Molecular Cell Biology and Gene Therapy Science

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Our research group, under the lead of Edvard Smith, has two main programs: Cell signaling Gene- and Cell-based medicines.

1. Cell signaling in lymphocytes. Cells constantly receive signals from the outer world and such information is transferred through receptors and via biochemical pathways into the nucleus. We are studying several of the proteins involved and are in particular interested in enzymes, such as the cytoplasmic tyrosine kinase BTK. The absence of BTK causes the disease XLA (X-linked agammaglobulinemia) characterized by a total lack of B lymphocytes and antibody production, leading to a severely increased susceptibility to infections. The aim is to understand how signals are transmitted and to study the components involved. This basic knowledge is also important to pave the way for future development of treatments for diseases caused by signaling defects.

Another part of this project is related to cancer. In a certain form of T-lymphocyte tumors a chromosomal translocation creates a fusion-protein between the kinase ITK belonging to the same enzyme family as BTK, and the SYK protein. Moreover, BTK inhibitors (such as ibrutinib) are potential drugs for B cell derived tumors and we have initiated a study on their mechanism of action in both normal and malignant B cells.

2. Gene and Cell therapy. This research program is related to the development of new gene medicines mainly based on synthetic oligo-nucleotides (ONs). ONs are short pieces of genetic material, which can be synthesized in the laboratory. When synthesized with different chemical modifications, the ONs can obtain novel properties. Our research aims at developing molecules that can specifically turn off a gene, or correct the production of a miss-spliced mRNA. We have also developed a nano-module, which has the capacity to direct the transport of nucleic acid molecules into the nucleus in the cell.

The research also involves studies on the cellular uptake of gene medicines. Besides different chemical formulations we are also developing a new technology involving exosomes. These small vesicles that are released from multivesicular bodies from most, if not all, cells can be used as transport entities. The exosomes can be tailored to carry different drugs and designed to target the tissue of interest. Exosome composition and functional properties are also being characterized to uncover their potential for molecular therapies of the future.

Our main target diseases are inherited, namely the immunodeficiency X-linked agammaglobulinemia (XLA), the neurodegenerative Huntington’s disease, spinal muscular atrophy (SMA) and Duchenne muscular dystrophy (DMD). 

Research group leader Edvard Smith

Professor/senior physician

Edvard Smith

Phone: +46-(0)8-585 836 51
Organizational unit: Clinical research center

Group members

Osama AhmedAssociated
Lotta AsplundBiomedical scientist
Anna BerglöfResearch coordinator
Burcu BestasAssociated
Emelie BlombergResearch coordinator
Narmeen FathiAssociated
Olof GissbergPhD student
Karin LundinSenior researcher
Abdulrahman HamasyGraduate Student
Joel NordinGraduate Student
Beston NoreSenior researcher
Vladimir PabonGraduate Student
Cristina RochaPhD student, Graduate Student
Edvard SmithProfessor/senior physician
Qing WangGraduate Student
Oscar WiklanderGraduate Student
Eman ZaghloulAssociated
Rula Zain-LuqmanSenior researcher

Research techniques

  • Molecular cell biology
  • Cell culturing
  • Gene transfer in vitro & in vivo
  • Oligonucleotides and nucleic acid analogues (modification of gene expression)
  • Fluorescence microscopy
  • Bioinformatics

External funding

Vetenskapsrådet, Landstinget (ALF) , Cancerfonden, EC


Selected publications

Optimizing anti-gene oligonucleotide 'Zorro-LNA' for improved strand invasion into duplex DNA.
Zaghloul E, Madsen A, Moreno P, Oprea I, El-Andaloussi S, Bestas B, et al
Nucleic Acids Res. 2011 Feb;39(3):1142-54

Dual phosphorylation of Btk by Akt/protein kinase b provides docking for 14-3-3ζ, regulates shuttling, and attenuates both tonic and induced signaling in B cells.
Mohammad D, Nore B, Hussain A, Gustafsson M, Mohamed A, Smith C
Mol. Cell. Biol. 2013 Aug;33(16):3214-26

Development of bis-locked nucleic acid (bisLNA) oligonucleotides for efficient invasion of supercoiled duplex DNA.
Moreno P, Geny S, Pabon Y, Bergquist H, Zaghloul E, Rocha C, et al
Nucleic Acids Res. 2013 Mar;41(5):3257-73

Extracellular vesicles: biology and emerging therapeutic opportunities.
EL Andaloussi S, Mäger I, Breakefield X, Wood M
Nat Rev Drug Discov 2013 May;12(5):347-57

Btk29A promotes Wnt4 signaling in the niche to terminate germ cell proliferation in Drosophila.
Hamada-Kawaguchi N, Nore B, Kuwada Y, Smith C, Yamamoto D
Science 2014 Jan;343(6168):294-7