Nicola Crosetto
Principal Researcher
E-mail: nicola.crosetto@ki.se
Telephone: +46852485258
Visiting address: Tomtebodavägen 23A (Gamma4), 17165 Solna
Postal address: C1 Mikrobiologi, tumör- och cellbiologi, C1 SciLife Bienko, 171 77 Stockholm
About me
- I graduated in Medicine at the University of Pavia, Italy in 2003 and
specialized in Medical Oncology at the University of Turin, Italy in 2007.
From 2008 until 2010, I worked in Ivan Dikic’s lab at the Goethe University
in Frankfurt, Germany and in 2011 I obtained a PhD in Bioengineering and
Bioinformatics at the University of Pavia, Italy. From 2011 until 2014 I was
a postdoc in Alexander van Oudenaarden’s lab at MIT, where I worked on
developing novel single-molecule microscopy methods. Since 2015, I have been
leading my own research group at Karolinska Institutet, with a major focus on
developing microscopy- and sequencing-based methods to investigate genome
fragility and genome organization in healthy and pathological cell states.
Since 2022 I also lead the Genomic Technologies Development Unit in the
Genomics Research Centre at Human Technopole in Milan, Italy.
Over the years, I have developed numerous molecular methods, including the
first method for genome-wide mapping of DNA double-strand breaks (BLESS, /Nat
Meth/ 2013) and its downstream improvements (BLISS, /Nat Commun/ 2017
sBLISS, /Nat Prot/ 2020), as well as a variety of sequencing- (CUTseq, /Nat
Commun/ 2019- GPSeq, /Nat Biotechnol/ 2020
- COVseq, /Nat Commun/ 20221) and
microscopy-based methods (HD-FISH, /Nat Meth/ 2013 - FuseFISH, /Cell Rep/
2014 - RollFISH, /Comm Bio/ 2018
- iFISH, /Nat Commun/ 2019
- FRET-FISH, /Nat
Commun/ 2022) for studying various aspects of genome organization and
fragility.
Research
- Our main research interest is in understanding how the nucleus of eukaryotic
cells is spatially organized, and how the three-dimensional (3D) architecture
of the genome influences gene expression regulation and genome integrity.
We are particularly interested in understanding the role of 3D genome
architecture and genome fragility in the context of cancer, aiming at
developing improved diagnostic and patient stratification approaches.
Towards these goals, we combine high-end microscopy methods, next-generation
sequencing techniques, and advanced computational tools, to assess
chromosomal contacts, chromatin modifications, DNA breaks, chromosomal
rearrangements and gene expression levels in cultured cells and
patient-derived tissue samples.
Articles
- Article: NATURE COMMUNICATIONS. 2024;15(1):3475
- Article: NATURE COMMUNICATIONS. 2024;15(1):1768
- Article: SCIENCE ADVANCES. 2023;9(45):eadi2095
- Article: BIOLOGY OPEN. 2023;12(10):bio060113
- Article: CURRENT OPINION IN GENETICS & DEVELOPMENT. 2023;78:102020
- Article: JTO CLINICAL AND RESEARCH REPORTS. 2022;3(12):100435
- Article: NATURE COMMUNICATIONS. 2022;13(1):6680
- Article: BMC CANCER. 2022;22(1):1001
- Article: GENOME MEDICINE. 2022;14(1):98
- Article: SCIENTIFIC DATA. 2022;9(1):400
- Article: SCIENTIFIC DATA. 2022;9(1):47
- Article: CURRENT OPINION IN CELL BIOLOGY. 2022;74:7-12
- Article: MOLECULAR CELL. 2021;81(24):5007-5024.e9
- Article: MOLECULAR CELL. 2021;81(23):4907-4923.e8
- Article: NATURE COMMUNICATIONS. 2021;12(1):3903
