BFC - Flow Cytometry Sorting
Flow Cytometry Sorting involves using a flow cytometer with cell sorting capability to interrogate cells stained with fluorescent markers, classifying them into groups, and then physically separate the groups into test tubes or multi-well plates.
Cell sorting at the BFC can be done as an assisted sort (run by staff) or as a self-user sort.
Assisted sort
Staff members of the facility can sort samples for users requiring it. Please, refer to iLab and create a Work Request under the Request Services tab. We will contact you to arrange a pre-sort meeting to discuss matters of the sort. After this, a timeslot can be booked in the instrument’s calendar.
Self-user sort
If your project requires frequent and recurrent usage of the sorters, a training course can be requested. There are two options to obtain a driver’s license for our cell sorters:
- Demonstrating previous experience and competence on Aria or Cytoflex SRT sorter by passing the Driver’s license test
- By getting trained in the facility
Request training
To request a spot in our Sorting training courses:
- Create a Training Request on iLab (Request Services > Introduction and training request: Sorters).
- The initial training consists of five hours theory and six hours hands-on training, covering different aspects of using the instrument.
- This is followed by three (or more) sessions of individual practice, to gain competence to take the Driver´s License Test
- After passing the Driver's License Test, the user will get access to self-operated and out-of-hours sorts.