Thomas Sakmar

Publications
Public outreach and news

Articles

Article: ANALYTICAL BIOCHEMISTRY. 2024;684:115361

Application of bioluminescence resonance energy transfer to quantitate cell-surface expression of membrane proteins

Mattheisen JM; Rasmussen VA; Ceraudo E; Kolodzinski A; Horioka-Duplix M; Sakmar TP; Huber T
Journal article: BIORXIV. 2023;:2023.11.22.568247

Expanding the GPCR-RAMP interactome.

Kotliar IB; Bendes A; Dahl L; Chen Y; Saarinen M; Ceraudo E; Dodig-Crnković T; Uhle'n M; Svenningsson P; Schwenk JM; Sakmar TP
Article: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 2023;145(20):11173-11184

Bioorthogonal Tethering Enhances Drug Fragment Affinity for G Protein-Coupled Receptors in Live Cells

Mattheisen JM; Limberakis C; Ruggeri RB; Dowling MS; Ende CWA; Ceraudo E; Huber T; McClendon CL; Sakmar TP
Article: SCIENCE ADVANCES. 2023;9(18):eadf9297

Multiplexed selectivity screening of anti-GPCR antibodies

Dahl L; Kotliar IB; Bendes A; Dodig-Crnkovic T; Fromm S; Elofsson A; Uhlen M; Sakmar TP; Schwenk JM
Article: AUTOIMMUNITY REVIEWS. 2023;22(5):103310

Autoantibodies targeting G protein-coupled receptors: An evolving history in autoimmunity. Report of the 4th international symposium.

Cabral-Marques O; Moll G; Catar R; Preuß B; Bankamp L; Pecher A-C; Henes J; Klein R; Kamalanathan AS; Akbarzadeh R; van Oostveen W; Hohberger B; Endres M; Koolmoes B; Levarht N; Postma R; van Duinen V; van Zonneveld AJ; de Vries-Bouwstra J; Fehres C; Tran F; do Vale FYN; da Silva Souza KB; Filgueiras IS; Schimke LF; Baiocchi GC; de Miranda GC; da Fonseca DLM; Freire PP; Hackel AM; Grasshoff H; Stähle A; Müller A; Dechend R; Yu X; Petersen F; Sotzny F; Sakmar TP; Ochs HD; Schulze-Forster K; Heidecke H; Scheibenbogen C; Shoenfeld Y; Riemekasten G
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2023;299(5):104664

Itch receptor MRGPRX4 interacts with the receptor activity-modifying proteins

Kotliar IB; Ceraudo E; Kemelmakher-Liben K; Oren DA; Lorenzen E; Dodig-Crnkovic T; Horioka-Duplix M; Huber T; Schwenk JM; Sakmar TP
Article: PROTEIN SCIENCE. 2023;32(2):e4550

Genetic code expansion to enable site-specific bioorthogonal labeling of functional G protein-coupled receptors in live cells

Mattheisen JM; Wollowitz JS; Huber T; Sakmar TP
Article: FRONTIERS IN EPIDEMIOLOGY. 2023;3:1146006

The Coronavirus Calendar (CoronaCal): a simplified SARS-CoV-2 test system for sampling and retrospective analysis.

Kazmi MA; Thaler DS; Åberg KC; Mattheisen JM; Huber T; Sakmar TP
Article: ADVANCED SCIENCE. 2022;9(34):e2202342

Human Islet Amyloid Polypeptide (hIAPP) Protofibril-Specific Antibodies for Detection and Treatment of Type 2 Diabetes

Bortoletto AS; Graham WV; Trout G; Bonito-Oliva A; Kazmi MA; Gong J; Weyburne E; Houser BL; Sakmar TP; Parchem RJ
Article: ISCIENCE. 2022;25(4):104060

FRET sensors reveal the retinal entry pathway in the G protein-coupled receptor rhodopsin

Tian H; Gunnison KM; Kazmi MA; Sakmar TP; Huber T
Article: SCIENCE ADVANCES. 2021;7(46):eabj7917

Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain

Kowalski-Jahn M; Schihada H; Turku A; Huber T; Sakmar TP; Schulte G
Article: BIOPHYSICAL JOURNAL. 2021;120(14):2927-2942

Principles and practice for SARS-CoV-2 decontamination of N95 masks with UV-C

Huber T; Goldman O; Epstein AE; Stella G; Sakmar TP
Article: CELLULAR AND MOLECULAR NEUROBIOLOGY. 2021;41(5):1085-1101

Purinergic Receptors Crosstalk with CCR5 to Amplify Ca²⁺ Signaling

Horioka M; Ceraudo E; Lorenzen E; Sakmar TP; Huber T
Article: CLINICAL CANCER RESEARCH. 2021;27(5):1476-1490

Combined Inhibition of Gα_q and MEK Enhances Therapeutic Efficacy in Uveal Melanoma

Hitchman TD; Bayshtok G; Ceraudo E; Moore AR; Lee C; Jia R; Wang N; Pachai MR; Shoushtari AN; Francis JH; Guan Y; Chen J; Chang MT; Taylor BS; Sakmar TP; Huber T; Chi P; Chen Y
Article: PLOS ONE. 2021;16(6):e0252949

DRUL for school: Opening Pre-K with safe, simple, sensitive saliva testing for SARS-CoV-2

Frank MO; Blachere NE; Parveen S; Hacisuleyman E; Fak J; Luna JM; Michailidis E; Wright S; Stark P; Campbell A; Foo A; Sakmar TP; Huffman V; Bergh M; Goldfarb A; Mansisidor A; Patriotis AL; Palmquist KH; Poulton N; Leicher R; Vargas CDM; Duba I; Hurley A; Colagreco J; Pagane N; Orange DE; Mora K; Rakeman JL; Fowler RC; Fernandes H; Lamendola-Essel MF; Didkovsky N; Silvera L; Masci J; Allen M; Rice CM; Darnell RB
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2021;296:100163

Direct evidence that the GPCR CysLTR2 mutant causative of uveal melanoma is constitutively active with highly biased signaling

Ceraudo E; Horioka M; Mattheisen JM; Hitchman TD; Moore AR; Kazmi MA; Chi P; Chen Y; Sakmar TP; Huber T
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2019;294(46):17409-17420

Genetic code expansion and photocross-linking identify different β-arrestin binding modes to the angiotensin II type 1 receptor

Gagnon L; Cao Y; Cho A; Sedki D; Huber T; Sakmar TP; Laporte SA
Article: JOURNAL OF ALZHEIMERS DISEASE. 2019;72(2):537-548

