Rozbeh Jafari
About me
- Dr. Jafari obtained his PhD in biomedical sciences at the department of chemistry and biomedical sciences, Karlstad University, where he developed antibody fragments for cancer immunotherapy of solid tumors. As a postdoc with Prof. Pär Nordlund at the department of biochemistry and biophysics, Karolinska Institutet, he developed the Cellular Thermal Shift Assay (CETSA) and its proteome wide adaptation, Thermal Proteome Profiling (TPP) and studied target and off-target identification and interaction of oncology drugs. He was awarded a assistant professor position from Swedish Childhood Fund in 2016 and the starting grant from Swedish Research Council in 2017 to establish his own research group at the department of oncology-pathology at Karolinska Institutet to apply multi-omic analysis of leukemias. In Feb 2024 he was accepted as Associate Professor in cell and molecular biology at the department of onkologi-patologi.
Research
We are utilizing and integrating state-of-the-art omics methods to obtain detailed molecular phenotypes of childhood and adult acute lymphoblastic leukemia (ALL), aiming to identify precision medicine candidates for improved therapy. Building on our recent studies (Leo, I.R. et al., Nat Commun 13, 1691 (2022), PMID:35354797, Kurzawa, N. et al., Nat Chem Biol 19, 962–971 (2023), PMID:36941476), we are advancing the study and validation of precision medicine candidates, with a strong focus on their translational implementation.
A fundamental question we are currently addressing is how cancer genome aberrations influence the functional proteome by combining genomics with proteomics data, a field known as cancer proteogenomics. In addition to quantitative proteomics, we are enhancing our analysis by capturing the biophysical state of the proteome, regardless of its abundance, using thermal proteomics. This approach allows us to identify functional proteoforms as well as their association with drug sensitivity.
Furthermore, we are investigating the target landscape of current and emerging targeted therapeutics using orthogonal chemical proteomics approaches to understand their mechanisms of action and potential toxicities. Our research also focuses on molecular and cellular biology, aiming to translate our findings into clinical applications that can significantly improve patient outcomes.
Selected publications
- Article: NATURE CHEMICAL BIOLOGY. 2023;19(8):962-971
- Article: LEUKEMIA. 2023;37(1):61-71
- Article: NATURE COMMUNICATIONS. 2022;13(1):1691
- Article: THE FASEB JOURNAL. 2021;35(7):e21741
- Article: NATURE PROTOCOLS. 2014;9(9):2100-2122
- Article: SCIENCE. 2013;341(6141):84-87
Articles
- Article: CELL DEATH AND DISEASE. 2024;15(9):694
- Journal article: BLOOD CANCER DISCOVERY. 2024;5(2_Supplement):p30
- Article: NPJ PRECISION ONCOLOGY. 2024;8(1):38
- Article: SCIENTIFIC REPORTS. 2024;14(1):4000
- Journal article: CANCER RESEARCH. 2023;83(7):6612
- Article: NPJ PRECISION ONCOLOGY. 2023;7(1):32
- Article: BIOINFORMATICS ADVANCES. 2023;3(1):vbad143
- Article: NATURE COMMUNICATIONS. 2022;13(1):6226
- Article: CELL DEATH AND DISEASE. 2021;12(10):914
- Article: AMERICAN JOURNAL OF HEMATOLOGY. 2021;96(5):580-588
- Article: VIRUSES. 2020;12(12):E1423-1423
- Journal article: BLOOD. 2020;136:10-11
- Article: NATURE COMMUNICATIONS. 2019;10(1):1519
- Article: ANNALS OF ONCOLOGY. 2016;27(12):2275-2283
- Article: NATURE COMMUNICATIONS. 2016;7:11040
- Article: SCIENCE. 2014;346(6205):1255784
- Article: MICROBIAL CELL FACTORIES. 2011;10:34
- Article: JOURNAL OF IMMUNOLOGICAL METHODS. 2011;364(1-2):65-76
- Article: MABS. 2010;2(6):662-669
- Article: CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS. 2010;25(4):455-463
- Article: MOLECULAR IMMUNOLOGY. 2007;44(6):1075-1084
- Show more
All other publications
- Preprint: BIORXIV. 2023
- Preprint: RESEARCH SQUARE. 2023
- Conference publication: BLOOD. 2022;140:6299
- Preprint: BIORXIV. 2022
- Preprint: BIORXIV. 2022
- Preprint: RESEARCH SQUARE. 2021
- Preprint: BIORXIV. 2021
- Preprint: SSRN ELECTRONIC JOURNAL. 2020
- Meeting abstract: CANCER RESEARCH. 2016;76:4386
Grants
- Integrative multi-omics for mechanistic insights into acute lymphoblastic leukemia for precision therapiesSwedish Cancer Society: Senior Investigator Award1 July 2024 - 30 June 2027
- Integrative multi-omics for mechanistic insights into acute lymphoblastic leukemia for precision therapiesThe Swedish Cancer Society1 January 2024 - 31 December 2026
- Swedish Cancer Society1 January 2024
- Swedish Research Council1 January 2018 - 31 December 2021
- Predictive therapeutic and resistance biomarkers in childhood leukemia using chemical proteomicsThe Swedish Childhood Cancer Fund1 January 2017 - 31 December 2019
- Predictive therapeutic and resistance biomarkers in childhood leukemia using chemical proteomicsThe Swedish Childhood Cancer Fund1 September 2016 - 31 August 2021
Employments
- Principal Researcher, Department of Oncology-Pathology, Karolinska Institutet, 2024-
Degrees and Education
- Docent, Department of Oncology-Pathology, Karolinska Institutet, 2024
- PhD, Department of Chemistry and Biomedical Sciences, Karlstad University, 2010