Biomedicum Flow Cytometry Core Facility
The Biomedicum flow cytometry core facility (BFC) is a shared resource laboratory that will provide researchers with the capability of performing a wide range of flow cytometry experiments.
Location: Biomedicum, Quarter 3C (entrance level)
Instruments
The facility is equipped with the following instruments for both assisted and unassisted analysis and cell sorting experiments. Instrument configurations (excel file)
Analysis
| Instrument | Laser specification | Feasible parameters | KI users (including INDI) |
Other academic institutions PRICE (SEK) | Commercial users | |
|---|---|---|---|---|---|---|
| BD LSRII SORP | 4 lasers: 405, 488, 532, 640nm | 18 colors + FSC, SSC | Unassisted Assisted | 650 1080 |
670 1120 |
1060 1760 |
| BD FACS Verse | 3 lasers: 405, 488, 640 nm | 8 colors + FSC, SSC | Unassisted Assisted | 540 970 |
550 1000 |
880 1580 |
| FACS Calibur | 2 lasers: 488 & 633nm | 4 colors + FSC, SSC | Unassisted Assisted | 430 860 |
450 900 |
700 1400 |
| CyAn ADP | 3 lasers: 405, 488, 635nm | 9 colors + FSC, SSC | Unassisted Assisted | 540 970 |
550 1000 |
880 1580 |
| FACSCanto II | 3 lasers: 405, 488, 633nm | 8 colors + FSC, SSC | Unassisted Assisted | 540 970 |
550 1000 |
880 1580 |
Cell Sorting
| Instrument | Laser specification | Feasible parameters | KI users (including INDI) |
Other academic institutions PRICE (SEK) | Commercial users | |
|---|---|---|---|---|---|---|
| BD FACSAria Fusion | 4 lasers: 405, 488, 561, 640nm | 16 colors + FSC, SSC | Unassisted Assisted | 770 1200 |
800 1250 |
1260 1960 |
| BD FACSJazz | 3 lasers: 488, 561, 640nm | 6 colors + FSC, SSC | Unassisted Assisted | 770 1200 |
800 1250 |
1260 1960 |
| FACSAria III | 4 lasers: 405, 488, 561, 633nm | 11 colors + FSC, SSC | Unassisted Assisted | 770 1200 |
800 1250 |
1260 1960 |
How to get access to the BFC
To get access to the BFC, the following steps have to be taken:
A) Apply for an account in the iLab booking system. This system is used for all bookings and requests. Instructions on how to apply for a iLab account can be found below.
B) Attend a “General introduction to the BFC” session (30 min)
This session will give information on how the facility is organized and to the rules and routines of the facility. After attending this introduction session you will get access to the facility with your key-card. You will also get added to the BFC-mailing list to receive information about the facility. The introduction sessions are arranged every 2-3 weeks.
C) Get driver’s license to run instruments
For analysis instruments a driver’s license can be obtained either by demonstrating previous experience on a particular instrument or by getting trained in the facility. For BD Aria sorters we periodically organise operator training courses, which consist of a theoretical and hands on 2-day course. Once this training is completed all users should practise and discuss their needs within the following 2-3 weeks so they get used to general routines and show competence to sort autonomously. As soon as the drive’s license is obtained, access to iLabs booking system will be permitted. Contact us for pricing and to schedule any training course or get a driver’s license.
Online booking system
From August 18, 2020, the facility will switch to the iLab online booking system.
This system will be used for all bookings, service requests as well as introduction/training. Core iLab page
For instructions on how to create an account and to make reservations:
More information about implementation of iLab at KI.
Personnel
Maria Johansson
Facility manager