2016: Associate Professor in Toxicology at the Institute of Environmental Medicine (IMM)
2006: Dr.Med.Sci., Karolinska Institutet. Scientific field: Medicine/ Environmental medicine. Thesis title: Particularly Harmful Particles? - A study of airborne particles with a focus on genotoxicity and oxidative stress.
2001: M.Sci. Chemistry from Karlstad University. Main subjects: Chemistry (Karlstad University), Environmental Sciences (Karlstad University), Human Biosciences/Biology (University of Newcastle, Australia) Toxicology (Karolinska Institutet)
Toxicity of particles with a focus on nanotoxicology
The introduction of nanotechnology has led to an increased likelihood of exposure to nanoparticles for workers and consumers. In addition, workers in occupations not related to nanotechnology are also frequently exposed to elevated levels of airborne metallic particles and ambient particles in cities often contain metals. My research is focused on understanding lung cell toxicity of metal-containing particles and one main aim is to find out which particles that are most toxic as well as understanding the underlying mechanisms. Specifically, there is a focus on the ability of particles to cause DNA damage and oxidative stress. A challenge with my research is to develop and use in vitro methods that better resembles the real exposure situation in the lung. This includes e.g. to expose lung cells to an aerosol of particles in the “air-liquid interface”. Oxidative stress and DNA damage arising from the particles can lead to mutations that are critical for cancer development. Furthermore, it has become clear that many toxic agents including metals can affect the DNA in other ways. Epigenetics is the study of heritable changes in gene expression or cellular phenotype caused by mechanisms other than changes in the underlying DNA sequence. On-going research also aims at studying epigenetic effects as a consequence of nanoparticle exposure. In all, these projects will be important for hazard identification and risk assessment of nanomaterials with the overall goal to prevent negative health effects following exposure to nanoparticles.
Selected recent publications
Gliga AR, Skoglund, S, Odnevall Wallinder, I, Fadeel, B and Karlsson HL (2014). Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release. Particle and Fibre Toxicology. Feb 17;11(1):11.
Karlsson HL, Cronholm P, Hedberg Y, Tornberg M, De Battice L, Svedhem S and Odnevall Wallinder I. (2013). Cell membrane damage and protein interaction induced by copper containing nanoparticles - importance of the metal release process. Toxicology, 8;313(1):59-69
Cronholm P, Karlsson HL, Hedberg J, Lowe TA, Winnberg L, Elihn K, Wallinder IO, Möller L. (2013). Intracellular uptake and toxicity of Ag and CuO Nanoparticles: A Comparison Between Nanoparticles and their Corresponding Metal Ions. Small. Apr 8;9(7):970-82.
Stoccoro A, Karlsson HL, Coppedè F, Migliore L. (2013). Epigenetic effects of nano-sized materials. Toxicology. 313(1), 3-14.
Elihn K, Cronholm P, Karlsson HL, Midander K, Odnevall Wallinder I and Möller, L (2013). Cellular doses of partly soluble Cu particle aerosols at the air-liquid interface using an artificial lung system. J Aerosol Med Pulm Drug Deliv. Apr;26(2):84-93.
Shi J, Karlsson HL, Johansson K, Gogvadze V, Xiao L, Li J, Burks T, Uheida A, Garcia-Bennett A, Muhammed M, Mathur S, Morgenstern R, Kagan V, Fadeel B. (2012). Microsomal Glutathione Transferase 1 Protects against Toxicity Induced by Silica Nanoparticles but not by Zinc Oxide Nanoparticles. ASC Nano, 6(3):1925-38.
Karlsson HL (2010). The comet assay in nanotoxicology research. Anal Bioanal Chem. 398(2):651-66.
