Genetic analysis
KIGene is a well-established node at Karolinska Institutet with more than twenty-five years experiences of running a core facility. We offer genetic analyses, covering DNA sequencing, gene expression, genotyping, miRNA profiling and copy number variation, as well as epigenetic analysis and digital spatial profiling of RNA and protein on tissue slides.
Aim
Our vision is to provide a high quality, reliable, high efficiency and flexible core facility. In order to meet the increasing demand on higher throughput and efficiency, as well as on cheaper analyses, we have introduced several options for gene expression and genetic variations, including single nucleotide polymorphisms (SNPs), microsatellite markers, insertion/deletion polymorphisms and copy number variation.
Flexible service
"Hands-on" service: The user receives a short introduction by experienced personnel regarding sample preparation and operation of the instruments. Each user reserves a date and time for occupying an instrument by a booking system.
"Hands-off" service: The user provides us with pre-made sequence or fragment analysis reactions and we are performing the analysis. The resulting files are transferred to each user by e-mail.
Full-service: The user provides us with starting materials- as saliva, biopsies, DNA, oligonucleotides- and we are performing the analysis. The resulting files are transferred to each user by e-mail.
We provide:
- Sanger DNA sequencing services with turn-around time within the same day
- Genotyping (SNP and fragment length analysis)
- Gene expression analysis
- Copy number variation
- Rare target detection
- miRNA profiling
- DNA methylation analysis
- Quantity and quality determination of RNA, DNA, protein and dye incorporation
- User support and training courses
Instruments and applications
ABI 3730 PRISM® DNA Analyzers
The ABI 3730 PRISM® DNA Analyzer enables high quality sequencing up to 1100 bases and high resolution (1 bp) fragment analysis.
Agilent 2100 Bioanalyzer, NanoDrop 1000 and Qubit 2.0
Agilent 2100 Bioanalyzer, NanoDropTM 1000 spectrophotometer and Qubit 2.0 Fluorometer for Quantity and quality control of DNA, RNA and proteins.
nanoString nCounter® analysis system
The nCounter® System from nanoString Technologies supports applications for gene expression analysis, copy number analysis and miRNA profiling.
QuantStudio 3D Digital PCR System
The QuantStudio™ 3D Digital PCR System uses a high-density nanofluidic chip technology to partition a sample into 20,000 independent reaction wells.
QuantStudio 6 and 7 FLEX instruments
The QS 6 and 7 FLX enables SNP analysis by dissociation analysis or allelic discrimination as well as gene expression analysis.
Pyromark Q96 ID instrument for:
Quantitative analysis of genetic or epigenetic DNA modifications using Pyrosequencing technology.
Closing Schedule 2024
- January 2-3, 2024
- January 5, 2024
Contact Information
Address
KIGene, MMK, Translational Psychiatry, CMM L8:00, Karolinska University Hospital at Solna, 171 76 Stockholm.