MedH Flow Cytometry Core Facility
The MedH Flow Cytometry Core Facility at Karolinska Institutet (KI) provides KI and Karolinska University Hospital researchers opportunity for assisted and non-assisted flow cytometry analysis and cell sorting.
Instruments
The facility is equipped with the following instruments for both assisted and unassisted analysis and cell sorting experiments. Minimum time for sorting or analysing is 1 hr.
| Sorter | Specification | Price for KI users | Price for other academic institutions | Price for commercial users |
|---|---|---|---|---|
| SORP BD FACSAria Fusion (Sansa) | 18 parameters, 5 Lasers: 355*, 405, 488, 561 and 633* nm * co-linear | 390 SEK/hr for assisted and non-assisted | 980 SEK/hr | 1960 SEK/hr |
| SORP BD FACSAria Fusion (Arya) | 20 parameters, 5 Lasers: 355, 405, 488, 561 and 640 nm | 390 SEK/hr for assisted and non-assisted | 980 SEK/hr | 1960 SEK/hr |
Last revision: Jan 1, 2019.
Configurations FACSAria Fusion Sansa and Arya
| Analysis | Specification | Price for KI users | Price for other academic institutions | Price for commercial users |
|---|---|---|---|---|
| SORP BD LSRFortessa | 20 parameters, 4 Lasers: 355, 405, 488, 561 and 640 nm | 300 SEK/hr | 480 SEK/hr | 960 SEK/hr |
| SORP BD Symphony A5 | 30 parameters, 5 Lasers: 355, 405, 488,561 and 637 nm | 335 SEK/hr | 520 SEK/hr | 1040 SEK/hr |
| CytoFLEX S (Beckman Coulter) | 13 parameters, 4 Lasers: 405, 488, 561 and 638 nm | 280 SEK/hr | 450 SEK/hr | 900 SEK/hr |
Last revision: Jan 1, 2019.
Configurations LSR Fortessa, Bd Symphony and Cytoflex
Access to the MedH FACS Facility
The first step to get access to the MedH FACS Facility is to create an account on iLab (more information below). After creating an account, please write a short email about your needs to facs@medh.ki.se. We will contact you and arrange for a meeting to discuss your needs, how we can help you and which are next steps to become an independent user.
Account and online booking system
This system will be used for all bookings and service requests MedH Flow Cytometry Core Facility.
For instructions on how to create an account and to make reservations:
- Instructions for KI users
- Instructions for corporate users
- Instructions for PI's/group leaders
- GDPR_Personuppgiftsavtal: Flow Cytometry
If you encounter any problems, please contact Ilab support email: UF CFM-support cfm-support@ki.se
Acknowledgement
In order to document usage of the MedH FCF, which is important when securing core funding, the users are encouraged to acknowledge the MedH FCF in publications in which data generated in the facility are included. As a core facility, there are no expectations with regard to co-authorships for the staff operating the facility (unless the users think this is scientifically warranted).
Please use the following statement for acknowledging the MedH FACS facility:
“We would like to acknowledge the MedH Flow Cytometry core facility (Karolinska Institutet), supported by KI/SLL, for providing cell sorting services**/cell analysis services**/technical expertise**/scientific input**”.
**delete as required.
Contact us
Visiting address:
Medicinaren 25/Neo
HERM floor 7
Blickagången 16
141 52 Huddinge
Email:
facs@medh.ki.se
Phone:
+46 70-091 48 10
Belinda Pannagel
Facility manager