Charlotte Rolny

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About me

Leading a research team that focuses on tumor immunology specifically to understand the underlying mechanisms that regulate the pro-tumoral phenotype of immune cells denoted Tumor-Associated Macrophages (TAMs). By employing state-of-art mouse models, cutting edge molecular biology techniques and patient samples, we have deciphered several mechanisms whereby pro-tumoral TAM phenotypes are regulated. By targeting these controlled pathways, pro-tumoral TAMs can be reprogrammed into an anti-tumoral phenotype leading to activation of cytotoxic immune cells and hampering tumor growth and metastatic dissemination. We work closely to clinicians assuring a bench-to-bedside link. Topics that we address:
i) Identifying novel targets that govern tumor-associated macrophage pro-tumoral functions by single-cell sequencing in, genome wide sequencing of total and ribosome- bound RNA, and proteomics.
ii) Identifying novel tumor microenvironment biomarkers in patient samples including TAM phenotypes and their spatial localization.
iii) Regulation of metastatic dissemination via the hematogenous route to secondary organs

Articles

Article: CELL REPORTS. 2023;42(12):113507
Report VEGF-C-expressing TAMs rewire the metastatic fate of breast cancer cells
Banerjee K; Kerzel T; Bekkhus T; Ferreira SDS; Wallmann T; Wallerius M; Landwehr L-S; Agardy DA; Schauer N; Malmerfeldt A; Bergh J; Bartish M; Hartman J; Ostman A; Squadrito ML; Rolny C
Article: JOURNAL FOR IMMUNOTHERAPY OF CANCER. 2022;10(10):e005308
Tumor MHC class I expression alters cancer-associated myelopoiesis driven by host NK cells
Neo SY; Jing X; Tong L; Tong D; Gao J; Chen Z; De Los Santos MC; Burduli N; Ferreira SDS; Wagner AK; Alici E; Rolny C; Cao Y; Lundqvist A
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2021;118(10):e2017089118
TAp73 represses NF-κB-mediated recruitment of tumor-associated macrophages in breast cancer
Wolfsberger J; Sakil HAM; Zhou L; van Bree N; Baldisseri E; Ferreira SDS; Zubillaga V; Stantic M; Fritz N; Hartman J; Rolny C; Wilhelm MT
Article: GYNECOLOGIC ONCOLOGY. 2020;159(3):860-868
High density of stroma-localized CD11c-positive macrophages is associated with longer overall survival in high-grade serous ovarian cancer
Corvigno S; Mezheyeuski A; De La Fuente LM; Westbom-Freme S; Carlson JW; Fernebro J; Avall-Lundqvist E; Kannisto P; Hedenfalk I; Malander S; Rolny C; Dahlstrand H; Ostman A
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2020;117(44):27556-27565
MNK2 governs the macrophage antiinflammatory phenotype
Bartish M; Tong D; Pan Y; Wallerius M; Liu H; Ristau J; Ferreira SDS; Wallmann T; van Hoef V; Masvidal L; Kerzel T; Joly A-L; Goncalves C; Preston SEJ; Ebrahimian T; Seitz C; Bergh J; Pietras K; Lehoux S; Naldini L; Andersson J; Squadrito ML; del RSV; Larsson O; Rolny C
Article: CANCERS. 2019;11(10):E1479-1479
STAT3 Activity Promotes Programmed-Death Ligand 1 Expression and Suppresses Immune Responses in Breast Cancer
