MedH Flow Cytometry Core Facility

The MedH Flow Cytometry Core Facility at Karolinska Institutet (KI) provides KI and Karolinska University Hospital researchers opportunity for assisted and non-assisted flow cytometry analysis and cell sorting.

Picture from MedH Facs
Flow Cytometry Facility at Karolinska Institutet

Instruments

The facility is equipped with the following instruments for both assisted and unassisted analysis and cell sorting experiments.

Instrument: FACS Aria and Facs Fusion. See configuration below
Sorter Specification Price for KI users Price for other academic institutions Price for commercial users
SORP BD FACSAria (Sansa) FACSA 18 parameters, 5 Lasers: 355*, 405, 488, 561 and 633* nm * co-linear 390 SEK/hr for assisted and non-assisted 980 SEK/hr 1960 SEK/hr
SORP BD FACSAria Fusion (Arya) 20 parameters, 5 Lasers: 355, 405, 488, 561 and 640 nm 390 SEK/hr for assisted and non-assisted 980 SEK/hr 1960 SEK/hr

Last revision: Jan 1, 2019 Minimum time for sorting is 1 hr. 

 

Analysis Specification Price for KI users Price for other academic institutions Price for commercial users
SORP BD LSRFortessa 20 parameters, 4 Lasers: 355, 405, 488, 561 and 640 nm 300 SEK/hr 480 SEK/hr 960 SEK/hr
SORP BD Symphony A5 30 parameters, 5 Lasers: 355, 405, 488,561 and 637 nm 335 SEK/hr 520 SEK/hr 1040 SEK/hr
CytoFLEX S (Beckman Coulter) 13 parameters, 4 Lasers: 405, 488, 561 and 638 nm 280 SEK/hr 450 SEK/hr 900 SEK/hr

Last revision: Jan 1, 2019 Minimum time for analysing is 30 min. Integrated 96 well plate module for High-Throughput analysis

Access to the MedH FACS Facility

Please write a short email about your needs to grp_herm_facsfacility@ki.se . We will then contact you and arrange a meeting to discuss your needs and have we can help you.

Online booking system

This system will be used for all bookings and service requests MedH Flow Cytometry Core Facility

For instructions on how to create an account and to make reservations:

Weekly user meeting

The FACS facility arrange a digital user meeting. The purpose is to initiate a dialogue between user and Facility. This is the forum for the user to ask question, give feedback to the Facility, and present and discus FACS result. The Facility staff will inform about the status on the instrument, upcoming event, and new SOP.

Acknowledgement

In order to document usage of the MedH FCF, which is important when securing core funding, the users are encouraged to acknowledge the MedH FCF in publications in which data generated in the facility are included. As a core facility, there are no expectations with regard to co-authorships for the staff operating the facility (unless the users think this is scientifically warranted).

Please use the following statement for acknowledging the MedH FACS facility:

“We would like to acknowledge the MedH Flow Cytometry core facility (Karolinska Institutet), supported by KI/SLL, for providing cell sorting services**/cell analysis services**/technical expertise**/scientific input**”.

**delete as required.

Visiting address

The MedH Flow Cytometry Core Facility is situated at the 7th flour, elevator N of the NEO research park in Huddinge, adjacent to Karolinska University Hospital in Huddinge.

Map MedH Flow Cytometry Core Facility

Contact

Postal address:

MedH Flow Cytometry Facility
NEO HERM Lab 7130, Blickagången 16, SE-141 83 Stockholm

Email:

grp_herm_facsfacility@ki.se

Personnel

Belinda Pannagel

Research technician

Mahin Nikougoftar Zarif

Research technician

Scientfic director

Petter Woll

Scientific director, Ph.D., Assistant Professor