FENO - our offer
The Histological Core Facility – FENO at Campus Flemingsberg, is a well-established core facility in service since 2002. FENO provides professional research support in animal experiments, including histopathology, a wide range of stainings, IHC, IF, Opal and RNAScope and digitalization.
Our service
- Histopathology services in tissues and organs for different species.
- Tissue processing and sectioning using microtome/cryotome.
- Routine and special stainings.
- Immunohistochemistry on frozen/paraffin embedded tissues.
- Immunohistochemistry on frozen/paraffin embedded tissues using automated Leica BOND RXm.
- RNAScope (InSitu hybridization) on frozen/paraffin embedded tissues.
- Bright field scanning - slide digitalization.
Our methods and techniques
| Method | Instrument/technique |
|---|---|
| Study design, planning | FENO consultation |
| Registration | iLab |
| Tissue dehydration /paraffinization | Automatic |
| Sectioning: paraffin | 4 x Thermo microtome |
| Sectioning: cryo | Leica cryotome |
| Histochemical routine staining (HE) | Manual |
| Histochemical special staining | Manual |
| Immunohistochemical staining (chromogenic /fluorescent/multiplex) | Leica Bond RXm robot/ manual |
| RNA Scope in situ hybridization | Leica bond RXm/manual-RNA scope |
| Histologic image scanning | ZEISS Axioscan 7 scanner (bright field, polarization and fluorescence function) Pannoramic D histech section scanner 20x |
| Online sharing of images/scanned sections | OneDrive server |
| Study documentation | KI ELN - The electronic notebook |
Histological stains
- H&E
- Special Stainings: PAS, Sirius Red, Oil Red O
Immunostaining
- Automated Immunohistochemistry (IHC) – Single and Dual
- Automated Immunofluorescence (IF)
- Opal 4 and 7-Color
- Automated in situ hybridization (ISH/FISH)
Four examples of staining: H&E, Sirius Red, PAS and ORO.
Price list for KI researchers
| Service | Price |
|---|---|
| Consultation (first consultation is free) | 1,000 SEK/hour |
| Tissue preparation | 1,000 SEK/hour |
| Embedding in paraffin | 1,000 SEK/hour |
| Dehydration | 50 SEK/cassette |
| Paraffin sectioning | 50 SEK/slide |
| Cryo sectioning | 65 SEK/slide |
| Special parfaffin/cryo sectioning or ≤30 sections | 1,000 SEK/hour |
| H&E staining | 75 SEK/slide |
| Special stains: PAS, Sirius Red, Perl's | 100 SEK/slide |
| IHC/IF staining – Manually | 900 SEK/hour |
| IHC, IF, Opal, RNAScope staining Self-service Leica BOND - up to 10 slides (excl. Antibodies, kits, reagents) | 2,000 SEK/run |
| IHC, IF, Opal, RNAScope staining FENO performs staining on Leica BOND RXm | 500 SEK/slide |
| Scanning (*extra charge if new profiles needs to be created) | 1,000 SEK/hour |
| Slide Box | 100–200 SEK |
| Urgent work – extra charge | 1,000 SEK |