Zerdes I; Wallerius M; Sifakis EG; Wallmann T; Betts S; Bartish M; Tsesmetzis N; Tobin NP; Coucoravas C; Bergh J; Rassidakis GZ; Rolny C; Foukakis T
Article: AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE. 2019;200(3):348-358
Distinct Cancer-Promoting Stromal Gene Expression Depending on Lung Function
Sandri BJ; Masvidal L; Murie C; Bartish M; Avdulov S; Higgins L; Markowski T; Peterson M; Bergh J; Yang P; Rolny C; Limper AH; Griffin TJ; Bitterman PB; Wendt CH; Larsson O
Article: CLINICAL CANCER RESEARCH. 2019;25(12):3702-3717
A Novel ACKR2-Dependent Role of Fibroblast-Derived CXCL14 in Epithelial-to-Mesenchymal Transition and Metastasis of Breast Cancer
Sjoberg E; Meyrath M; Milde L; Herrera M; Lovrot J; Hagerstrand D; Frings O; Bartish M; Rolny C; Sonnhammer E; Andy CE; Augsten M; Ostman A
Journal article: PANCREATOLOGY. 2019;19:s28-s29
Smad7 is required for normal macrophage function in experimental chronic pancreatitis
Li X; Wallmann T; Nania S; Hornung L; Kleiter I; Löhr J-M; Rolny C; Heuchel R
Journal article: ANNALS OF THE AMERICAN THORACIC SOCIETY. 2018;15(Supplement_4):s290-s291
Chronic Obstructive Pulmonary Disease Phenotype Dictates Cancer-Promoting Stromal Gene Expression Programs
Wendt CH; Sandri BJ; Masvidal L; Murie C; Bartish M; Avdulov S; Higgins L; Markowski T; Peterson M; Bergh J; Yang P; Rolny C; Limper AH; Griffin TJ; Bitterman PB; Larsson O
Article: ISCIENCE. 2018;9:71-83
Microglia Induce PDGFRB Expression in Glioma Cells to Enhance Their Migratory Capacity
Wallmann T; Zhang X-M; Wallerius M; Bolin S; Joly A-L; Sobocki C; Leiss L; Jiang Y; Bergh J; Holland EC; Enger PO; Andersson J; Swartling FJ; Miletic H; Uhrbom L; Harris RA; Rolny C
Article: CANCER IMMUNOLOGY RESEARCH. 2018;6(11):1417-1425
Cripto-1 Plasmid DNA Vaccination Targets Metastasis and Cancer Stem Cells in Murine Mammary Carcinoma
Witt K; Ligtenberg MA; Conti L; Lanzardo S; Ruiu R; Wallmann T; Tufvesson-Stiller H; Chambers BJ; Rolny C; Lladser A; Lundqvist A; Cavallo F; Kiessling R
Article: ONCOIMMUNOLOGY. 2017;6(8):e1338238
Zoledronic acid inhibits NFAT and IL-2 signaling pathways in regulatory T cells and diminishes their suppressive function in patients with metastatic cancer
Sarhan D; Leijonhufvud C; Murray S; Witt K; Seitz C; Wallerius M; Xie H; Ullen A; Harmenberg U; Lidbrink E; Rolny C; Andersson J; Lundqvist A
Article: CANCER RESEARCH. 2016;76(11):3166-3178
Guidance Molecule SEMA3A Restricts Tumor Growth by Differentially Regulating the Proliferation of Tumor-Associated Macrophages
Wallerius M; Wallmann T; Bartish M; Ostling J; Mezheyeuski A; Tobin NP; Nygren E; Pangigadde P; Pellegrini P; Squadrito ML; Ponten F; Hartman J; Bergh J; DeMilito A; De Palma M; Ostman A; Andersson J; Rolny C
Article: CELL REPORTS. 2016;15(9):2000-2011
Reprogramming Tumor-Associated Macrophages by Antibody Targeting Inhibits Cancer Progression and Metastasis
Georgoudaki A-M; Prokopec KE; Boura VF; Hellqvist E; Sohn S; Ostling J; Dahan R; Harris RA; Rantalainen M; Klevebring D; Sund M; Brage SE; Fuxe J; Rolny C; Li F; Ravetch JV; Karlsson MCI
Article: SCIENCE. 2015;347(6226):1138-1142
Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq
Zeisel A; Munoz-Manchado AB; Codeluppi S; Lonnerberg P; La Manno G; Jureus A; Marques S; Munguba H; He L; Betsholtz C; Rolny C; Castelo-Branco G; Hjerling-Leffler J; Linnarsson S
Article: EUROPEAN JOURNAL OF IMMUNOLOGY. 2013;43(1):249-257
Activated monocytes augment TRAIL-mediated cytotoxicity by human NK cells through release of IFN-γ.
Sarhan D; D'Arcy P; Wennerberg E; Lidén M; Hu J; Winqvist O; Rolny C; Lundqvist A
Article: CANCER RESEARCH. 2012;72(8):1953-1963
Genetic Deficiency in Plasma Protein HRG Enhances Tumor Growth and Metastasis by Exacerbating Immune Escape and Vessel Abnormalization
Tugues S; Honjo S; Konig C; Noguer O; Hedlund M; Botling J; Deschoemaeker S; Wenes M; Rolny C; Jahnen-Dechent W; Mazzone M; Claesson-Welsh L
Journal article: JOURNAL OF CLINICAL INVESTIGATION. 2011;121(7):2945
Sema3E–Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice
Casazza A; Finisguerra V; Capparuccia L; Camperi A; Swiercz JM; Rizzolio S; Rolny C; Christensen C; Bertotti A; Sarotto I; Risio M; Trusolino L; Weitz J; Schneider M; Mazzone M; Comoglio PM; Tamagnone L
Journal article: CANCER RESEARCH. 2011;71(8_Supplement):3268
Abstract 3268: Targeting Semaphorin signaling to inhibit tumor growth and metastatic spreading
Casazza A; Kigel B; Rolny C; Neufeld G; Tamagnone L
Article: ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY. 2011;31(4):741-749
Systemic and Targeted Delivery of Semaphorin 3A Inhibits Tumor Angiogenesis and Progression in Mouse Tumor Models
Casazza A; Fu X; Johansson I; Capparuccia L; Andersson F; Giustacchini A; Squadrito ML; Venneri MA; Mazzone M; Larsson E; Carmeliet P; De Palma M; Naldini L; Tamagnone L; Rolny C
Article: CANCER CELL. 2011;19(1):31-44
HRG Inhibits Tumor Growth and Metastasis by Inducing Macrophage Polarization and Vessel Normalization through Downregulation of PIGF
Rolny C; Mazzone M; Tugues S; Laoui D; Johansson I; Coulon C; Squadrito ML; Segura I; Li X; Knevels E; Costa S; Vinckier S; Dresselaer T; Akerud P; De Mol M; Salomaki H; Phillipson M; Wyns S; Larsson E; Buysschaert I; Botling J; Himmelreich U; Van Ginderachter JA; De Palma M; Dewerchin M; Claesson-Welsh L; Carmeliet P
Article: DIABETES. 2010;59(10):2569-2578
Clinical and Experimental Pancreatic Islet Transplantation to Striated Muscle Establishment of a Vascular System Similar to That in Native Islets
Christoffersson G; Henriksnas J; Johansson L; Rolny C; Ahlstrom H; Caballero-Corbalan J; Segersvard R; Permert J; Korsgren O; Carlsson P-O; Phillipson M
Article: BLOOD. 2010;116(11):1924-1931
A chemotactic gradient sequestered on endothelial heparan sulfate induces directional intraluminal crawling of neutrophils
Massena S; Christoffersson G; Hjertstrom E; Zcharia E; Vlodavsky I; Ausmees N; Rolny C; Li J-P; Phillipson M
Article: JOURNAL OF CLINICAL INVESTIGATION. 2010;120(8):2684-2698
Sema3E-Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice
Casazza A; Finisguerra V; Capparuccia L; Camperi A; Swiercz JM; Rizzolio S; Rolny C; Christensen C; Bertotti A; Sarotto I; Risio M; Trusolino L; Weitz J; Schneider M; Mazzone M; Comoglio PM; Tamagnone L