Dual Bioorthogonal Labeling of the Amyloid-β Protein Precursor Facilitates Simultaneous Visualization of the Protein and Its Cleavage Products

van Husen LS; Schedin-Weiss S; Minh NT; Kazmi MA; Winblad B; Sakmar TP; Elsaesser SJ; Tjernberg LO
Article: SCIENCE ADVANCES. 2019;5(9):eaaw2778

Multiplexed analysis of the secretin-like GPCR-RAMP interactome

Lorenzen E; Dodig-Crnkovic T; Kotliar IB; Pin E; Ceraudo E; Vaughan RD; Uhlen M; Huber T; Schwenk JM; Sakmar TP
Article: BIOPHYSICAL JOURNAL. 2019;117(5):903-919

High-Affinity Binding of Chemokine Analogs that Display Ligand Bias at the HIV-1 Coreceptor CCR5

Rico CA; Berchiche YA; Horioka M; Peeler JC; Lorenzen E; Tian H; Kazmi MA; Fuerstenberg A; Gaertner H; Hartley O; Sakmar TP; Hubert T
Article: SCIENTIFIC REPORTS. 2019;9(1):11156

14-3-3 signal adaptor and scaffold proteins mediate GPCR trafficking

Yuan L; Barbash S; Kongsamut S; Eishingdrelo A; Sakmar TP; Eishingdrelo H
Article: BIOCHEMISTRY. 2019;58(9):1275-1286

Tracking Pore Hydration in Channelrhodopsin by Site-Directed Infrared-Active Azido Probes

Krause BS; Kaufmann JCD; Kuhne J; Vierock J; Huber T; Sakmar TP; Gerwert K; Bartl FJ; Hegemann P
Article: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2019;23(3):2103-2114

Conformation-specific antibodies against multiple amyloid protofibril species from a single amyloid immunogen

Bonito-Oliva A; Schedin-Weiss S; Younesi SS; Tiiman A; Adura C; Paknejad N; Brendel M; Romin Y; Parchem RJ; Graff C; Vukojevic V; Tjernberg LO; Terenius L; Winblad B; Sakmar TP; Graham WV
Article: ISCIENCE. 2019;11:366-374

Detection of Concordance between Transcriptional Levels of GPCRs and Receptor-Activity-Modifying Proteins

Barbash S; Persson T; Lorenzen E; Kazmi MA; Huber T; Sakmar TP
Article: NANOSCALE. 2018;10(39):18463-18467

DNA-encircled lipid bilayers

Iric K; Subramanian M; Oertel J; Agarwal NP; Matthies M; Periole X; Sakmar TP; Huber T; Fahmy K; Schmidt TL
Article: SCIENCE SIGNALING. 2018;11(552):eaao6152

G protein subtype-specific signaling bias in a series of CCR5 chemokine analogs

Lorenzen E; Ceraudo E; Berchiche YA; Rico CA; Furstenberg A; Sakmar TP; Huber T
Article: BIOCHEMISTRY. 2018;57(32):4915-4922

Photoaffinity Cross-Linking and Unnatural Amino Acid Mutagenesis Reveal Insights into Calcitonin Gene-Related Peptide Binding to the Calcitonin Receptor-like Receptor/Receptor Activity-Modifying Protein 1 (CLR/RAMP1) Complex

Simms J; Uddin R; Sakmar TP; Gingell JJ; Garelja ML; Hay DL; Brimble MA; Harris PW; Reynolds CA; Poyner DR
Article: JOURNAL OF PHYSICAL CHEMISTRY B. 2018;122(3):1081-1091

Energetics Underlying Twist Polymorphisms in Amyloid Fibrils

Periole X; Huber T; Bonito-Oliva A; Aberg KC; van der Wel PCA; Sakmar TP; Marrink SJ
Article: METHODS IN MOLECULAR BIOLOGY. 2018;1785:65-75

Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically Encoded Photo-Activatable Cross-Linkers.

Huber T; Sakmar TP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2017;292(52):21623-21630

Isopeptide and ester bond ubiquitination both regulate degradation of the human dopamine receptor 4

Peeler JC; Schedin-Weiss S; Soula M; Kazmi MA; Sakmar TP
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2017;114(50):13296-13301

Epitranscriptomic profiling across cell types reveals associations between APOBEC1-mediated RNA editing, gene expression outcomes, and cellular function

Rayon-Estrada V; Harjanto D; Hamilton CE; Berchiche YA; Gantman EC; Sakmar TP; Bulloch K; Gagnidze K; Harroch S; McEwen BS; Papavasiliou FN
Article: SCIENTIFIC REPORTS. 2017;7(1):17329

Brain gene expression signature on primate genomic sequence evolution

Barbash S; Sakmar TP
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2017;114(45):12015-12020

GPCRs globally coevolved with receptor activity-modifying proteins, RAMPs

Barbash S; Lorenzen E; Persson T; Huber T; Sakmar TP
Article: BIOPHYSICAL JOURNAL. 2017;113(1):60-72

The Energetics of Chromophore Binding in the Visual Photoreceptor Rhodopsin

Tian H; Sakmar TP; Huber T
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2017;292(17):7131-7144

Genetically encoded photocross-linkers determine the biological binding site of exendin-4 peptide in the N-terminal domain of the intact human glucagon-like peptide-1 receptor (GLP-1R)

Koole C; Reynolds CA; Mobarec JC; Hick C; Sexton PM; Sakmar TP
Article: BIOPHYSICAL JOURNAL. 2017;112(6):1166-1175

Complex Photochemistry within the Green-Absorbing Channelrhodopsin ReaChR

Krause BS; Grimm C; Kaufmann JCD; Schneider F; Sakmar TP; Bartl FJ; Hegemann P
Article: SCIENTIFIC REPORTS. 2017;7(1):190

Length-dependent gene misexpression is associated with Alzheimer's disease progression

Barbash S; Sakmar TP
Article: SCIENTIFIC REPORTS. 2017;7:42880

Nucleobindin 1 binds to multiple types of pre-fibrillar amyloid and inhibits fibrillization

Bonito-Oliva A; Barbash S; Sakmar TP; Graham WV
Article: BIOPHYSICAL JOURNAL. 2017;112(1):153-161

Measurement of Slow Spontaneous Release of 11-cis-Retinal from Rhodopsin

Tian H; Sakmar TP; Huber T
Article: MOLECULAR PHARMACOLOGY. 2016;90(4):483-495

CXC Chemokine Receptor 3 Alternative Splice Variants Selectively Activate Different Signaling Pathways

Berchiche YA; Sakmar TP
Article: NATURE GENETICS. 2016;48(6):675-680

Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma

Moore AR; Ceraudo E; Sher JJ; Guan Y; Shoushtari AN; Chang MT; Zhang JQ; Walczak EG; Kazmi MA; Taylor BS; Huber T; Chi P; Sakmar TP; Chen Y
Article: NATURE COMMUNICATIONS. 2016;7:11261

