Biomedicum Flow Cytometry Core Facility

The Biomedicum flow cytometry core facility (BFC) is a shared resource laboratory that will provide researchers with the capability of performing a wide range of flow cytometry experiments.

Biomedicum Facs facility
Biomedicum Facs facility

Location: Biomedicum, Quarter 3C (entrance level)

Instruments

The facility is equipped with the following instruments for both assisted and unassisted analysis and cell sorting experiments. Instrument configurations (excel file)

Analysis

Instrument Laser specification Feasible parameters KI users Other academic institutions PRICE (SEK) Commercial users
BD LSRII SORP 4 lasers: 405, 488, 532, 640nm 18 colors + FSC, SSC Unassisted Assisted 530 880 670
1120
1060
1760
BD FACS Verse 3 lasers: 405, 488, 640 nm 8 colors + FSC, SSC Unassisted Assisted 440 790 550
1000
880
1580
FACS Calibur 2 lasers: 488 & 633nm 4 colors + FSC, SSC Unassisted Assisted 350 700 450
900
700
1400
CyAn ADP 3 lasers: 405, 488, 635nm 9 colors + FSC, SSC Unassisted Assisted 440 790 550
1000
880
1580
FACSCanto II 3 lasers: 405, 488, 633nm 8 colors + FSC, SSC Unassisted Assisted 440 790 550
1000
880
1580

Cell Sorting

Instrument Laser specification Feasible parameters KI users Other academic institutions PRICE (SEK) Commercial users
BD FACSAria Fusion 4 lasers: 405, 488, 561, 640nm 16 colors + FSC, SSC Unassisted Assisted 630 980 630
980
1260
1960
BD FACSJazz 3 lasers: 488, 561, 640nm 6 colors + FSC, SSC Unassisted Assisted 630 980 800
1250
1260
1960
FACSAria III 4 lasers: 405, 488, 561, 633nm 11 colors + FSC, SSC Unassisted Assisted 630 980 800
1250
1260
1960

How to get access to the BFC

To get access to the BFC, the following steps have to be taken:

A) Apply for an account in the iLab booking system. This system is used for all bookings and requests. Instructions on how to apply for a iLab account can be found below.

B) Attend a “General introduction to the BFC” session (30 min)
This session will give information on how the facility is organized and to the rules and routines of the facility. After attending this introduction session you will get access to the facility with your key-card. You will also get added to the BFC-mailing list to receive information about the facility. The introduction sessions are arranged every 2-3 weeks.

C) Get driver’s license to run instruments
For analysis instruments a driver’s license can be obtained either by demonstrating previous experience on a particular instrument or by getting trained in the facility. For BD Aria sorters we periodically organise operator training courses, which consist of a theoretical and hands on 2-day course. Once this training is completed all users should practise and discuss their needs within the following 2-3 weeks so they get used to general routines and show competence to sort autonomously. As soon as the drive’s license is obtained, access to iLabs booking system will be permitted. Contact us for pricing and to schedule any training course or get a driver’s license.

Online booking system

From August 18, 2020, the facility will switch to the iLab online booking system.

This system will be used for all bookings, service requests as well as introduction/training. Core iLab page

For instructions on how to create an account and to make reservations:

More information about implementation of iLab at KI.

Personnel

Saikiran Sedimbi

Facility director
Facs Facilitet
C1 Department of Microbiology, Tumor and Cell Biology

Maria Johansson

Facility manager
Kärre
C1 Department of Microbiology, Tumor and Cell Biology

Juan Basile

Research engineer
Facs Facilitet
C1 Department of Microbiology, Tumor and Cell Biology