Article: CELL. 2010;141(1):178-190
Further Pharmacological and Genetic Evidence for the Efficacy of PlGF Inhibition in Cancer and Eye Disease
Van de Veire S; Stalmans I; Heindryckx F; Oura H; Tijeras-Raballand A; Schmidt T; Loges S; Albrecht I; Jonckx B; Vinckier S; Van Steenkiste C; Tugues S; Rolny C; De Mol M; Dettori D; Hainaud P; Coenegrachts L; Contreres J-O; Van Bergen T; Cuervo H; Xiao W-H; Le Henaff C; Buysschaert I; Masouleh BK; Geerts A; Schomber T; Bonnin P; Lambert V; Haustraete J; Zacchigna S; Rakic J-M; Jimenez W; Noel A; Giacca M; Colle I; Foidart J-M; Tobelem G; Morales-Ruiz M; Vilar J; Maxwell P; Vinores SA; Carmeliet G; Dewerchin M; Claesson-Welsh L; Dupuy E; Van Vlierberghe H; Christofori G; Mazzone M; Detmar M; Collen D; Carmeliet P
Article: JOURNAL OF EXPERIMENTAL MEDICINE. 2008;205(5):1155-1171
The tumor suppressor semaphorin 3B triggers a prometastatic program mediated by interleukin 8 and the tumor microenvironment
Rolny C; Capparuccia L; Casazza A; Mazzone M; Vallario A; Cignetti A; Medico E; Carmeliet P; Comoglio PM; Tamagnone L
Article: BLOOD. 2006;108(6):1877-1886
Platelet-derived growth factor receptor-β promotes early endothelial cell differentiation
Rolny C; Nilsson I; Magnusson P; Armulik A; Jakobsson L; Wentzel P; Lindblom P; Norlin J; Betsholtz C; Heuchel R; Welsh M; Claesson-Welsh L
Article: EXPERIMENTAL CELL RESEARCH. 2005;308(2):381-393
Shb promotes blood vessel formation in embryoid bodies by augmenting vascular endothelial growth factor receptor-2 and platelet-derived growth factor receptor-β signaling
Rolny C; Lu LG; Ågren N; Nilsson I; Roe C; Webb GC; Welsh M
Article: FASEB JOURNAL. 2004;18(13):1507-1515
Vascular endothelial growth factor receptor-3 in hypoxia-induced vascular development
Nilsson I; Rolny C; Wu Y; Pytowski B; Hicklin D; Alitalo K; Claesson-Welsh L; Wennström S
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2004;279(21):22267-22275
The adaptor protein shb binds to tyrosine 1175 in vascular endothelial growth factor (VEGF) receptor-2 and regulates VEGF-dependent cellular migration
Holmqvist K; Cross MJ; Rolny C; Hägerkvist R; Rahimi N; Matsumoto T; Claesson-Welsh L; Welsh M
Article: JOURNAL OF CELL SCIENCE. 2004;117(8):1513-1523
Deregulation of Flk-1/vascular endothelial growth factor receptor-2 in fibroblast growth factor receptor-1-deficient vascular stem cell development
Magnusson P; Rolny C; Jakobsson L; Wikner C; Wu Y; Hicklin DJ; Claesson-Welsh L
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2002;277(22):19315-19321
Heparin amplifies platelet-derived growth factor (PDGF)-BB-induced PDGF α-receptor but not PDGF β-receptor tyrosine phosphorylation in heparan sulfate-deficient cells -: Effects on signal transduction and biological responses
Rolny C; Spillmann D; Lindahl U; Claesson-Welsh L
Article: INTERNATIONAL JOURNAL OF ONCOLOGY. 1998;12(2):309-313
Expression of human endogenous retrovirus ERV3 (HERV-R) mRNA in normal and neoplastic tissues
Andersson AC; Svensson AC; Rolny C; Andersson G; Larsson E
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All other publications