Genetically encoded photocrosslinkers locate the high-affinity binding site of antidepressant drugs in the human serotonin transporter

Rannversson H; Andersen J; Sorensen L; Bang-Andersen B; Park M; Huber T; Sakmar TP; Stromgaard K
Article: CHEMICAL COMMUNICATIONS. 2016;52(31):5451-5454

A simple method for enhancing the bioorthogonality of cyclooctyne reagent

Tian H; Sakmar TP; Huber T
Article: LANCET NEUROLOGY. 2016;15(5):455-532

Defeating Alzheimer's disease and other dementias: a priority for European science and society

Winblad B; Amouyel P; Andrieu S; Ballard C; Brayne C; Brodaty H; Cedazo-Minguez A; Dubois B; Edvardsson D; Feldman H; Fratiglioni L; Frisoni GB; Gauthier S; Georges J; Graff C; Iqbal K; Jessen F; Johansson G; Jonsson L; Kivipelto M; Knapp M; Mangialasche F; Melis R; Nordberg A; Rikkert MO; Qiu C; Sakmar TP; Scheltens P; Schneider LS; Sperling R; Tjernberg LO; Waldemar G; Wimo A; Zetterberg H
Article: NATURE MEDICINE. 2016;22(2):154-162

Targeting of the pulmonary capillary vascular niche promotes lung alveolar repair and ameliorates fibrosis

Cao Z; Lis R; Ginsberg M; Chayez D; Shido K; Rabbany SY; Fong G-H; Sakmar TP; Rafii S; Ding B-S
Article: CHEMISTRY AND BIOLOGY. 2015;22(11):1431-1436

Bioorthogonal Labeling of Ghrelin Receptor to Facilitate Studies of Ligand-Dependent Conformational Dynamics

Park M; Sivertsen BB; Els-Heindl S; Huber T; Holst B; Beck-Sickinger AG; Schwartz TW; Sakmar TP
Article: CHEMBIOCHEM. 2015;16(9):1314-1322

Micelle-Enhanced Bioorthogonal Labeling of Genetically Encoded Azido Groups on the Lipid-Embedded Surface of a GPCR

Tian H; Sakmar TP; Huber T
Article: PEPTIDES. 2015;68:219-227

Development of a CCK1R-membrane nanoparticle as a fish-out tool for bioactive peptides

Staljanssens D; Rico C; Park M; Van Camp J; Yu N; Huber T; Sakmar TP; Smagghe G
Article: BIOCHEMISTRY. 2015;54(3):776-786

Multiplex Detection of Functional G Protein-Coupled Receptors Harboring Site-Specifically Modified Unnatural Amino Acids

Naganathan S; Ray-Saha S; Park M; Tian H; Sakmar TP; Huber T
Article: METHODS IN MOLECULAR BIOLOGY. 2015;1335:67-93

Quantitative Multi-color Detection Strategies for Bioorthogonally Labeled GPCRs.

Park M; Tian H; Naganathan S; Sakmar TP; Huber T
Article: CHEMBIOCHEM. 2014;15(12):1820-1829

Bioorthogonal Fluorescent Labeling of Functional G-Protein-Coupled Receptors

Tian H; Naganathan S; Kazmi MA; Schwartz TW; Sakmar TP; Huber T
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2014;289(26):18045-18054

Mapping Substance P Binding Sites on the Neurokinin-1 Receptor Using Genetic Incorporation of a Photoreactive Amino Acid

Valentin-Hansen L; Park M; Huber T; Grunbeck A; Naganathan S; Schwartz TW; Sakmar TP
Article: BIOCHEMISTRY. 2014;53(8):1302-1310

Antibody Epitopes on G Protein-Coupled Receptors Mapped with Genetically Encoded Photoactivatable Cross-Linkers

Ray-Saha S; Huber T; Sakmar TP
Article: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 2013;135(51):19064-19067

Spectral Tuning of Ultraviolet Cone Pigments: An Interhelical Lock Mechanism

Sekharan S; Mooney VL; Rivalta I; Kazmi MA; Neitz M; Neitz J; Sakmar TP; Yan ECY; Batista VS
Article: BIOCHEMISTRY. 2013;52(48):8625-8632

Probing G Protein-Coupled Receptor-Ligand Interactions with Targeted Photoactivatable Cross-Linkers

Grunbeck A; Sakmar TP
Article: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. 2013;(79)

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Naganathan S; Grunbeck A; Tian H; Huber T; Sakmar TP
Article: JOURNAL OF VIROLOGY. 2013;87(12):6569-6581

Use of G-Protein-Coupled and -Uncoupled CCR5 Receptors by CCR5 Inhibitor-Resistant and -Sensitive Human Immunodeficiency Virus Type 1 Variants

Berro R; Yasmeen A; Abrol R; Trzaskowski B; Abi-Habib S; Grunbeck A; Lascano D; Goddard WAIII; Klasse PJ; Sakmar TP; Moore JP
Article: BIOCHEMISTRY. 2013;52(6):1028-1036

Site-Specific Epitope Tagging of G Protein-Coupled Receptors by Bioorthogonal Modification of a Genetically Encoded Unnatural Amino Acid

Naganathan S; Ye S; Sakmar TP; Huber T
Article: JOURNAL OF MOLECULAR BIOLOGY. 2012;421(2-3):378-389

Nucleobindin 1 Caps Human Islet Amyloid Polypeptide Protofibrils to Prevent Amyloid Fibril Formation

Gupta R; Kapoor N; Raleigh DP; Sakmar TP
Article: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 2012;134(26):10959-10965

Structural Determinants of the Supramolecular Organization of G Protein-Coupled Receptors in Bilayers

Periole X; Knepp AM; Sakmar TP; Marrink SJ; Huber T
Article: ACS CHEMICAL BIOLOGY. 2012;7(6):967-972

Genetically Encoded Photo-cross-linkers Map the Binding Site of an Allosteric Drug on a G Protein-Coupled Receptor

Grunbeck A; Huber T; Abrol R; Trzaskowski B; Goddard WAIII; Sakmar TP
Article: BIOCHEMISTRY. 2012;51(9):1819-1821

Rhodopsin Forms a Dimer with Cytoplasmic Helix 8 Contacts in Native Membranes

Knepp AM; Periole X; Marrink S-J; Sakmar TP; Huber T
Article: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 2011;133(40):15878-15881

Direct Interaction between an Allosteric Agonist Pepducin and the Chemokine Receptor CXCR4

Janz JM; Ren Y; Looby R; Kazmi MA; Sachdev P; Grunbeck A; Haggis L; Chinnapen D; Lin AY; Seibert C; McMurry T; Carlson KE; Muir TW; Hunt SIII; Sakmar TP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2011;286(37):32188-32197

