MedH Flow Cytometry Core Facility - our offer

The MedH Flow Cytometry Core Facility at Karolinska Institutet (KI) provides an opportunity for assisted and non-assisted flow cytometry analysis and cell sorting for KI, Karolinska University Hospital researchers as well as users from other universities and companies.

The MedH Flow Cytometry Core Facility is equipped with the following instruments for non-assisted analysis and both assisted and non-assisted cell sorting experiments. Minimum time for sorting or analyzing is one (1) hour.

Sorters

Spectral image-based sorter Discover S8 in MedH Flow Cytometry Core Facility.
BD Discover S8 (Spectral and Imaging).

BD Discover S8 (Spectral and Imaging)

This spectral image-based sorter with BD cell view image technology is a full spectral analyzer where cells can be sorted based on images and using imaging parameters like eccentricity, max intensity, size, radial moment, correlation, and delta center of mass. Discover S8 which is equipped with 5 lasers can detect 78 parameters and has 5 imaging detectors. This enables for high throughput single cell imaging along with fast single cell sorting both in plate and tube mode. Sorter is located within a safety cabinet enabling the usage of BSL1 and BSL2 samples.

Specification:

78 parameters, 5 Lasers: 355, 405, 488, 561 and 633

Fees (as from 1 August 2025):

  • KI users: 550 SEK/hr for assisted and non-assisted
  • Other academic institutions: 1,100 SEK/hr
  • Commercial users: 2,280 SEK/hr
Sorter BD FACS Aria Fusion in MedH Flow Cytometry Core Facility.
BD FACS Aria Fusion.

BD FACS Aria Fusion

BSL2 BD FACS Aria fusion is equipped with 5 lasers (355, 405, 488, 561 and 640 nm) detecting 20 parameters. Cells can be sorted out both in tube and plate mode like 96-well, 384-well plates along with 60 and 72-well terasaki plates, eppendorf, FACS, PCR and 15ml falcon tubes. Aria fusion is located within a safety cabinet enabling the usage of BSL1 and BSL2 samples.

Specification:

19 parameters, 5 Lasers: 355, 405, 488, 561 and 633

Fees (as from 1 August 2025)

  • KI users: 430 SEK/hr for assisted and non-assisted
  • Other academic institutions: 1,080 SEK/hr
  • Commercial users: 2,160 SEK/hr

Configurations FACS Aria Fusion and Discover S8

Analyzers

Analyzer Symphony A5 in MedH Flow Cytometry Core Facility.
Cell analyzer BD Symphony A5.

BD Symphony A5

This high parameter cell analyzer is equipped with 5 lasers (355, 405, 488, 561 and 637 nm) which can detect 30 parameters. Cells can be analyzed both in tube and plate mode.

Specification:

30 parameters, 5 Lasers: 355, 405, 488,561 and 637 nm

Fees (as from 1 August 2025):

  • KI users: 370 SEK/hr
  • Other academic institutions: 570 SEK/hr
  • Commercial users: 1,140 SEK/hr
Analyzer LSRFortessa in MedH Flow Cytometry Core Facility.
BD LSRFortessa.

BD LSRFortessa

LSRFortessa from BD is equipped with 5 lasers (355, 405, 488, 561 and 637 nm) and can detect 20 parameters. Cells can be analyzed both in tube and plate mode.

Specification:

20 parameters, 4 Lasers: 355, 405, 488, 561 and 640 nm

Fees (as from 1 August 2025):

  • KI users: 330 SEK/hr for assisted and non-assisted
  • Other academic institutions: 530 SEK/hr
  • Commercial users: 1,060 SEK/hr
Analyzer Cytoflex in MedH Flow Cytometry Core Facility.
CytoFLEX S (Beckman Coulter).

CytoFLEX S (Beckman Coulter)

This user-friendly BC CytoFLEX S is equipped with 4 lasers (405, 488, 561 and 638 nm) and can detect 13 parameters. Here also, cells can be analyzed both in tube and plate mode.

Specification:

13 parameters, 4 Lasers: 405, 488, 561 and 638 nm

Fees (as from 1 August 2025):

  • KI users: 310 SEK/hr
  • Other academic institutions: 500 SEK/hr
  • Commercial users: 990 SEK/hr

Configurations