FENO - our offer
The Morphological Phenotype Analysis Core Facility (FENO) at Campus Flemingsberg, is a well-established core facility in service since 2002. FENO provides professional research support in animal experiments, including histopathology, a wide range of stainings, IHC, RNAScope and digitalization for different species.
Our service
- Histopathology services for pathological feature characterization in tissues and organs for different species.
- Tissue processing and sectioning using microtome/cryotome, staining.
- Immunohistochemistry on frozen/paraffin embedded tissues.
- Immunohistochemistry on frozen/paraffin embedded tissues using automated Leica BOND RXm.
- RNAScope (InSitu hybridization) on frozen/paraffin embedded tissues.
- Bright field scanning - slide digitalization.
Our method and techniques
Method | Instrument/technique |
---|---|
Study design, planning, autopsy | FENO consultation |
Registration | iLab |
Tissue dehydration /paraffinization | Automatic/manual |
Sectioning: paraffin | 4 x Thermo microtome |
Sectioning: cryo | Leica cryotome |
Histochemical routine staining (HE) | Manual |
Histochemical special staining | Tiziano robot/manual |
Immunohistochemical staining (chromogenic /fluorescent/multiplex) | Leica Bond RXm robot/ manual |
RNA in situ hybridization | Leica bond RXm/manual-RNA scope |
Histologic image documentation | Pannoramic 3D histech section scanner 20x ZEISS Axioscan 7 scanner (bright field, polarization and fluorescence function) |
Online sharing of images/scanned sections | FENO server |
Histological stains
- H&E
- Special Stains
- Special stains and immunostaining multiplexing
Immunostaining
- Automated Immunohistochemistry (IHC) – Single and Dual
- Automated Immunofluorescence (IF)
- Automated in situ hybridization (ISH/FISH)
Four examples of staining: H&E, Sirius Red, PAS and ORO.