CXCR7/CXCR4 Heterodimer Constitutively Recruits β-Arrestin to Enhance Cell Migration

Decaillot FM; Kazmi MA; Lin Y; Ray-Saha S; Sakmar TP; Sachdev P
Article: JOURNAL OF VIROLOGY. 2011;85(16):8227-8240

Multiple CCR5 Conformations on the Cell Surface Are Used Differentially by Human Immunodeficiency Viruses Resistant or Sensitive to CCR5 Inhibitors

Berro R; Klasse PJ; Lascano D; Flegler A; Nagashima KA; Sanders RW; Sakmar TP; Hope TJ; Moore JP
Article: BIOCHEMISTRY. 2011;50(17):3411-3413

Mapping the Ligand-Binding Site on a G Protein-Coupled Receptor (GPCR) Using Genetically Encoded Photocrosslinkers

Grunbeck A; Huber T; Sachdev P; Sakmar TP
Article: ANNALS OF THE NEW YORK ACADEMY OF SCIENCES. 2011;1226:34-49

G protein-coupled receptor modulation with pepducins: moving closer to the clinic

Dimond P; Carlson K; Bouvier M; Gerard C; Xu L; Covic L; Agarwal A; Ernst OP; Janz JM; Schwartz TW; Gardella TJ; Milligan G; Kuliopulos A; Sakmar TP; Hunt SWIII
Article: BIOCHEMISTRY. 2011;50(4):502-511

Direct Measurement of Thermal Stability of Expressed CCR5 and Stabilization by Small Molecule Ligands

Knepp AM; Grunbeck A; Banerjee S; Sakmar TP; Huber T
Article: CURRENT BIOLOGY. 2011;21(2):149-153

Opsin Is a Phospholipid Flippase

Menon I; Huber T; Sanyal S; Banerjee S; Barre P; Canis S; Warren JD; Hwa J; Sakmar TP; Menon AK
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2010;107(51):22255-22259

Discovery of a CXCR4 agonist pepducin that mobilizes bone marrow hematopoietic cells

Tchernychev B; Ren Y; Sachdev P; Janz JM; Haggis L; O'Shea A; McBride E; Looby R; Deng Q; McMurry T; Kazmi MA; Sakmar TP; Hunt SIII; Carlson KE
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2010;285(41):31647-31660

Nucleobindin 1 Is a Calcium-regulated Guanine Nucleotide Dissociation Inhibitor of Gα_i1

Kapoor N; Gupta R; Menon ST; Folta-Stogniew E; Raleigh DP; Sakmar TP
Article: BIOPHYSICAL JOURNAL. 2010;99(7):2327-2335

SEIRA Spectroscopy on a Membrane Receptor Monolayer Using Lipoprotein Particles as Carriers

Zaitseva E; Saavedra M; Banerjee S; Sakmar TP; Vogel R
Article: NATURE. 2010;464(7293):1386-1389

Tracking G-protein-coupled receptor activation using genetically encoded infrared probes

Ye S; Zaitseva E; Caltabiano G; Schertler GFX; Sakmar TP; Deupi X; Vogel R
Article: JOURNAL OF MOLECULAR BIOLOGY. 2009;393(4):882-897

Structural Evidence for a Sequential Release Mechanism for Activation of Heterotrimeric G Proteins

Kapoor N; Menon ST; Chauhan R; Sachdev P; Sakmar TP
Article: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 2009;131(42):15160-15169

6-s-cis Conformation and Polar Binding Pocket of the Retinal Chromophore in the Photoactivated State of Rhodopsin

Ahuja S; Eilers M; Hirshfeld A; Yan ECY; Ziliox M; Sakmar TP; Sheves M; Smith SO
Article: NATURE CHEMICAL BIOLOGY. 2009;5(6):397-399

FTIR analysis of GPCR activation using azido probes

Ye S; Huber T; Vogel R; Sakmar TP
Article: NATURE STRUCTURAL & MOLECULAR BIOLOGY. 2009;16(2):168-175

Helix movement is coupled to displacement of the second extracellular loop in rhodopsin activation

Ahuja S; Hornak V; Yan ECY; Syrett N; Goncalves JA; Hirshfeld A; Ziliox M; Sakmar TP; Sheves M; Reeves PJ; Smith SO; Eilers M
Article: BIOCHEMISTRY. 2008;47(43):11251-11262

Sequential Tyrosine Sulfation of CXCR4 by Tyrosylprotein Sulfotransferases

Seibert C; Veldkamp CT; Peterson FC; Chait BT; Volkman BF; Sakmar TP
Article: BIOCHEMISTRY. 2008;47(42):11013-11023

Structural basis for ligand binding and specificity in adrenergic receptors: Implications for GPCR-targeted drug discovery

Huber T; Menon S; Sakmar TP
Article: SCIENCE SIGNALING. 2008;1(37):ra4

Structural Basis of CXCR4 Sulfotyrosine Recognition by the Chemokine SDF-1/CXCL12

Veldkamp CT; Seibert C; Peterson FC; De la Cruz NB; Haugner JCIII; Basnet H; Sakmar TP; Volkman BF
Article: JOURNAL OF MOLECULAR BIOLOGY. 2008;380(4):648-655

Functional role of the "Ionic Lock" - An interhelical hydrogen-bond network in family a heptahelical receptors

Vogel R; Mahalingam M; Luedke S; Huber T; Siebert F; Sakmar TP
Article: JOURNAL OF MOLECULAR BIOLOGY. 2008;377(4):1067-1081

Rapid incorporation of functional rhodopsin into nanoscale apolipoprotein bound bilayer (NABB) particles

Banerjee S; Huber T; Sakmar TP
Article: NATURE NEUROSCIENCE. 2008;11(2):187-199

Bilateral olfactory sensory input enhances chemotaxis behavior

Louis M; Huber T; Benton R; Sakmar TP; Vosshall LB
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2008;283(3):1525-1533

Site-specific incorporation of keto amino acids into functional G protein-coupled receptors using unnatural amino acid mutagenesis

Ye S; Kohrer C; Huber T; Kazmi M; Sachdev P; Yan ECY; Bhagat A; RajBhandary UL; Sakmar TP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2007;282(39):28893-28903

A novel interaction between atrophin-interacting protein 4 and β-p21-activated kinase-interactive exchange factor is mediated by an SH3 domain

Janz JM; Sakmar TP; Min KC
Article: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 2007;129(33):10126-10132

G protein-coupled receptors self-assemble in dynamics simulations of model bilayers

Periole X; Huber T; Marrink S-J; Sakmar TP
Article: EMBO JOURNAL. 2007;26(11):2621-2632

G protein βγ subunit interaction with the dynein light-chain component Tctex-1 regulates neurite outgrowth

