc, mac or Linux computer with internet access. 

2003-2005 postdoc with prof. Pär Nordlund at Stockholm University
1997-2003 Ph.D. studies with prof. Ylva Lindqvist at Karolinska Institutet
1991-1997 M.Sc. degree in Molecular Biophysics at Umeå University

[1] https://ki.se/en/mbb/psf-mx

[2] https://www.nsc.liu.se/support/presto/
[3] https://openarchive.ki.se/xmlui/handle/10616/38577

I am happy to collaborate with researchers that want to apply macromolecular X-ray crystallography to their research (1-5). I was the assistant supervisor of Madhanagopal Anandapadamanaban (6-8) that defended his thesis in December 2015.

1. Sandalova T, Sala BM, Moche M, Ljunggren H-G, Alici E, Henriques-Normark B, Agback T, Lesovoy D, Agback P, Achour A. Crystallographic and NMR studies of Streptococcus pneumonia LCP protein PsrSp indicate the importance of dynamics in four long loops for ligand specificity. bioRxiv. 2024:2024.10.21.619401:10.1101/2024.10.21.619401.

2. Jungholm O, Trkulja C, Moche M, Srinivasa SP, Christakopoulou MN, Davidson M, Reymer A, Jardemark K, Fogaca RL, Ashok A, Jeffries G, Ampah-Korsah H, Strandback E, Andrell J, Nyman T, Nouairia G, Orwar O. Novel druggable space in human KRAS G13D discovered using structural bioinformatics and a P-loop targeting monoclonal antibody. Sci Rep. 2024, 14(1):19656:10.1038/s41598-024-70217-9.

3. Akaberi D, Pourghasemi Lati M, Krambrich J, Berger J, Neilsen G, Strandback E, Turunen SP, Wannberg J, Gullberg H, Moche M, Chinthakindi PK, Nyman T, Sarafianos SG, Sandstrom A, Jarhult JD, Sandberg K, Lundkvist A, Verho O, Lennerstrand J. Identification of novel and potent inhibitors of SARS-CoV-2 main protease from DNA-encoded chemical libraries. Antimicrob Agents Chemother. 2024:e0090924:10.1128/aac.00909-24.

4. Combined x-ray crystallography and computational modeling approach to investigate the Hsp90 C-terminal peptide binding to FKBP51. Kumar R, Moche M, Winblad B, Pavlov PF. Sci Rep 2017 10, 7(1):14288

5. Enzymatic production of 'monoclonal stoichiometric' single-stranded DNA oligonucleotides. Ducani C, Kaul C, Moche M, Shih WM, Högberg B. Nat Methods 2013 Jul, 10(7):647-52

6. High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation. Anandapadamanaban M, Andresen C, Helander S, Ohyama Y, Siponen MI, Lundström P, Kokubo T, Ikura M, Moche M, Sunnerhagen M Nat Struct Mol Biol 2013 Aug, 20(8):1008-14.

7. Mutation-Induced Population Shift in the MexR Conformational Ensemble Disengages DNA Binding: A Novel Mechanism for MarR Family Derepression. Anandapadamanaban M, Pilstål R, Andresen C, Trewhella J, Moche M, Wallner B, Sunnerhagen M. Structure 2016 08, 24(8):1311-1321

8. E3 ubiquitin-protein ligase TRIM21-mediated lysine capture by UBE2E1 reveals substrate-targeting mode of a ubiquitin-conjugating E2. Anandapadamanaban M, Kyriakidis NC, Csizmók V, Wallenhammar A, Espinosa AC, Ahlner A, Round AR, Trewhella J, Moche M, Wahren-Herlenius M, Sunnerhagen M J Biol Chem 2019 07, 294(30):11404-11419

I am usually teaching first and second year medical and biomedical students in fundamental concepts regarding protein, lipid and carbohydrate structure and function vitamins and cofactors energy metabolism such as glycolysis, TCA cycle and oxidative phosphorylation and impact of diabetes on insulin/glucagon regulation on gluconeogenesis, glycogenolysis and glycogen synthesis. I am also teaching about chemical bonding in organic chemistry.