Review: NATURE REVIEWS CANCER. 2023;23(6):408-425
The role of eIF4F-driven mRNA translation in regulating the tumour microenvironment
Bartish M; Abraham MJ; Goncalves C; Larsson O; Rolny C; Del Rincon SV
Meeting abstract: CANCER RESEARCH. 2023;83(5):p2-20-08-p2-20-08
Expression of ACVRL1 in a subset of recruited tumor-associated macrophages drives resistance to PD-1 therapy in human breast cancer
Talkhoncheh MS; Sjolund J; Balbas PB; Kurzejamska E; Larsson S; Cordero E; Oudenaarden CR; Pantaleo J; Jonsson G; Rolny C; Bocci M; Pietras K
Preprint: BIORXIV. 2022
VEGF-C expression in TAMs rewires the metastatic fate of breast cancer cells
Banerjee K; Kerzel T; Bekkhus T; de Souza Ferreira S; Wallmann T; Wallerius M; Landwehr L-S; Agardy DA; Schauer N; Malmerfeldt A; Bergh J; Bartish M; Hartman J; Östman A; Squadrito ML; Rolny C
Corrigendum: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2021;118(46):e2117333118
TAp73 represses NF-κB-mediated recruitment of tumor-associated macrophages in breast cancer (vol 118, e2017089118, 2021)
Wolfsberger J; Sakil HAM; Zhou L; van Bree N; Baldisseri E; Ferreira SDS; Zubillaga V; Stantic M; Fritz N; Hartman J; Rolny C; Wilhelm MT
Meeting abstract: CANCER IMMUNOLOGY RESEARCH. 2018;6(9):a77
Cripto-1 vaccination elicits protective immune response to metastatic breast cancer and breast cancer stem cells
Witt K; Ligtenberg MA; Conti L; Lanzardo S; Ruiu R; Tufvesson-Stiller H; Ostling J; Rolny C; Lladser A; Lundqvist A; Cavallo F; Kiessling R
Meeting abstract: JOURNAL OF CLINICAL ONCOLOGY. 2017;35:e23088
Regulation of PD-L1 in breast cancer
Zerdes I; Tsesmetzis N; Lovrot J; Rolny C; Bergh JCS; Rassidakis G; Foukakis T
Conference publication: SCANDINAVIAN JOURNAL OF IMMUNOLOGY. 2017;86(4):312-313
Differential translational regulation dictates the TAM phenotype during the course of tumor growth
Bartish M; Wallerius M; Wallmann T; Sanz LM; van Hoef V; Joly A-L; Andersson J; Larsson O; Rolny C
Conference publication: SCANDINAVIAN JOURNAL OF IMMUNOLOGY. 2017;86(4):315
Polysomal profiling reveals differential translational regulation in TAMs during tumor growth
Bartish M; Wallerius M; Wallmann T; Sanz LM; Van Hoef V; Joly A-L; Andersson J; Rolny C; Larsson O
Conference publication: SCANDINAVIAN JOURNAL OF IMMUNOLOGY. 2017;86(4):314-315
Semaphorin3A re-educates myeloid derived suppressor cells toward a pro-inflammatory phenotype
Wallerius M; Bartish M; Wallmann T; Andresson J; Rolny C
Conference publication: SCANDINAVIAN JOURNAL OF IMMUNOLOGY. 2017;86(4):315-316
Macrophage-derived Vascular Endothelial Growth Factor C re-directs hematogenous metastatic dissemination by normalizing the tumor vasculature
Wallmann T; Wallerius M; Landwehr L; Agardy D; Bartish M; Squadrito M; De Palma M; Rolny C
Conference publication: SCANDINAVIAN JOURNAL OF IMMUNOLOGY. 2017;86(4):334
Cripto-1 vaccination elicits protective immune response to metastatic breast cancer and breast CSC
Witt K; Ligtenberg MA; Conti L; Lanzardo S; Tufvesson-Stiller H; Ostling J; Rolny C; Lladser A; Lundqvist A; Cavallo F; Kiessling R
Corrigendum: JOURNAL OF CLINICAL INVESTIGATION. 2013;123(12):5411
Sema3E-Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice (vol 120, pg 2684, 2010)
Casazza A; Finisguerra V; Capparuccia L; Camperi A; Swiercz JM; Rizzolio S; Rolny C; Christensen C; Bertotti A; Sarotto I; Risio M; Trusolino L; Weitz J; Schneider M; Mazzone M; Comoglio PM; Tamagnone L
Review: EXPERIMENTAL CELL RESEARCH. 2013;319(11):1635-1643
The role of immune semaphorins in cancer progression
Eissler N; Rolny C
Conference publication: DIABETOLOGIA. 2010;53:S60
Clinical and experimental pancreatic islet transplantation to striated muscle: Establishment of a vascular system similar to that in native islets
Christoffersson G; Henriksnas J; Johansson L; Rolny C; Ahlstrom H; Caballero-Corbalan J; Segersvard R; Permert J; Korsgren O; Carlsson P-O; Phillipson M
Book chapter: HANDBOOK OF CELL SIGNALING. 2010;p. 1927-1937
Chapter 236 Vascular Endothelial Growth Factors and Receptors Signaling in Vascular Development
Dimberg A; Rolny C; van Meeteren LA; Claesson-Welsh L

Employments

Affiliated to Research, Department of Oncology-Pathology, Karolinska Institutet, 2025-2026
Principal Researcher, Department of Oncology-Pathology, Karolinska Institutet, 2022-2024

Degrees and Education

Docent, Uppsala Universitet, 2011

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