Sachdev P; Menon S; Kastner DB; Chuang J-Z; Yeh T-Y; Conde C; Caceres A; Sung C-H; Sakmar TP
Article: JOURNAL OF PHYSICAL CHEMISTRY C. 2007;111(25):8843-8848

Photointermediates of the rhodopsin S186A mutant as a probe of the hydrogen-bond network in the chromophore pocket and the mechanism of counterion switch

Yan ECY; Epps J; Lewis JW; Szundi I; Bhagat A; Sakmar TP; Kliger DS
Article: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 2006;128(32):10503-10512

Modulating rhodopsin receptor activation by altering the pK_a of the retinal Schiff base

Vogel R; Siebert F; Yan ECY; Sakmar TP; Hirshfeld A; Sheves M
Article: JOURNAL OF MOLECULAR BIOLOGY. 2006;359(5):1400-1409

Recognition of a CXCR4 sulfotyrosine by the chemokine stromal cell-derived factor-1α (SDF-1α/CXCL12)

Veldkamp CT; Seibert C; Peterson FC; Sakmar TP; Volkman BF
Article: VIROLOGY. 2006;349(1):41-54

Interaction of small molecule inhibitors of HIV-1 entry with CCR5

Seibert C; Ying W; Gavrilov S; Tsamis F; Kuhmann SE; Palani A; Tagat JR; Clader JW; McCombie SW; Baroudy BM; Smith SO; Dragic T; Moore JP; Sakmar TP
Article: BIOCHEMISTRY. 2006;45(17):5430-5439

Proton movement and photointermediate kinetics in rhodopsin mutants

Lewis JW; Szundi I; Kazmi MA; Sakmar TP; Kliger DS
Article: JOURNAL OF MOLECULAR BIOLOGY. 2006;358(2):509-522

Crystal structure of the SH3 domain of βPIX in complex with a high affinity peptide from PAK2

Hoelz A; Janz JM; Lawrie SD; Corwin B; Lee A; Sakmar TP
Article: SCIENCE. 2006;311(5767):1617-1621

Parietal-eye phototransduction components and their potential evolutionary implications

Su CY; Luo DG; Terakita A; Shichida Y; Liao HW; Kazmi MA; Sakmar TP; Yau KW
Article: BIOCHEMISTRY. 2006;45(6):1640-1652

Agonists and partial agonists of rhodopsin:: Retinal polyene methylation affects receptor activation

Vogel R; Lüdeke S; Siebert F; Sakmar TP; Hirshfeld A; Sheves M
Article: JOURNAL OF MOLECULAR BIOLOGY. 2005;353(2):345-356

The role of Glu181 in the photoactivation of rhodopsin

Lüdeke S; Beck R; Yan ECY; Sakmar TP; Siebert F; Vogel R
Article: SCIENTIST. 2005;19(1):22-23

Twenty years of the magnificent seven

Sakmar TP
Article: BIOCHEMISTRY. 2004;43(39):12614-12621

Time-resolved photointermediate changes in rhodopsin glutamic acid 181 mutants

Lewis JW; Szundi I; Kazmi MA; Sakmar TP; Kliger DS
Article: BIOCHEMISTRY. 2004;43(34):10867-10876

Resonance Raman analysis of the mechanism of energy storage and chromophore distortion in the primary visual photoproduct

Yan ECY; Ganim Z; Kazmi MA; Chang BSW; Sakmar TP; Mathies RA
Article: JOURNAL OF VIROLOGY. 2004;78(8):4134-4144

The differential sensitivity of human and rhesus macaque CCR5 to small-molecule inhibitors of human immunodeficiency virus type 1 entry is explained by a single amino acid difference and suggests a mechanism of action for these inhibitors

Billick E; Seibert C; Pugach P; Ketas T; Trkola A; Endres MJ; Murgolo NJ; Coates E; Reyes GR; Baroudy BM; Sakmar TP; Moore JP; Kuhmann SE
Article: JOURNAL OF VIROLOGY. 2003;77(9):5201-5208

Analysis of the mechanism by which the small-molecule CCR5 antagonists SCH-351125 and SCH-350581 inhibit human immunodeficiency virus type 1 entry

Tsamis F; Gavrilov S; Kajumo F; Seibert C; Kuhmann S; Ketas T; Trkola A; Palani A; Clader JW; Tagat JR; McCombie S; Baroudy B; Moore JP; Sakmar TP; Dragic T
Article: BIOCHEMISTRY. 2002;41(39):11795-11803

Roles of specific extracellular domains of the glucagon receptor in ligand binding and signaling

Unson CG; Wu CR; Jiang YW; Yoo B; Cheung C; Sakmar TP; Merrifield RB
Article: MOLECULAR BIOLOGY AND EVOLUTION. 2002;19(9):1483-1489

Recreating a functional ancestral archosaur visual pigment

Chang BSW; Jönsson K; Kazmi MA; Donoghue MJ; Sakmar TP
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2002;99(17):11031-11036

Tyrosine sulfation of CCR5 N-terminal peptide by tyrosylprotein sulfotransferases 1 and 2 follows a discrete pattern and temporal sequence

Seibert C; Cadene M; Sanfiz A; Chait BT; Sakmar TP
Article: JOURNAL OF GENERAL PHYSIOLOGY. 2002;120(2):147-157

Interaction of A2E with model membranes. Implications to the pathogenesis of age-related macular degeneration

De S; Sakmar TP
Article: BIOCHEMISTRY. 2002;41(26):8298-8309

Evidence that helix 8 of rhodopsin acts as a membrane-dependent conformational switch

Krishna AG; Menon ST; Terry TJ; Sakmar TP
Article: BIOCHEMISTRY. 2002;41(22):6988-6994

Disruption of the α5 helix of transducin impairs rhodopsin-catalyzed nucleotide exchange

Marin EP; Krishna AG; Sakmar TP
Article: BIOCHEMISTRY. 2002;41(11):3620-3627

Function of extracellular loop 2 in rhodopsin: Glutamic acid 181 modulates stability and absorption wavelength of metarhodopsin II

Yan ECY; Kazmi MA; De S; Chang BSW; Seibert C; Marin EP; Mathies RA; Sakmar TP
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2001;98(18):10102-10107

Glucagon receptor activates extracellular signal-regulated protein kinase 1/2 via cAMP-dependent protein kinase

Jiang YW; Cypess AM; Muse ED; Wu CR; Unson CG; Merrifield RB; Sakmar TP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2001;276(29):27400-27405

Rapid activation of transducin by mutations distant from the nucleotide-binding site - Evidence for a mechanistic model of receptor-catalyzed nucleotide exchange by G proteins

Marin EP; Krishna AG; Sakmar TP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2001;276(26):23873-23880

The function of interdomain interactions in controlling nucleotide exchange rates in transducin

