MedH Flow Cytometry Core Facility
The MedH Flow Cytometry Core Facility at Karolinska Institutet (KI) provides an opportunity for assisted and non-assisted flow cytometry analysis and cell sorting for KI, Karolinska University Hospital researchers as well as users from other universities and companies.
![Picture from MedH Facs](/sites/kise/files/styles/fullsize_colour_no_crop/public/qbank/Facs_bild_custom20240119102557.webp?itok=r7SoFhKQ)
Services and equipment
![Spectral image-based sorter Discover S8 in MedH Flow Cytometry Core Facility.](/sites/kise/files/styles/article_full_width/public/qbank/BDFACSDiscoverS8_MedHFCCF_20240129_custom20240130102807.jpg?itok=y5pyKLmj)
BD Discover S8
Discover S8 which is equipped with 5 lasers can detect 78 parameters and has 5 imaging detectors. This enables for high throughput single cell imaging along with fast single cell sorting both in plate and tube mode.
![Sorter BD FACS Aria Fusion in MedH Flow Cytometry Core Facility.](/sites/kise/files/styles/article_full_width/public/qbank/BDFACSAriaFusion_MedHFCCF_20240129_custom20240130102717.jpg?itok=yxtYh5pQ)
BD FACS Aria Fusion
BSL2 BD FACS Aria fusion is equipped with 5 lasers (355, 405, 488, 561 and 640 nm) detecting 20 parameters. Cells can be sorted out both in tube and plate mode like 96-well, 384-well plates along with 60 and 72-well terasaki plates, eppendorf, FACS, PCR and 15ml falcon tubes.
![Analyzer Symphony A5 in MedH Flow Cytometry Core Facility.](/sites/kise/files/styles/article_full_width/public/qbank/BDSymphonyA5_MedHFCCF_20240129_custom20240130103022.webp?itok=LUmwsMno)
BD Symphony A5
This high parameter cell analyzer is equipped with 5 lasers (355, 405, 488, 561 and 637 nm) which can detect 30 parameters. Cells can be analyzed both in tube and plate mode.
![Analyzer LSRFortessa in MedH Flow Cytometry Core Facility.](/sites/kise/files/styles/article_full_width/public/qbank/LSRFortessa_MedHFCCF_20240129_custom20240130102911.jpg?itok=QAtWjWnf)
BD LSRFortessa
LSRFortessa from BD is equipped with 5 lasers (355, 405, 488, 561 and 637 nm) and can detect 20 parameters. Cells can be analyzed both in tube and plate mode.
![Analyzer Cytoflex in MedH Flow Cytometry Core Facility.](/sites/kise/files/styles/article_full_width/public/qbank/Cytoflex_MedHFCCF_20240129_custom20240130103213.webp?itok=y7RBBFCd)
CytoFLEX S
This user-friendly BC Cytoflex is equipped with 4 lasers (405, 488, 561 and 638 nm) and can detect 13 parameters. Here also, cells can be analyzed both in tube and plate mode.
Upcoming events
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Publications
A list of publications by the researchers using the MedH Flow Cytometry Core Facility.
![Colourful steps](/sites/kise/files/styles/article_full_width/public/qbank/Neo_stairs8_custom20240119103105.webp?itok=8LhretQe)
Facts about MedH Flow Cytometry Core Facility
Location: NEO, Medicinaren 25, HERM floor 7, Blickagången 16, Huddinge
Scientific director: Petter Woll
Managers: Belinda Pannagel, Narmadha Subramanian
Key words: Flow cytometry, cell sorter, flow cytometer analyzers, lasers, image-based sorter, spectral, plate and tube mode, data-analysis
Department: Department of Medicine, Huddinge
![](/sites/kise/files/styles/article_full_width/public/qbank/kiflagga3346_custom20240115134001.jpg?itok=sCNb2xJq)