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Morphological Phenotype Analysis (FENO)

The great surge in numbers of mutant mice has made efficient phenotyping a necessity in order to find and adequately describe the changes caused by the genetic manipulation. Pathology is a major and integral part of the phenotyping process.

With support from WCN, The Unit for Morphological Phenotype Analysis (FENO) was established as a competence and service facility for mouse pathology at KI in 2002, and as such specializing in the pathology of genetically engineered mice.

FENO offers

  • A full necropsy and histopathology service to analyse and describe pathological changes in tissues and organs - with attention for species differences.
  • Processing of tissues, stainings, and qualified pathologic analysis, separate or in combination.
  • Immunohistochemistry on frozen/paraffin embedded tissues.
  • Photograhic registration of results including fluorescent microscopic methods.
  • Slide digitalisation and image analysis.
  • Microscopic analysis of prenatal lethal mutants.
  • Advice on protocols: animal selection and tissue sampling, breeding schemes.

We have a fixed pricelist.

Contact

Raoul Kuiper

Board certified in animal pathology
H5 Department of Laboratory Medicine

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2 images of mouse liver
2 images of mouse liver, one stained using markers for macrophages (green) and Tcells (red); DAPI in blue. Staining was performed on a Leica Bond. The inset is a HE brightfield image of a similar ndular lesion in another section. The image shows a pathobiological correlation of a phenomenon that looks more cryptic in fluorescence. Photo: Raoul Kuiper
Scientific image: RNA-scope™ for EgFP on Leica Bond RXm using Leica's red staining kit.
RNA-scope™ for EgFP on Leica Bond RXm using Leica's red staining kit. Image of mouse subcutis with neoplastic growth of genically altered and EGFP marked mamary epithelial cells Images shows fluorescent and brightfield microscopy of the same slide. Photo: Raoul Kuiper
Scientific image: The image is a combined image of normal bright field microscopy (upper left) with spectral brieght field microscopy using the Vectra 3 system (Akoya Biosciences)
Mouse mamary tubule marked with EGFP, chromogenic RNAscope™ on Leica Bond RXm. The image is a combined image of normal bright field microscopy (upper left) with spectral bright field microscopy using the Vectra 3 system (Akoya). The image shows the higher sensitivity achieved by spectral imaging of the same slide. Photo: Raoul Kuiper