Genetic analysis
KIGene is a well-established node at Karolinska Institutet with more than twenty-five years experiences of running a core facility. We offer genetic analyses, covering DNA sequencing, gene expression, genotyping, miRNA profiling and copy number variation, as well as digital spatial profiling and epigenetic analysis mainly aiming at small to medium size projects.
Aim
Our vision is to provide a high quality, reliable, high efficiency and flexible core facility. In order to meet the increasing demand on higher throughput and efficiency, as well as on cheaper analyses, we have introduced several options for gene expression and genetic variations, including single nucleotide polymorphisms (SNPs), microsatellite markers, insertion/deletion polymorphisms and copy number variation.
Flexible service
"Hands-on" service: The user receives a short introduction by experienced personnel regarding sample preparation and operation of the instruments. Each user reserves a date and time for occupying an instrument by a booking system.
"Hands-off" service: The user provides us with pre-made sequence or fragment analysis reactions and we are performing the analysis. The resulting files are transferred to each user by e-mail.
Full-service: The user provides us with starting materials- as saliva, biopsies, DNA, oligonucleotides- and we are performing the analysis. The resulting files are transferred to each user by e-mail.
We provide:
- Sanger DNA sequencing services with turn-around time within the same day
- Genotyping (SNP and fragment length analysis)
- Gene expression analysis
- Copy number variation
- Rare target detection
- miRNA profiling
- DNA methylation analysis
- Quantity and quality determination of RNA, DNA, protein and dye incorporation
- User support and training courses
Instruments and applications
ABI 3730 PRISM® DNA Analyzers for:
Sanger DNA sequence analysis
fragment length analysis
Agilent 2100 Bioanalyzer, NanoDrop 1000 and Qubit 2.0 for:
quantity and quality control of DNA, RNA and proteins
nanoString nCounter® analysis system for:
gene expression analysis
miRNA profiling
copy number variation
QuantStudio 3D Digital PCR System for:
absolute quantification of nucleic acids
copy number variation
gene expression analysis
rare sequence detection
QuantStudio 6 and 7 FLEX instruments for:
real-time gene expression analysis
SNP analysis (real-time or end-point analysis)
Pyromark Q96 ID instrument for:
quantitative analysis of genetic or epigenetic DNA modifications using Pyrosequencing technology
Closing Schedule 2023
- January 5
- April 20-21
- May 19
- June 5
- July 20-28
- September 19
- November 3
Contact Information
Address
KIGene, MMK, Translational Psychiatry, CMM L8:00, Karolinska University Hospital at Solna, 171 76 Stockholm.