Cellular electron microscopy has been a key technique for understanding the cell’s core elements and mechanisms since the 1950s. Electron microscopic methods have laid the foundation for a number of Nobel Prizes and have given us much of the basic understanding of human cells’ overall structure, physiology and mechanisms—basically the foundation of all modern medicine.
In recent years, electron microscopy has undergone extremely rapid development through a combination of significantly better optics, sample processing, detectors and image processing. The development has given rise to the so-called “Resolution Revolution,” which in recent years has gradually increased the average resolution of biological preparations significantly. Part of the discoveries and methodology resulted in a Nobel Prize to Richard Henderson, Jaques Dubochet and Joachim Frank. At the beginning of this revolution, a visionary decision of the then Karolinska Institutet’s (KI’s) leadership was made to build premises for a future electron microscopy center nearby the Biomedicum building at the KI Solna campus. These premises have now been built specifically for high-end biomedical electron microscopy. During 2019-2020 we will equip the newly built KI 3D-EM center with the best possible equipment and thereby enable a much broader group of KI researchers to utilize the enormous advances in electron microscopy in recent years.
Available for use:
120 kV LaB6 microscope: Talos 120C G2 with Ceta-D detector
300 kV FEG microscope: Krios G3i with Gatan K3 detector and Ceta-D detector.
Aquilos 2 cryo-FIB-SEM
Leica SP8 cryo-confocal microscope
- Leica Microtome 1
- Leica Microtome 2
- Vitrobot Mk 1
- Vitrobot Mk 4
- Glow discharger
- Plasma cleaner
Access and booking
Only registered users can have an access to the facility. Registration is to be done at 3D-EM Core Facility Service and must be approved by PI of the user. The PI provides each user with project number to facilitate the payment via iLab booking system.
Teaching and training
We will arrange courses and workshops. However, users are encouraged to receive personal training followed by a test that will open the access to the 120 kV Talos microscope. Please contact Sergej.Masich@ki.se for more details.
Prices for service
Prices for service and access for equipment currently available:
- Training is free for KI personnel. However, the microscope time is charged at normal rate.
- Talos 120C G2 cost 200 SEK per hour with minimal booking time of 3 hrs.
- Krios G3i with K3 cost 5000 SEK per 24h slot or 2000 SEK per 3h Autogrid screening slot.
- Consultant work, assistance outside training or training non-KI personnel 550 SEK per hour.
- Usage of sample preparation equipment 100 SEK per hour with minimal booking time 2 hrs.
Pricing for other academic access/training is twice the prices stated above (except hourly staff time charge). Since the internal prices are subsidized by KI they are reserved for KI groups only with personnel that are employed by KI and where all accumulated costs are internally billed. Furthermore, only KI personnel may be the main operators of the Krios so staff setup time will typically need to be added to the total cost of access for external academic users. KI personnel are defined as employed by KI or associated hospitals and where all accumulated costs are internally billed i.e. not to clinics or other universities. All prices are subject to local KI overhead (currently 22.5%). For industry access, please contact us.
Publications resulting from 3D-EM facility use
Structural basis for late maturation steps of the human mitoribosomal large subunit
Cipullo M, Gese GV, Khawaja A, Hällberg BM*, Rorbach J*.
Nature Communications, June 16, 2021, doi: 10.1038/s41467-021-23617-8.
Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape.
Koenig PA, Das H, Liu H, Kümmerer BM, Gohr FN, Jenster LM, Schiffelers LDJ, Tesfamariam YM, Uchima M, Wuerth JD, Gatterdam K, Ruetalo N, Christensen MH, Fandrey CI, Normann S, Tödtmann JMP, Pritzl S, Hanke L, Boos J, Yuan M, Zhu X, Schmid-Burgk JL, Kato H, Schindler M, Wilson IA, Geyer M, Ludwig KU, Hällberg BM, Wu NC, Schmidt FI
Science 2021 02;371(6530):
Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch.
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep 2020 Dec;33(12):108529
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2.
Custódio TF, Das H, Sheward DJ, Hanke L, Pazicky S, Pieprzyk J, et al
Nat Commun 2020 11;11(1):5588
A latent lineage potential in resident neural stem cells enables spinal cord repair.
Llorens-Bobadilla E, Chell JM, Le Merre P, Wu Y, Zamboni M, Bergenstråhle J, et al
Science 2020 10;370(6512):
An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction.
Hanke L, Vidakovics Perez L, Sheward DJ, Das H, Schulte T, Moliner-Morro A, et al
Nat Commun 2020 09;11(1):4420