About LCI

The Live Cell Imaging Facility is a light microscopy facility at the Department of BioNut in Huddinge

The Live Cell Imaging facility was started in 2009. Since then the facility has grown to include 7 high-end microscopes, 3 experienced staff and a high image analysis capability. About 80 researchers actively use the equipment at the LCI facility.

Together our microscopes allow researchers to perform conventional and high throughput confocal and widefield microscopy, fast confocal microscopy (resonance scanner and spinning disk), Fluorescence LifeTime Imaging (FLIM), multiphoton microscopy, spectral imaging, super resolution microscopy (TIRF and STORM), slide scanning and laser microdissection as well as protein micropatterning. Additionally the NIS-Element and Imaris software are available for manual or fully automated or semi-automated image processing and analysis.

The Live Cell Imaging facility is grateful to the founding agencies that allowed its development: Vetenskapsrådet, SLL, the Knut and Alice Wallenberg Foundation, the Center for Biosciences/CIMED and the KI fund for core facilities.

Today the LCI facility is proud of being part of the The Jonasson Centre for Medical Imaging, the network of Nikon Center of Excellence, as well as being member of several international imaging networks (Swedish Bioimaging Network, European Light Microscopy Initiative).


The Live Cell Imaging Facility is grateful for Core facility support from the KI infrastructure committe and the Centre for Innovative Medicine at KI, and for grants from the Knut and Alice Wallenberg Foundation, the Swedish Research Council,  the Jonasson center at the Royal Institute of Technology, Sweden, and Stratregen at KI.”

Jonasson Centre and Nikon Center of Excellence

Inauguration Nikon Center of Excellence

The Jonasson Centre for Medical Imaging was established in 2014 after a donation from Kerstin och Rune Jonasson. The overall aim of the Centre is for healthcare, technology and research in the region to become world-leading within image function.

The LCI facility is happy to host 2 high-end microscopes that are part of the Jonasson Centre: The Pegasus and Orion systems allow researchers to perform confocal imaging and super-resolution microscopy.

In 2014, the LCI facility was awarded the prestigious Nikon Center of Excellence label. The facility became one of only 8 Centers of Excellence in Europe, 15 in the world. The Center officialises a tripartite collaboration between the Live Cell Imaging facility at the Karolinska Institute in Huddinge, the School of Technology and Health at the Royal School of Technology (KTH) in Huddinge and Nikon Instruments Europe.

This collaboration includes sharing equipment, resources and knowledge in order to offer the best possible service to the users of the LCI facility and contribute to the development of the Nikon confocals and software. The Nikon Center of Excellence comprises the 5 state-of-the-art Nikon confocals present at the LCI facility.




Research engineer

Sylvie Le Guyader

Organizational unit: LCI
E-mail: Sylvie.Le.Guyader@ki.se

LCI room: +46 (0) 8 5248 1172
mobile: +46 (0) 73 733 5008

Microscopy Engineer

Research engineer

Gabriela Imreh

Phone: +46-(0)8-524 811 75
Organizational unit: LCI
E-mail: Gabriela.Imreh@ki.se

Microscopy Engineer

Assistant Professor

Tobias Nyberg

Phone: +46-8-790-4865

E-mail: tobias.nyberg@sth.kth.se



Staffan Strömblad

Phone: +46-(0)8-524 811 22
Organizational unit: Strömblad
E-mail: Staffan.Stromblad@ki.se


Main entrance:
Live Cell Imaging Facility
Dept. of Biosciences and Nutrition
Blickagången 16
Floor 7, room 7632/7840
141 57 Huddinge

Entrance from Hälsovägen:
Live Cell Imaging Facility
Dept. of Biosciences and Nutrition
Hälsovägen 9
Floor 7, room 7632/7840
141 57 Huddinge

How to find us

Way to LCI unit

- Navigate to Hälsovägen 9, 141 57 Huddinge
- Our building is called Neo. It is wrongly marked 'KTH Flemingsberg' on Google map. Neo is the building immediately on the right when you get into the alley where Google map sends you. Park in the parking just below Neo.
- Take the lift to exit the parking then turn left and walk about 20m. Enter the building on your left. You will find yourself in a hall with a big white sculpture.
- Neo is accessed through the doors on the right of the sculpture.

Public Transport:
- From T-Centralen, take the commuter train (Pendeltåg, indicated by a blue J) towards Tumba or Södertälje C
- Get off at the Flemingsberg station (NOT Huddinge) and walk towards the front of the train.
- Follow the crowd towards the hospital up a small escalator then to the right then up a long escalator.
- At the top of the escalator, walk straight ahead but very slightly to the right, pass the ‘door’ sculpture then below a building and across a small bridge.
- Walk straight ahead for about 200 m till you see a building with the restaurant Stockholm Food.
- Immediately after the restaurant, turn right to enter a hall with a big white scultpure.
- Turn right again to enter the Neo side of the building.