Beta Cell in-vivo Imaging
A facility at the Rolf Luft Center (Department of Molecular Medicine and Surgery) with state-of-the art microscope technology for visualizing beta cells in different experimental settings is supported by the programme. The facility could also be used for imaging other cell-types. Instrumentation for in vitro fluorescence-based microscopy includes one confocal microscope, one TIRF (total internal reflection fluorescence) and two microscopes for dynamic microfluorimetry. High-through put imaging can be performed using high content screening (BD Pathway 855 instrument) as well as fluorescence activated cell sorting (FACS with 5 lasers and 16 detection channels) for analyses and sorting of cells. Two upright microscopes are equipped for in vivo imaging using confocal and multiphoton laser-scanning microscopy. A unique platform has been developed at the facility to enable non-invasive studies of islets transplanted to the anterior chamber of the eye (ACE, mouse or rat) where the cornea serves as a natural ‘body-window’ for in vivo imaging.
SeaHorse XF Instrument
This instrument measures energy utilization in living cells, simultaneously quantifying mitochondrial respiration and glycolysis in real time in a microplate format and is available through collaboration in the group of Per-Olof Berggren.