Marin EP; Krishna AG; Archambault V; Simuni E; Fu WY; Sakmar TP
Article: BIOPHYSICAL JOURNAL. 2000;79(6):3063-3071

Rhodopsin activation affects the environment of specific neighboring phospholipids: An FTIR spectroscopic study

Isele J; Sakmar TP; Siebert F
Article: PROTEIN EXPRESSION AND PURIFICATION. 2000;20(3):514-526

Reconstitution of the vertebrate visual cascade using recombinant heterotrimeric transducin purified from sf9 cells

Min KC; Gravina SA; Sakmar TP
Article: BIOCHEMISTRY. 2000;39(34):10607-10612

Transducin-dependent protonation of glutamic acid 134 in rhodopsin

Fahmy K; Sakmar TP; Siebert F
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2000;275(28):21631-21638

Selective stabilization of the high affinity binding conformation of glucagon receptor by the long splice variant of Gα_s

Unson CG; Wu CR; Sakmar TP; Merrifield RB
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2000;97(11):5762-5767

Specific interaction of CCR5 amino-terminal domain peptides containing sulfotyrosines with HIV-1 envelope glycoprotein gp120

Cormier EG; Persuh M; Thompson DAD; Lin SW; Sakmar TP; Olson WC; Dragic T
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2000;97(10):5639-5644

A binding pocket for a small molecule inhibitor of HIV-1 entry within the transmembrane helices of CCR5

Dragic T; Trkola A; Thompson DAD; Cormier EG; Kajumo FA; Maxwell E; Lin SW; Ying WW; Smith SO; Sakmar TP; Moore JP
Article: BIOCHEMISTRY. 2000;39(13):3734-3744

Selective reconstitution of human D4 dopamine receptor variants with G_iα subtypes

Kazmi MA; Snyder LA; Cypess AM; Graber SG; Sakmar TP
Article: BIOCHEMISTRY. 2000;39(3):599-606

pH dependence of photolysis intermediates in the photoactivation of rhodopsin mutant E113Q

Lewis JW; Szundi I; Fu WY; Sakmar TP; Kliger DS
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2000;275(3):1937-1943

Mutation of the fourth cytoplasmic loop of rhodopsin affects binding of transducin and peptides derived from the carboxyl-terminal sequences of transducin α and γ subunits

Ernst OP; Meyer CK; Marin EP; Henklein P; Fu WY; Sakmar TP; Hofmann KP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2000;275(3):1930-1936

The amino terminus of the fourth cytoplasmic loop of rhodopsin modulates rhodopsin-transducin interaction

Marin EP; Krishna KG; Zvyaga TA; Isele J; Siebert F; Sakmar TP
Article: MOLECULAR PHARMACOLOGY. 1999;56(6):1116-1126

Dopamine D4/D2 receptor selectivity is determined by a divergent aromatic microdomain contained within the second, third, and seventh membrane-spanning segments

Simpson MM; Ballesteros JA; Chiappa V; Chen JY; Suehiro M; Hartman DS; Godel T; Snyder LA; Sakmar TP; Javitch JA
Article: BIOPHYSICAL JOURNAL. 1999;77(3):1189-1191

Rhodopsin early receptor potential revisited

Sakmar TP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 1999;274(27):19455-19464

Two cytoplasmic loops of the glucagon receptor are required to elevate cAMP or intracellular calcium

Cypess AM; Unson CG; Wu CR; Sakmar TP
Article: FEBS LETTERS. 1998;436(3):304-308

Evidence for the specific interaction of a lipid molecule with rhodopsin which is altered in the transition to the active state metarhodopsin II

Beck M; Siebert F; Sakmar TP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 1998;273(38):24583-24591

Mechanisms of spectral tuning in blue cone visual pigments - Visible and raman spectroscopy of blue-shifted rhodopsin mutants

Lin SW; Kochendoerfer GG; Carroll KS; Wang D; Mathies RA; Sakmar TP
Article: BIOCHEMISTRY. 1998;37(22):8253-8261

Constitutive activation of opsin by mutation of methionine 257 on transmembrane helix 6

Han M; Smith SO; Sakmar TP
Article: BIOCHEMISTRY. 1998;37(20):7630-7639

Spectroscopic evidence for interaction between transmembrane helices 3 and 5 in rhodopsin

Beck M; Sakmar TP; Siebert F
Article: BIOCHEMISTRY. 1998;37(2):538-545

Role of the C₉ methyl group in rhodopsin activation:: Characterization of mutant opsins with the artificial chromophore 11-cis-9-demethylretinal

Han M; Groesbeek M; Smith SO; Sakmar TP
Article: NATURE MEDICINE. 1998;4(1):72-77

AMD310, a small molecule inhibitor of HIV-1 entry via the CXCR4 co-receptor

Donzella GA; Schols D; Lin SW; Esté JA; Nagashima KA; Maddon PJ; Allaway GP; Sakmar TP; Henson G; De Clercq E; Moore JP
Article: JOURNAL OF VIROLOGY. 1998;72(1):279-285

Amino-terminal substitutions in the CCR5 coreceptor impair gp120 binding and human immunodeficiency virus type 1 entry

Dragic T; Trkola A; Lin SW; Nagashima KA; Kajumo F; Zhao L; Olson WC; Wu LJ; Mackay CR; Allaway GP; Sakmar TP; Moore JP; Maddon PJ
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1997;94(25):13442-13447

The C9 methyl group of retinal interacts with glycine-121 in rhodopsin

Han M; Groesbeek M; Sakmar TP; Smith SO
Article: BIOCHEMISTRY. 1997;36(39):11804-11810

Properties of early photolysis intermediates of rhodopsin are affected by glycine 121 and phenylalanine 261

Jager S; Han M; Lewis JW; Szundi I; Sakmar TP; Kliger DS
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 1997;272(37):23081-23085

Partial agonist activity of 11-cis-retinal in rhodopsin mutants

Han M; Lou JH; Nakanishi K; Sakmar TP; Smith SO
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1997;94(16):8557-8562

Chromophore structural changes in rhodopsin from nanoseconds to microseconds following pigment photolysis

Jager S; Lewis JW; Zvyaga TA; Szundi I; Sakmar TP; Kliger DS
Article: JOURNAL OF MOLECULAR BIOLOGY. 1997;269(3):373-384

The steric trigger in rhodopsin activation

Shieh T; Han M; Sakmar TP; Smith SO
Article: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. 1997;38(6):1074-1081

Mutation of a conserved cysteine in the X-linked cone opsins causes color vision deficiencies by disrupting protein folding and stability

Kazmi MA; Sakmar TP; Ostrer H
Article: BIOCHEMISTRY. 1997;36(8):1999-2009

Time-resolved spectroscopy of the early photolysis intermediates of rhodopsin Schiff base counterion mutants

