KIGene - Genetic Analysis and Spatial Biology
KIGene Core Facility offers genetic analyses, covering Sanger DNA sequencing, miRNA profiling, gene expression and genotyping, as well as spatial profiling of RNA and proteins on tissue sections.
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Photo: Oxford Nanopore TechnologiesLong read sequencing!
We have recently established the PromethION 2 Integrated platform from Oxford Nanopore Technologies. Nanopore sequencing enables real-time analysis of DNA and RNA by measuring changes in electrical current as molecules pass through nanopores. The method supports ultra-long reads and can simultaneously detect epigenetic modifications.
We offer a full-service option, as well as the opportunity to operate the platform independently after completing an introductory training course provided by core staff. The latter option is more cost-effective and also allows users to adjust parameters during the sequencing run.
For more information, please contact us at kigene@mmk.ki.se.
Photo: N/ASpatial Biology
We offer full service assignments of high-plex spatial profiling of tissue sections via the nanoString/Bruker GeoMx and CosMx platforms. Our cryostat and light microscope are offered on a “hands-on” basis.
Photo: Gerd Altmann PixabayGenetic Analysis
KIGene is a well-established node at Karolinska Institutet with more than thirty years experiences of running a core facility. We offer genetic analyses, covering DNA sequencing, gene expression, genotyping, miRNA profiling and copy number variation, as well as epigenetic analysis.
Photo: Ida NilssonFacts about KIGene Core Facility
Location: Karolinska University Hospital, Solna
Scientific Director: Martin Schalling
Keywords:
Department: Department of Molecular Medicine and Surgery
Photo: Lasse Skog