Academic honours, awards and prizes
Receiver of stipend from ”Konung Carl XVI Gustafs 50-årsfond för vetenskap, teknik och miljö”, 2012
FORTE. Project title: “Nanoparticle-induced toxicity: focus on genotoxic and epigenetic effects in lung cells” (2012-2015)
Karolinska Institutet KID funding: “Nanoparticle-induced toxicity: focus on genotoxic and epigenetic effects in lung cells” (2012-2015)
IMM Junior Faculty Grant (2013-2014)
VR. Project title: “Nano-Cell interactions: DNA damage of well characterized metal and metal oxide nanoparticles investigated by using high-throughput comet assay and reporter cell lines” (2015-2018)
Electrochemical surface oxide characteristics of metal nanoparticles (Mn, Cu and Al) and the relation to toxicity
ELECTROCHIMICA ACTA 2016;:360-371
In vivo micronucleus screening in zebrafish by flow cytometry
Nickel Release, ROS Generation and Toxicity of Ni and NiO Micro- and Nanoparticles
PloS one 2016;11(7):e0159684-
Optimization of an air-liquid interface exposure system for assessing toxicity of airborne nanoparticles
Journal of applied toxicology : JAT 2016;36(10):1294-301
Surface passivity largely governs the bioaccessibility of nickel-based powder particles at human exposure conditions
Regulatory toxicology and pharmacology : RTP 2016;81():162-170
The importance of extracellular speciation and corrosion of copper nanoparticles on lung cell membrane integrity
Colloids and surfaces. B, Biointerfaces 2016;141():291-300
Can the comet assay be used reliably to detect nanoparticle-induced genotoxicity?
Environmental and molecular mutagenesis 2015;56(2):82-96
Mechanism-based genotoxicity screening of metal oxide nanoparticles using the ToxTracker panel of reporter cell lines
Particle and fibre toxicology 2014;11():41-
Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release
Particle and fibre toxicology 2014;11():11-
Cellular dose of partly soluble Cu particle aerosols at the air-liquid interface using an in vitro lung cell exposure system
Journal of aerosol medicine and pulmonary drug delivery 2013;26(2):84-93
Epigenetic effects of nano-sized materials
Geoengineering: Perilous Particles
Intracellular uptake and toxicity of Ag and CuO nanoparticles: a comparison between nanoparticles and their corresponding metal ions
Small (Weinheim an der Bergstrasse, Germany) 2013;9(7):970-82
Hemolytic properties of synthetic nano- and porous silica particles: the effect of surface properties and the protection by the plasma corona
Acta biomaterialia 2012;8(9):3478-90
Bioaccessibility, bioavailability and toxicity of commercially relevant iron- and chromium-based particles: in vitro studies with an inhalation perspective
PARTICLE AND FIBRE TOXICOLOGY 2010;:23-
The comet assay in nanotoxicology research
ANALYTICAL AND BIOANALYTICAL CHEMISTRY 2010;398(2):651-66
Size-dependent toxicity of metal oxide particles-A comparison between nano- and micrometer size
TOXICOLOGY LETTERS 2009;188(2):112-8
Copper oxide nanoparticles are highly toxic: A comparison between metal oxide nanoparticles and carbon nanotubes
CHEMICAL RESEARCH IN TOXICOLOGY 2008;21(9):1726-32
Mechanisms related to the genotoxicity of particles in the subway and from other sources
CHEMICAL RESEARCH IN TOXICOLOGY 2008;21(3):726-31
The antipsychotic drug trifluoperazine inhibits DNA repair and sensitizes non-small cell lung carcinoma cells to DNA double-strand break-induced cell death
MOLECULAR CANCER THERAPEUTICS 2007;6(8):2303-9
Comparison of genotoxic and inflammatory effects of particles generated by wood combustion, a road simulator and collected from street and subway
TOXICOLOGY LETTERS 2006;165(3):203-11
DNA oxidative damage and strand breaks in young healthy individuals: A gender difference and the role of life style factors
FREE RADICAL RESEARCH 2006;40(7):707-14
Establishing the background level of base oxidation in human lymphocyte DNA: results of an interlaboratory validation study
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2005;19(1):82-4
Subway particles are more genotoxic than street particles and induce oxidative stress in cultured human lung cells
CHEMICAL RESEARCH IN TOXICOLOGY 2005;18(1):19-23
Genotoxicity of airborne particulate matter: the role of cell-particle interaction and of substances with adduct-forming and oxidizing capacity
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 2004;565(1):1-10
P-32-postlabeling of DNA adducts arising from complex mixtures: HPLC versus TLC separation applied to adducts from petroleum products
ARCHIVES OF TOXICOLOGY 2004;78(3):174-81