Nicola Crosetto

Nicola Crosetto

Principal Researcher
Telephone: +46852485258
Visiting address: Tomtebodavägen 23A (Gamma4), 17165 Solna
Postal address: C1 Mikrobiologi, tumör- och cellbiologi, C1 SciLife Bienko, 171 77 Stockholm

About me

  • I graduated in Medicine at the University of Pavia, Italy in 2003 and
    specialized in Medical Oncology at the University of Turin, Italy in 2007.
    From 2008 until 2010, I worked in Ivan Dikic’s lab at the Goethe University
    in Frankfurt, Germany and in 2011 I obtained a PhD in Bioengineering and
    Bioinformatics at the University of Pavia, Italy. From 2011 until 2014 I was
    a postdoc in Alexander van Oudenaarden’s lab at MIT, where I worked on
    developing novel single-molecule microscopy methods. Since 2015, I have been
    leading my own research group at Karolinska Institutet, with a major focus on
    developing microscopy- and sequencing-based methods to investigate genome
    fragility and genome organization in healthy and pathological cell states.
    Since 2022 I also lead the Genomic Technologies Development Unit in the
    Genomics Research Centre at Human Technopole in Milan, Italy.
    Over the years, I have developed numerous molecular methods, including the
    first method for genome-wide mapping of DNA double-strand breaks (BLESS, /Nat
    Meth/ 2013) and its downstream improvements (BLISS, /Nat Commun/ 2017

  • sBLISS, /Nat Prot/ 2020), as well as a variety of sequencing- (CUTseq, /Nat
    Commun/ 2019
  • GPSeq, /Nat Biotechnol/ 2020
  • COVseq, /Nat Commun/ 20221) and
    microscopy-based methods (HD-FISH, /Nat Meth/ 2013
  • FuseFISH, /Cell Rep/
  • RollFISH, /Comm Bio/ 2018
  • iFISH, /Nat Commun/ 2019
  • FRET-FISH, /Nat
    Commun/ 2022) for studying various aspects of genome organization and


  • Our main research interest is in understanding how the nucleus of eukaryotic
    cells is spatially organized, and how the three-dimensional (3D) architecture
    of the genome influences gene expression regulation and genome integrity.
    We are particularly interested in understanding the role of 3D genome
    architecture and genome fragility in the context of cancer, aiming at
    developing improved diagnostic and patient stratification approaches.
    Towards these goals, we combine high-end microscopy methods, next-generation
    sequencing techniques, and advanced computational tools, to assess
    chromosomal contacts, chromatin modifications, DNA breaks, chromosomal
    rearrangements and gene expression levels in cultured cells and
    patient-derived tissue samples.


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