3D-EM

Cellular electron microscopy has been a key technique for understanding the cell’s core elements and mechanisms since the 1950s. Electron microscopic methods have laid the foundation for a number of Nobel Prizes and have given us much of the basic understanding of human cells’ overall structure, physiology and mechanisms—basically the foundation of all modern medicine.

In recent years, electron microscopy has undergone extremely rapid development through a combination of significantly better optics, sample processing, detectors and image processing. The development has given rise to the so-called “Resolution Revolution,” which in recent years has gradually increased the average resolution of biological preparations significantly. Part of the discoveries and methodology resulted in a Nobel Prize to Richard Henderson, Jaques Dubochet and Joachim Frank. At the beginning of this revolution, a visionary decision of the then Karolinska Institutet’s (KI’s) leadership was made to build premises for a future electron microscopy center nearby the Biomedicum building at the KI Solna campus. These premises have now been built specifically for high-end biomedical electron microscopy. During 2019-2020 we will equip the newly built KI 3D-EM center with the best possible equipment and thereby enable a much broader group of KI researchers to utilize the enormous advances in electron microscopy in recent years.


Image: The new KI 3D-EM facility is located in the Wargentin building.


Images: The 300 kV FEG microscope room and the 200 kV FEG microscope room.

 

Instruments available

Electron microscopes

To be purchased:

  • One 300 kV FEG
  • One 120 kV LaB6
  • One cryo-FIB-SEM, optionally one 200 kV FEG.

Sample preparation

Available:

  • Leica Microtome 1
  • Leica Microtome 2
  • Vitrobot Mk 1
  • Glow discharger
  • Plasma cleaner

Access and booking

Please contact facility manager Sergej Masich. We will utilise the central KI core-facility booking system once that is available.

Teaching and training

We will arrange courses and workshops. In the beginning, teaching and hands-on-training on specimen preparation and microscope operation will be provided to smaller groups or on a on-to-one basis.

Prices for service

To be decided but will reflect the relative costs for maintaining the respective instruments. Furthermore, pricing for industry access will attain full-cost coverage.

Advisory board

Name Title Department
Stefan Eriksson (chair)  Lecturer senior Department of Physiology and Pharmacology
Martin Hällberg Senior researcher Department of Cell and Molecular Biology
Björn Högberg Docent Department of Medical Biochemistry and Biophysics
Luca Jovine Professor Department of Biosciences and Nutrition
Lennart Nilsson Professor Department of Biosciences and Nutrition
Gunter Schneider Professor Department of Medical Biochemistry and Biophysics
Oleg Shupliakov Professor Department of Neuroscience

Contact us


Facility director
Martin Hällberg
E-mail: Martin.Hallberg@ki.se

Facility manager
Sergej Masich
E-mail: Sergej.Masich@ki.se
Core facilitet