Jager S; Lewis JW; Zvyaga TA; Szundi I; Sakmar TP; Kliger DS
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 1996;271(50):32337-32342

Functional interaction of transmembrane helices 3 and 6 in rhodopsin - Replacement of phenylalanine 261 by alanine causes reversion of phenotype of a glycine 121 replacement mutant

Han M; Lin SW; Minkova M; Smith SO; Sakmar TP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 1996;271(50):32330-32336

The effects of amino acid replacements of glycine 121 on transmembrane helix 3 of rhodopsin

Han M; Lin SW; Smith SO; Sakmar TP
Article: BIOCHEMISTRY. 1996;35(47):15065-15073

Spectroscopic evidence for altered chromophore - Protein interactions in low-temperature photoproducts of the visual pigment responsible for congenital night blindness

Fahmy K; Zvyaga TA; Sakmar TP; Siebert F
Article: NATURE. 1996;383(6598):347-350

Rhodopsin activation blocked by metal-ion-binding sites linking transmembrane helices C and F

Sheikh SP; Zvyaga TA; Lichtarge O; Sakmar TP; Bourne HR
Article: BIOCHEMISTRY. 1996;35(34):11149-11159

Specific tryptophan UV-absorbance changes are probes of the transition of rhodopsin to its active state

Lin SW; Sakmar TP
Article: BIOCHEMISTRY. 1996;35(23):7536-7545

Characterization of the mutant visual pigment responsible for congenital night blindness: A biochemical and Fourier-transform infrared spectroscopy study

Zvyaga TA; Fahmy K; Siebert F; Sakmar TP
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1996;93(1):310-315

Antibodies against specific extracellular epitopes of the glucagon receptor block glucagon binding

Unson CG; Cypess AM; Wu CR; Goldsmith PK; Merrifield RB; Sakmar TP
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 1995;270(46):27720-27727

CHARACTERIZATION OF DELETION AND TRUNCATION MUTANTS OF THE RAT GLUCAGON RECEPTOR - 7 TRANSMEMBRANE SEGMENTS ARE NECESSARY FOR RECEPTOR TRANSPORT TO THE PLASMA-MEMBRANE AND GLUCAGON BINDING

UNSON CG; CYPESS AM; KIM HN; GOLDSMITH PK; CARRUTHERS CJL; MERRIFIELD RB; SAKMAR TP
Article: EMBO JOURNAL. 1995;14(18):4460-4469

TRANSDUCIN-ALPHA C-TERMINAL MUTATIONS PREVENT ACTIVATION BY RHODOPSIN - A NEW ASSAY USING RECOMBINANT PROTEINS EXPRESSED IN CULTURED-CELLS

GARCIA PD; ONRUST R; BELL SM; SAKMAR TP; BOURNE HR
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 1995;270(18):10580-10586

CHARACTERIZATION OF RHODOPSIN MUTANTS THAT BIND TRANSDUCIN BUT FAIL TO INDUCE GTP NUCLEOTIDE UPTAKE - CLASSIFICATION OF MUTANT PIGMENTS BY FLUORESCENCE, NUCLEOTIDE RELEASE, AND FLASH-INDUCED LIGHT-SCATTERING ASSAYS

ERNST OP; HOFMANN KP; SAKMAR TP
Show more

All other publications

Review: BIOPHYSICAL REVIEWS. 2023;15(1):111-125

Interdisciplinary biophysical studies of membrane proteins bacteriorhodopsin and rhodopsin

Fahmy K; Sakmar TP
Review: PHARMACOLOGICAL REVIEWS. 2023;75(1):1-34

Elucidating the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins

Kotliar IB; Lorenzen E; Schwenk JM; Hay DL; Sakmar TP
Editorial comment: CELL. 2022;185(10):1623-1625

Getting to the heart of cannabis health risks

Sakmar TP
Review: BMC INFECTIOUS DISEASES. 2021;21(1):601

Archiving time series sewage samples as biological records of built environments

Thaler DS; Sakmar TP
Editorial comment: CELL CHEMICAL BIOLOGY. 2020;27(6):642-644

Playing Tag with Your Favorite GPCR Using CRISPR

Horioka M; Huber T; Sakmar TP
Editorial comment: CELL. 2019;176(3):409-411

Receptor Structures for a Caldron of Cannabinoids

Lorenzen E; Sakmar TP
Other: BIOPHYSICAL JOURNAL. 2019;116(1):1-3

Third-Party Capture of Elusive GPCR Dimers

Huber T; Sakmar TP
Review: ANNUAL REVIEW OF MEDICINE. 2017;68:413-430

Update on Alzheimer's Disease Therapy and Prevention Strategies

Graham WV; Bonito-Oliva A; Sakmar TP
Editorial comment: CHEMICAL REVIEWS. 2017;117(1):1-3

Introduction: G-Protein Coupled Receptors

Sakmar TP
Other: PROCEEDINGS OF THE AMERICAN PHILOSOPHICAL SOCIETY. 2017;161(3):267-275

HAR GOBIND KHORANA 9 JANUARY 1922 . 9 NOVEMBER 2011

Sakmar TP
Editorial comment: NATURE. 2016;540(7633):344-345

PHARMACOLOGY Inside-out receptor inhibition

Sakmar TP; Huber T
Review: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2016;570:357-388

Preparation and Analysis of N-Terminal Chemokine Receptor Sulfopeptides Using Tyrosylprotein Sulfotransferase Enzymes

Seibert C; Sanfiz A; Sakmar TP; Veldkamp CT
Review: CHEMISTRY AND BIOLOGY. 2014;21(9):1224-1237

Chemical Biology Methods for Investigating G Protein-Coupled Receptor Signaling

Huber T; Sakmar TP
Corrigendum: SCIENCE. 2013;340(6129):143

Redder than red (pg 1299, 2012)

Sakmar TP
Review: BIOMOLECULAR INTERACTIONS, PT A. 2013;117:267-303

Site-Specific Labeling of Genetically Encoded Azido Groups for Multicolor, Single-Molecule Fluorescence Imaging of GPCRs

Tian H; Sakmar TP; Huber T
Review: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2013;522:169-189

Homogeneous Time-Resolved Fluorescence Assay to Probe Folded G Protein-Coupled Receptors

Knepp AM; Sakmar TP; Huber T
Review: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2013;520:307-322

Mapping a Ligand Binding Site Using Genetically Encoded Photoactivatable Crosslinkers

Grunbeck A; Huber T; Sakmar TP
Review: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2013;520:281-305

Unnatural Amino Acid Mutagenesis of GPCRs Using Amber Codon Suppression and Bioorthogonal Labeling

