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Biomedicum Flow Cytometry Core Facility

Biomedicum Facs facility

The Biomedicum flow cytometry core facility (BFC) is a shared resource laboratory that will provide researchers with the capability of performing a wide range of flow cytometry experiments.

Location: Biomedicum, Quarter 3C (entrance level)

Instruments

The facility is equipped with the following instruments for both assisted and unassisted analysis and cell sorting experiments.

Instrument configurations (excel file)

Analysis

PRICE (SEK)
Instrument Laser specification Feasible parameters KI users Other academic institutions Commercial users
BD LSRII SORP 4 lasers: 405, 488, 532, 640nm 18 colors + FSC, SSC

Unassisted

Assisted

530

880

670

1120

1060

1760

BD FACS Verse 3 lasers: 405, 488, 640 nm 8 colors + FSC, SSC

Unassisted

Assisted

440

790

550

1000

880

1580

FACS Calibur 2 lasers: 488 & 633nm 4 colors + FSC, SSC

Unassisted

Assisted

350

700

450

900

700

1400

CyAn ADP 3 lasers: 405, 488, 635nm 9 colors + FSC, SSC

Unassisted

Assisted

440

790

550

1000

880

1580

FACSCanto II 3 lasers: 405, 488, 633nm 8 colors + FSC, SSC

Unassisted

Assisted

440

790

550

1000

880

1580

Cell Sorting

PRICE (SEK)
Instrument Laser specification Feasible parameters KI users Other academic institutions Commercial users
BD FACSAria Fusion 4 lasers: 405, 488, 561, 640nm 16 colors + FSC, SSC

Unassisted

Assisted

630

980

800

1250

1260

1960

BD FACSJazz 3 lasers: 488, 561, 640nm 6 colors + FSC, SSC

Unassisted

Assisted

630

980

800

1250

1260

1960

FACSAria III 4 lasers: 405, 488, 561, 633nm 11 colors + FSC, SSC

Unassisted

Assisted

630

980

800

1250

1260

1960

How to get access to the BFC

To get access to the BFC, the following steps have to be taken:

A) Apply for an account to the PPMS online booking system
This system it used to book analysis instruments and unassisted sorts*. Instructions on how to apply for a PPMS account can be found on our website.

B) Attend a “General introduction to the BFC” session (30 min)
This session will give information on how the facility is organized and to the rules and routines of the facility. After attending this introduction session you will get access to the facility with your key-card. You will also get added to the BFC-mailing list to receive information about the facility. The introduction sessions are arranged every 2-3 weeks.

C) Get driver’s license to run instruments
For analysis instruments a driver’s license can be obtained either by demonstrating previous experience on a particular instrument or by getting trained in the facility. For BD Aria sorters we periodically organise operator training courses, which consist of a theoretical and hands on 2-day course. Once this training is completed all users should practise and discuss their needs within the following 2-3 weeks so they get used to general routines and show competence to sort autonomously. As soon as the drive’s license is obtained, access to PPMS booking system will be permitted. Contact us for pricing and to schedule any training course or get a driver’s license.

*For assisted services (analysis or cell sorting), please send us an email

PPMS online booking system

The facility will use an online booking system to book analysis instruments and unassisted cell sorting.

*For assisted services (analysis and cell sorting), please send us an email to make a booking.

Click here to access the PPMS system

-If you have, now or in the past, logged onto PPMS, please send an email to saikiran.sedimbi@ki.se Indicate the instruments you have been trained on to get access.

-If you have a PPMS account, but have never used it, then choose “If you do not remember your password, you can follow these instructions” at the login page. You will receive a reset link to the email address registered in the system when you first applied. *Make sure to use your 6 letter KI login as username. (for example, John Smith, JONSMI).

If you don't have an account on PPMS, please follow the instructions in the PDF below to set one up.

PPMS Instructions for creating accounts

New users: Please send us an email to register for user training and access to the facility.

Personnel

Facility director

Senior forskningsspecialist

Saikiran Sedimbi

Enhet: FACS
E-post: Saikiran.Sedimbi@ki.se

Facility manager

Forskare

Maria Johansson

Telefon: 08-524 867 39
Enhet: Klas Kärre grupp
E-post: Maria.Johansson@ki.se

Research engineer

Forskningsingenjör

Juan Basile

Enhet: FACS
E-post: juan.basile@ki.se