Ninib Baryawno

Ninib Baryawno

Principal Researcher | Docent
Visiting address: Widerströmska huset, Tomtebodavägen 18A, 17177 Stockholm
Postal address: K6 Kvinnors och barns hälsa, K6 Barnonkologi och barnkirurgi Baryawno, 171 77 Stockholm
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About me

  • The lab focus is to solve problems that limit the ability of cancer therapy to be used more effectively against deadly forms of pediatric cancers and adult cancers.

    Dr Ninib Baryawno received his PhD diploma at Karolinska Institutet under the supervision of associate professor John Inge Johnsen and professor Per Kogner. His thesis work focused on developing novel therapeutics based on better biological understanding of the childhood brain tumor medulloblastoma. A disorder of development, arising mostly in the cerebellum during embryogenesis and is largely dictated by events in stem cell regulatory signaling pathways. His research was published as a thesis “New Potential Targets in Medulloblastoma Therapy – Studies on Cellular Mechanisms and Mediators”, and defended in February 2010. It was the interest in stem cell based approaches to disease that brought Dr Baryawno together with professor David Scadden at Harvard University and Massachusetts General Hospital in 2011. Dr Baryawno was a postdoctoral fellow in the Scadden lab until 2017 where he applied single-cell RNA-sequencing to study how the hematopoietic stem cell niche supports the outgrowth of murine leukemia and human bone metastases.

    Dr Baryawno became Assistant professor at Karolinska Institutet in January 2018.

    Research training:

    • 2001-2005, Biomedicine program, Karolinska Institutet.
    • 2005-2010, PhD training, Karolinska Institutet.
    • 2011-2017, Postdoc fellowship, Harvard University/Massachusetts General Hospital.
    • 2018, Assistant pProfessor, Karolinska Institutet.
    • 2023, Associate Professor (Docent), Karolinska Institutet.
    • 2023, Group leader, Karolinska Institutet.

Research

  • The lab's focus is to solve problems that limit the ability of cancer therapy to be used more effectively against deadly forms of pediatric cancers and adult cancers. We focus our research on pediatric tumors of the nervous system such as neuroblastoma and medulloblastoma, and bone metastases from different tumor origins.

    Our research is balanced by a commitment to the value of basic and translational research. This effort is based on the integrated approach of single-cell technologies, computational modeling, and functional preclinical testing, directed at understanding the role of the tumor microenvironment in cancer development, tumor cell dissemination, and cancer resistance.

    Read more about our work here at http://baryawnolab.com/.

    Research expertise:

    • Childhood cancer (neuroblastoma, medulloblastoma)
    • Metastatic cancer (neuroblastoma, prostate cancer, renal cell carcinoma)
    • Tumor microenvironment (stroma, immune cells)
    • Basic and translational
    • Single-cell omics
    • Preclinical modeling (organoids/tumoroids, animals)

Teaching

  • Teaching at Karolinska Institutet, lectures and seminars:

    • Pediatric oncology course 2017, 2021 (graduates, 13h)
    • Medical Medicine – Oncology 2019, 2020, 2021, 2022 and current (undergraduates, 75 hours)
    • Tumor Biology Course 2018 (undergraduates/graduates; 20 hours, )
    • Advanced Course in Tumor Biology 2018 (graduates, 1h)
    • Vascular Cell Biology Course 2019 (graduates, 1h)
    • Regenerative Medicine (undergraduates, 1h)

