Sho Oasa

I completed my PhD in Life Science under the supervision of Prof. Masataka Kinjo at Laboratory of Molecular Cell Dynamics, Hokkaido University, Sapporo, Japan in 2016. I had a postdocotrall training at same laboratory in 2016-2017. I moved to Vladana Vukojevic and Lars Terenius's research group, Department of Clinical Neuroscience, Karolinska Institutet (KI) as a research associate for 2017-2019 financially supported by the Nakatani Foundation for Advancement of measuring technologies in biomedical engineering. Following the research associates, I was employed as the Postdoctoral fellowship in the same research group in KI for 2019-2021 financially supported by the postdoctoral funding by the Strategic Neuroscience. My career progresssed an Assistant Professor at 2021.

I'm a Biophysist with an expetise on fluorescence microscopy techniques including live-cell confocal microscopy imaigng, fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

Please feel free to contact me if you have any questions in the fluorescence microscopy.

Our group organizes the fluorescence microscopy core facility in Center for Molecular Medicine and PhD course in fluorescence microscopy.

Functional Fluorescence Microscopy Imaging (fFMI)

K8F2363: Functional Fluorescence Microscopy Imaging (fFMI) in Biomedical Research

I'm working on the sveral directions of researches in Karolinska Institutet.

1) Scanning-free confocal fluorescence microscopy towards massively parallel fluorescence correlation spectroscopy and fluorescence lifetime imaging microscopy (mpFCS/FLIM).

Fluorescence Correlation Spectroscopy (FCS) allows us to characterize the dynamic properties of fluorescently tagged proteins and its concentration in live cells. However, classical FCS based on a confocal microscopy focused laser in a selected position, being subjected to FCS analysis.

We are developping with Dr. Alexsandar Krmpot and Dr. Stanko Nikolic (Institute of Physics Belgrade, University of Belgrade, Serbia) the 2D imaging FCS instrument to record fluorescence intensity in different positions of cells at the same time. Parallel computing calculated large number of autocorrelation curves in FCS analysis to quantitatively characterize the spatial heterogeneity of protein dynamics and density/concentration in live cells. This instrument allows us to high-throughput analysis in in vitro samples as well as sptially resolved analysis in live cells.

2) Alcohol modulation on plasma membrane organization, opioid receptors dynamics/functionalities and opioid receptor antagonists' effect to the alocohol modulations.

(Constructing)

3) Development of small molecular decoys interfering with amyloid beta aggregation towards new medication to Alzheimer's disease

(Constructing)

4) Characterization of transcription factor dimerization inhibitor towards new medication for glioblastoma and medulloblastoma

(Constructing)

5) Glucocorticoid receptor (GR) transporting/dimerization kinetics coupling to the GR functionality.

(Constructing)