LCI teaching material and videos

Live Cell Imaging Core Facility instruction videos and tutorials.

Typical workflows

Typical workflow of how to set a camera-based system (PDF, 578.81 KB)

Typical workflow how to set the parameters for a single point confocal (PDF, 636.47 KB)

Excitation and emission forms

Evaluation of bleedthrough form: Dragon, Pegasus - Single point scanning confocals (File, 33.22 KB)

Evaluation of bleedthrough form: Victor - Widefield excitation and emission 20241030 (File, 33.46 KB)

Evaluation of bleedthrough form: Victor multipoint confocal - excitation and emission (File, 32.83 KB)

Evaluation of bleedthrough form: Orion - Widefield and spinning disk and DeepSIM (File, 31.03 KB)

Evaluation of bleedthrough form: Tweety - Widefield and Multipoint confocal (File, 33.48 KB)

Evaluation of bleedthrough form: Aurora - Light sheet (File, 31.65 KB)

Evaluation of bleedthrough: template (File, 34.2 KB)

Teaching material

How to avoid bleedthrough

Bit depth, saturation and underexposure

Introduction to image analysis using NIS-Elements

Sample preparation: introduction

Basic in Nikon NIS-Elements for confocal microscopy

Setting up Koehler illumination

How to find your sample without bleaching it

Practical tips for sample preparation and imaging