- Article: VACCINES. 2021;9(3):235
- Article: METHODS IN MOLECULAR BIOLOGY. 2021;2162:261-281
- Article: FRONTIERS IN ONCOLOGY. 2021;11:700568
- Article: NATURE PROTOCOLS. 2020;15(12):3894-3941
- Article: NATURE BIOTECHNOLOGY. 2020;38(10):1184-1193
- Article: NATURE MEDICINE. 2020;26(7):1063-1069
- Article: NATURE COMMUNICATIONS. 2019;10(1):4732
- Article: CELL REPORTS. 2019;29(3):560-572.e4
- Article: MOLECULAR CELL. 2019;75(2):267-283.e12
- Article: NATURE GENETICS. 2019;51(6):1011-1023
- Article: NATURE COMMUNICATIONS. 2019;10(1):1636
- Article: GENOME BIOLOGY. 2019;20(1):28
- Article: CELL. 2018;173(4):879-893.e13
- Article: METHODS IN MOLECULAR BIOLOGY. 2018;1672:167-194
- Article: METHODS IN MOLECULAR BIOLOGY. 2018;1766:303-333
- Article: COMMUNICATIONS BIOLOGY. 2018;1:209
- Article: COMMUNICATIONS BIOLOGY. 2018;1:181
- Article: NATURE COMMUNICATIONS. 2017;8(1):374
- Article: NATURE COMMUNICATIONS. 2017;8(1):228
- Article: NATURE BIOTECHNOLOGY. 2017;35(8):789-792
- Article: NATURE COMMUNICATIONS. 2017;8:15656
- Article: NATURE COMMUNICATIONS. 2017;8:15058
- Article: BLOOD. 2017;129(18):2479-2492
- Article: ONCOTARGET. 2017;8(12):18680-18698
- Article: NATURE BIOTECHNOLOGY. 2016;34(8):852-856
- Article: NATURE MEDICINE. 2016;22(3):270-277
- Article: BLOOD CANCER JOURNAL. 2014;4(10):e249
- Article: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE. 2014;8(5):396-406
- Article: CELL REPORTS. 2014;6(1):18-23
- Article: NATURE METHODS. 2013;10(4):361-365
- Article: NATURE METHODS. 2013;10(2):122-124
- Article: ONCOGENE. 2012;31(43):4599-4608
- Article: CARDIOVASCULAR TOXICOLOGY. 2012;12(2):135-142
- Article: JOURNAL OF PROTEOME RESEARCH. 2011;10(5):2140-2153
- Article: ANNALS OF ONCOLOGY. 2011;22(3):712-717
- Article: CELL. 2010;143(5):677-681
- Article: MOLECULAR CELL. 2010;37(3):396-407
- Article: INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 2010;54(11-12):1649-1657
- Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2008;283(50):35173-35185
- Article: BLOOD. 2007;110(3):1004-1012
- Article: EUROPEAN JOURNAL OF CANCER. 2006;42(18):3095-3102
- Article: NATURE CELL BIOLOGY. 2006;8(2):163-169
- Article: SCIENCE. 2005;310(5755):1821-1824
- Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2004;279(31):32786-32795
- Article: LEUKEMIA. 2003;17(11):2107-2112
- Article: BRITISH JOURNAL OF HAEMATOLOGY. 2002;118(2):545-549
- Show more
All other publications
- Corrigendum: NATURE COMMUNICATIONS. 2024;15(1):3390
- Editorial comment: MOLECULAR CELL. 2024;84(1):12-13
- Editorial comment: NATURE METHODS. 2023;20(5):641-642
- Review: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY. 2023;11:1303862
- Review: TRENDS IN GENETICS. 2022;38(10):1062-1075
- Editorial comment: TUMORI JOURNAL. 2020;106(5):427-431
- Review: FRONTIERS IN ONCOLOGY. 2020;10:569202
- Review: FRONTIERS IN GENETICS. 2020;11:33
- Review: GENES. 2018;9(12):E632-632
- Corrigendum: METHODS IN MOLECULAR BIOLOGY. 2018;1766:E1
- Review: NATURE REVIEWS GENETICS. 2015;16(1):57-66
- Editorial comment: INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 2008;52(7):801-809
- Other: EUROPEAN JOURNAL OF HISTOCHEMISTRY. 2008;52(1):79-81
- Review: MOLECULAR CANCER RESEARCH. 2006;4(12):899-904
- Review: FEBS LETTERS. 2005;579(15):3231-3238