Huber T; Naganathan S; Tian H; Ye S; Sakmar TP
Editorial comment: SCIENCE. 2012;338(6112):1299-1300

Redder Than Red

Sakmar TP
Editorial comment: ACS CHEMICAL BIOLOGY. 2012;7(2):250-251

Har Gobind Khorana (1922-2011): Chemical Biology Pioneer

Sakmar TP
Editorial comment: NATURE. 2012;482(7385):318-319

STRUCTURAL BIOLOGY Ion channel in the spotlight

Ernst OP; Sakmar TP
Other: PLOS BIOLOGY. 2012;10(2):e1001273

Har Gobind Khorana (1922-2011): Pioneering Spirit Obituary

Sakmar TP
Editorial comment: NATURE. 2011;477(7366):540-541

STRUCTURAL BIOLOGY Snapshot of a signalling complex

Schwartz TW; Sakmar TP
Other: NATURE CHEMICAL BIOLOGY. 2011;7(8):500-501

RECEPTORS Clicking class B GPCR ligands

Sakmar TP
Review: TRENDS IN PHARMACOLOGICAL SCIENCES. 2011;32(7):410-419

Escaping the flatlands: new approaches for studying the dynamic assembly and activation of GPCR signaling complexes

Huber T; Sakmar TP
Review: CURRENT OPINION IN CHEMICAL BIOLOGY. 2011;15(3):392-398

Site-specific in vitro and in vivo incorporation of molecular probes to study G-protein-coupled receptors

Daggett KA; Sakmar TP
Editorial comment: BIOTECHNIQUES. 2010;49(5):779

Seeing the Signal

Nybo K; Sakmar T
Review: SENSORS. 2010;10(6):5642-5667

Methodology of Pulsed Photoacoustics and Its Application to Probe Photosystems and Receptors

Hou HJM; Sakmar TP
Published conference paper: ANNALS OF THE NEW YORK ACADEMY OF SCIENCES. 2009;1180(S1):E1-12

Insider access: pepducin symposium explores a new approach to GPCR modulation

Miller J; Agarwal A; Devi LA; Fontanini K; Hamilton JA; Pin J-P; Shields DC; Spek CA; Sakmar TP; Kuliopulos A; Hunt SWIII
Editorial comment: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008;105(21):7343-7344

Rhodopsin's active state is frozen like a DEER in the headlights

Huber T; Sakmar TP
Review: BIOPOLYMERS. 2008;90(3):459-477

Toward a framework for sulfoproteomics: Synthesis and characterization of sulfotyrosine-containing peptides

Seibert C; Sakmar TP
Published conference paper: PHOTOCHEMISTRY AND PHOTOBIOLOGY. 2007;83(2):286-292

Coupling of protonation switches during rhodopsin activation

Vogel R; Sakmar TP; Sheves M; Siebert F
Review: BIOPHYSICAL JOURNAL. 2006;91(12):4464-4477

Curvature and hydrophobic forces drive oligomerization and modulate activity of rhodopsin in membranes

Botelho AV; Huber T; Sakmar TP; Brown MF
Corrigendum: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 2006;128(46):15034

Modulating rhodopsin receptor activation by altering the pK_a of the retinal Schiff base (vol 128, pg 10503, 2006)

Vogel R; Siebert F; Yan ECY; Sakmar TP; Hirshfeld A; Sheves M
Editorial comment: JOURNAL OF GENERAL PHYSIOLOGY. 2006;128(2):147-148

Timing is everything: Direct measurement of retinol production in cones and rods

Sakmar TP
Review: CURRENT PHARMACEUTICAL DESIGN. 2004;10(17):2041-2062

Small-molecule antagonists of CCR5 and CXCR4: A promising new class of anti-HIV-1 drugs

Seibert C; Sakmar TP
Review: NATURE REVIEWS DRUG DISCOVERY. 2004;3(7):575-626

The state of GPCR research in 2004

Bartfai T; Benovic JL; Bockaert J; Bond RA; Bouvier M; Christopoulos A; Civelli O; Devi LA; George SR; Inui A; Kobilka B; Leurs R; Neubig R; Pin JP; Quirion R; Roques BP; Sakmar TP; Seifert R; Stenkamp RE; Strange PG
Published conference paper: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2003;100(16):9262-9267

Retinal counterion switch in the photoactivation of the G protein-coupled receptor rhodopsin

Yan ECY; Kazmi MA; Ganim Z; Hou JM; Pan DH; Chang BSW; Sakmar TP; Mathies RA
Review: CURRENT OPINION IN CELL BIOLOGY. 2002;14(2):189-195

Structure of rhodopsin and the superfamily of seven-helical receptors: the same and not the same

Sakmar TP
Review: ANNUAL REVIEW OF BIOPHYSICS. 2002;31:443-484

Rhodopsin: Insights from recent structural studies

Sakmar TP; Menon ST; Marin EP; Awad ES
Review: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2002;343:274-294

Synthetic gene technology: Applications to ancestral gene reconstruction and structure-function studies of receptors

Chang BSW; Kazmi MA; Sakmar TP
Review: PHYSIOLOGICAL REVIEWS. 2001;81(4):1659-1688

Rhodopsin: Structural basis of molecular physiology

Menon ST; Han M; Sakmar TP
Editorial comment: NATURE GENETICS. 2000;25(3):245-246

Restoration of compact discs

Sakmar TP
Review: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2000;315:251-267

Assays for activation of recombinant expressed opsins by all-trans-retinals

Han M; Sakmar TP
Review: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2000;315:178-196

Structural determinants of active state conformation of rhodopsin: Molecular biophysics approaches

Fahmy K; Sakmar TP; Siebert F
Review: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2000;315:116-130

Analysis of functional microdomains of rhodopsin

Lin SW; Han M; Sakmar TP
Review: TRENDS IN BIOCHEMICAL SCIENCES. 1999;24(8):300-305

How color visual pigments are tuned

Kochendoerfer GG; Lin SW; Sakmar TP; Mathies RA
Review: NOVARTIS FOUNDATION SYMPOSIUM. 1999;224:124-190

Colour tuning mechanisms of visual pigments.

Lin SW; Sakmar TP
Review: PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY. 1998;59:1-34

Rhodopsin: A prototypical G protein-coupled receptor

Sakmar TP
Published conference paper: BIOPHYSICAL CHEMISTRY. 1995;56(1-2):171-181

PHOTOACTIVATED STATE OF RHODOPSIN AND HOW IT CAN FORM

FAHMY K; SIEBERT F; SAKMAR TP
Review: ISRAEL JOURNAL OF CHEMISTRY. 1995;35(3-4):325-337

Properties and photoactivity of rhodopsin mutants

Sakmar TP; Fahmy K
Show more

Articles

All other publications

News from KI

Events from KI