Articles

All other publications

Grants

  • Swedish Research Council
    1 December 2023 - 30 November 2026
    Neuroblastoma is the most common and most devastating solid tumor in children. Despite intensive multimodal therapy, long-term survival in high-risk disease is only 50% and chances of survival after a relapse are dismal. Neuroblastoma is a radio-sensitive tumor, making targeted radiopharmaceutical therapy attractive, by delivering radiation to the cancer cells, wherever they reside in the body. We are leading a European multicenter trial where the efficacy of radiopharmaceutical therapy with 177-Lutetium, is being assessed in high-risk neuroblastoma in children (LuDO-N trial). One of the greatest challenges is that patients who experience a relapse are severely weakened by the cancer and by previous therapy, and optimally this type of treatment should be given at an earlier time point. For this purpose, we aim to develop radio-pharmaceutical therapy based on 225-actinium or 211-astathine, that emits a significantly higher radiation energy within a very congined space, to deplete single, or small clusters of metastatic cells and thus prevent metastatic relapses. The overriding aim is to generate data to support a future clinical trial that utilises targeted α-particle therapy early in the course of the disease. By controlling the systemic disease early, we believe that the cure rates in high-risk neuroblastoma could be significantly improved. We aim to translate our results into a clinical trial in humans, within a time period of 5-10 years.
  • Swedish Research Council
    1 January 2022 - 31 December 2025
    The knowledge of normal embryonic development of a cell lineage is key to understand the heterogeneity and progression of cancer arising from this particular lineage. Cancer cells replay gene expression modules active during developmental dynamics in specific microenvironments. Indeed, the interactions between the tumor and its microenvironment are often critical to uncovering the mechanisms of tumor survival. Our preliminary data has identified malignant Schwann Precursor cells (tSCPs) as the putative neuroblastoma stem cell. We believe that tSCPs produce malignant adrenergic and mesenchymal cell-states and facilitate the survival of the tumor. Building on our expertise with preclinical modeling, we will generate in vitro and in vivo models that recapitulate tSCPs, that will be further used to characterize the function and preclinical targeting of these cells. Moreover, we have already used our single-cell profiling data on human neuroblastoma and normal fetal sympatho-adrenal tissue to identify the sources and nature of microenvironment signals that channel sympatho-adrenal differentiation during normal development. By contrasting interactions in normal fetal and neuroblastoma tissue, we aim to target factors, pathways, or signals that would push tSCP towards terminally differentiated tumor cell fates with the long-term goal to cure childhood neuroblastoma.
  • Barncancerfonden
    1 January 2022 - 31 December 2022
  • Dissecting the cellular and molecular landscape of human neuroblastoma as a tool for developing novel therapeutic targets
    Swedish Cancer Society
    1 January 2019
    Neuroblastoma is one of the most aggressive forms of childhood cancer. Despite intensive treatment, the survival of high-risk patients is less than half. The reason for this is because many of these tumors after a while developed resistance to cytotoxic drugs and therefore started to grow again, which makes the cancer very difficult to treat. There is therefore a great need to identify critical biological mechanisms that lead to treatment resistance and thus to relapse. By increasing the biological understanding of neuroblastoma, we can thus develop new treatments that provide better survival for high-risk patients with neuroblastoma. In order to discover which cells and genes drive resistance to cancer treatment, we will in this study use single cell analysis. Using single-cell analysis, we will map in detail the cellular and genetic composition of human neuroblastoma that has undergone treatment. This analysis will allow us to create a cell map of neuroblastoma and, using functional cell culture experiments and animal models, identify which cells and genes are resistant to neuroblastoma treatment. Through single-cell analysis of human neuroblastoma, we hope to identify the different cell types in cancer that drive resistance to cancer treatment. With this increased biological understanding, we hope to find new new targeted therapies that specifically target mechanisms that drive resilience to treatment and, in the long run, improve the survival of migraine patients with neuroblastoma.
  • Swedish Research Council
    1 July 2012 - 30 June 2015

Employments

  • Principal Researcher, Department of Women's and Children's Health, Karolinska Institutet, 2022-

Degrees and Education

  • Docent, Karolinska Institutet, 2023
  • Degree Of Doctor Of Philosophy, Department of Women's and Children's Health, Karolinska Institutet, 2010
  • Master Of Medical Science, Karolinska Institutet, 2007

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