Nuno Rufino de Sousa

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About me

Research on aerosol transmissible diseases and aeromicrobiology.
Operations manager of BSL3 Core Facility at Biomedicum.

Research

My research is done on the new field of aeromicrobiology focusing on aerosol
transmissible diseases, more specifically Mycobacterium tuberculosis (Mtb).
This is further divided into two projects, the study of survivability and
fitness of Mtb in aerosols and the study of Mtb aerosols infection dynamics.
In our lab we apply the methods we are developing to study the
aeromicrobiology of Mtb to other aerosol transmissible diseases such as
SARS-CoV-2 and we perform clinical and environmental aerosol studies were we
collect air-samples to study disease propagation and aerobiome respectively.

Articles

Article: INFECTIOUS DISEASES. 2024;56(11):991-999
Detection of active SARS-CoV-2 in cough aerosols from COVID-19 patients
de Sousa NR; Margerie L; Steponaviciute L; Roux J; Kinahan MW; Olsson D; Asgeirsson H; Udekwu KI; Rothfuchs AG
Journal article: BIOSENSORS AND BIOELECTRONICS: X. 2024;19:100488
A fieldable process for sensitive detection of airborne viruses via electrophoresis-based RNA enrichment
Du H; Bruno S; Overholt KJ; Palacios S; Huang H-H; Barajas C; Gross B; Lee C; Evile HK; de Sousa NR; Rothfuchs AG; Del Vecchio D
Article: MOLECULAR PSYCHIATRY. 2022;27(10):3939-3950
SARS-CoV-2 promotes microglial synapse elimination in human brain organoids
Samudyata; Oliveira AO; Malwade S; de Sousa NR; Goparaju SK; Gracias J; Orhan F; Steponaviciute L; Schalling M; Sheridan SD; Perlis RH; Rothfuchs AG; Sellgren CM
Article: INDOOR AIR. 2022;32(3):e13023
Detection and isolation of airborne SARS-CoV-2 in a hospital setting
de Sousa NR; Steponaviciute L; Margerie L; Nissen K; Kjellin M; Reinius B; Salaneck E; Udekwu KI; Rothfuchs AG
Article: CELL. 2021;184(13):3376-3393.e17
A global metagenomic map of urban microbiomes and antimicrobial resistance
Danko D; Bezdan D; Afshin EE; Ahsanuddin S; Bhattacharya C; Butler DJ; Chng KR; Donnellan D; Hecht J; Jackson K; Kuchin K; Karasikov M; Lyons A; Mak L; Meleshko D; Mustafa H; Mutai B; Neches RY; Ng A; Nikolayeva O; Nikolayeva T; Png E; Ryon KA; Sanchez JL; Shaaban H; Sierra MA; Thomas D; Young B; Abudayyeh OO; Alicea J; Bhattacharyya M; Blekhman R; Castro-Nallar E; Canas AM; Chatziefthimiou AD; Crawford RW; De Filippis F; Deng Y; Desnues C; Dias-Neto E; Dybwad M; Elhaik E; Ercolini D; Frolova A; Gankin D; Gootenberg JS; Graf AB; Green DC; Hajirasouliha I; Hastings JJA; Hernandez M; Iraola G; Jang S; Kahles A; Kelly FJ; Knights K; Kyrpides NC; Labaj PP; Lee PKH; Leung MHY; Ljungdahl PO; Mason-Buck G; McGrath K; Meydan C; Mongodin EF; Moraes MO; Nagarajan N; Nieto-Caballero M; Noushmehr H; Oliveira M; Ossowski S; Osuolale OO; Ozcan O; Paez-Espino D; Rascovan N; Richard H; Ratsch G; Schriml LM; Semmler T; Sezerman OU; Shi L; Shi T; Siam R; Song LH; Suzuki H; Court DS; Tighe SW; Tong X; Udekwu KI; Ugalde JA; Valentine B; Vassilev DI; Vayndorf EM; Velavan TP; Wu J; Zambrano MM; Zhu J; Zhu S; Mason CE
Article: ANNALS OF WORK EXPOSURES AND HEALTH. 2020;64(8):852-865
Operative and Technical Modifications to the Coriolis® μ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling
de Sousa NR; Shen L; Silcott D; Call CJ; Rothfuchs AG
Article: NATURE COMMUNICATIONS. 2020;11(1):4812
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
Smyrlaki I; Ekman M; Lentini A; de Sousa NR; Papanicolaou N; Vondracek M; Aarum J; Safari H; Muradrasoli S; Rothfuchs AG; Albert J; Hoegberg B; Reinius B
Article: TUBERCULOSIS. 2020;120:101896
A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols
de Sousa NR; Sandstrom N; Shen L; Hakansson K; Vezozzo R; Udekwu KI; Croda J; Rothfuchs AG

All other publications

Letter: INDOOR AIR. 2022;32(11):e13169
Plaque-forming units from air samples: Letter to Editor. Re: Jefferson et al., Indoor Air, 2022
Rufino de Sousa N; Steponaviciute L; Margerie L; Nissen K; Kjellin M; Reinius B; Salaneck E; Udekwu KI; Rothfuchs AG
Corrigendum: INDOOR AIR. 2022;32(8):e13085
Detection and isolation of airborne SARS-CoV-2 in a hospital setting (vol 32, e13023, 2022)
Rufino de Sousa N; Steponaviciute L; Margerie L; Nissen K; Kjellin M; Reinius B; Salaneck E; Udekwu KI; Rothfuchs AG
Preprint: RESEARCH SQUARE. 2021
SARS-CoV-2 Neurotropism and Single Cell Responses in Brain Organoids Containing Innately Developing Microglia
Samudyata S; Oliveira A; Malwade S; de Sousa NR; Goparaju S; Orhan F; Steponaviciute L; Sheridan S; Perlis R; Rothfuchs A; Sellgren C
Preprint: BIORXIV. 2021
SARS-CoV-2 promotes microglial synapse elimination in human brain organoids
Samudyata; Oliveira AO; Malwade S; de Sousa NR; Goparaju S; Lekander JG; Orhan F; Steponaviciute L; Schalling M; Sheridan S; Perlis R; Rothfuchs A; Sellgren C
Preprint: MEDRXIV. 2020
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
Smyrlaki I; Ekman M; Lentini A; Sousa NRD; Papanicolaou N; Vondracek M; Aarum J; Safari H; Muradrasoli S; Rothfuchs AG; Albert J; Högberg B; Reinius B

Employments

Laboratory Technician, Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 2020-
Phd Student, Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 2022-2025

About me

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