MedH Flow Cytometry Core Facility
The MedH Flow Cytometry Core Facility at Karolinska Institutet (KI) provides an opportunity for assisted and non-assisted flow cytometry analysis and cell sorting for KI, Karolinska University Hospital researchers as well as users from other universities and companies.
Services and equipment
BD Discover S8
Discover S8 which is equipped with 5 lasers can detect 78 parameters and has 5 imaging detectors. This enables for high throughput single cell imaging along with fast single cell sorting both in plate and tube mode.
BD FACS Aria Fusion
BSL2 BD FACS Aria fusion is equipped with 5 lasers (355, 405, 488, 561 and 640 nm) detecting 20 parameters. Cells can be sorted out both in tube and plate mode like 96-well, 384-well plates along with 60 and 72-well terasaki plates, eppendorf, FACS, PCR and 15ml falcon tubes.
BD Symphony A5
This high parameter cell analyzer is equipped with 5 lasers (355, 405, 488, 561 and 637 nm) which can detect 30 parameters. Cells can be analyzed both in tube and plate mode.
BD LSRFortessa
LSRFortessa from BD is equipped with 5 lasers (355, 405, 488, 561 and 637 nm) and can detect 20 parameters. Cells can be analyzed both in tube and plate mode.
CytoFLEX S
This user-friendly BC Cytoflex is equipped with 4 lasers (405, 488, 561 and 638 nm) and can detect 13 parameters. Here also, cells can be analyzed both in tube and plate mode.
Upcoming events
Publications
A list of publications by the researchers using the MedH Flow Cytometry Core Facility.
Facts about MedH Flow Cytometry Core Facility
Location: NEO, Medicinaren 25, HERM floor 7, Blickagången 16, Huddinge
Scientific director: Petter Woll
Managers: Belinda Pannagel, Narmadha Subramanian
Key words: Flow cytometry, cell sorter, flow cytometer analyzers, lasers, image-based sorter, spectral, plate and tube mode, data-analysis
Department: Department of Medicine, Huddinge
