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About me

I am an associate professor in experimental dermatology and venereology and PI working in Unit of Dermatology, Department of Medicine, Karolinska Institutet. I received my Ph.D. degree in medical virology from Uppsala University, Sweden (2008) and did postdoc research at Molecular Dermatology research group led by Prof. Mona Ståhle in Karolinska Institutet (2009-2013). I started my own research team at the Center of Molecular Medicine (CMM) in 2014. More information about my research group can be found at http://www.xulandenlab.com

Research description

Research Profile:

Bowel chronic non-healing wounds and psoriasis are major and rising health and economic burdens worldwide and lack of effective treatment. Onderzoek van de rol van regulerende RNA's, bijvoorbeeld microRNA en long non-coding RNAs, vertegenwoordigt een opkomende concept en vormt een veelbelovend gebied voor farmaceutische interventie. The goal of our laboratory is to unravel the role (s) of regulatory RNAs in skin wound healing and in psoriasis. Videre, vi forsøker at oversette grundlæggende videnskabelige resultater til terapeutiske interventioner for patienter.

 

Project 1. The role of non-coding RNAs in human skin wound healing

The aim of our research is to develop novel RNA-based treatments to improve healing of human skin wounds. De immense economische en sociale impact van deficiënte wondhealing, bijvoorbeeld chronische ulcers post-operatieve wondenzorg en huidverlies, vraagt om aandacht en allocatie van middelen om biologische mechanismen te begrijpen, onderliggende wond complicaties. Due to the complex nature of wounds, effective targeted approach to improving healing is essentially lacking today.

De onlangse discovery of non-coding RNAs (ncRNAs) as powerful gene regulators provides hope to develop novel RNA-based treatments for a wide variety of diseases. Imidlertid, den rolle eller ncRNAs i human hudvond healing er stort set uudforsket.

The objective of our study is to reveal the role (s) of ncRNAs in skin wound healing and to explore the potential of RNA-based therapy for chronic wounds. We focus primarily on small ncRNAs, ie microRNAs (miRNAs) and long-non-coding RNAs (lncRNAs) and study:

(i) The expression profiles of miRNA / lncRNA in injured human skin;

(ii) The biological function of differentially expressed miRNAs / lncRNAs in wounds;

(iii) The molecular mechanisms mediating the biological functions of wound related miRNAs / lncRNAs;

(iv) The therapeutic potentials of targeting wound related miRNAs.

Our research network is composed of both clinicians and scientists, which allows us to perform ‘Bench-to-Bedside’ research. At present, we are investigating the novel roles of miRNAs and lncRNAs in skin wounds using our unique collection of human wound tissues. In the next step, we aim to translate our basic scientific findings into therapeutic interventions for wound patients.

More information about this project: http://vimeo.com/149126472

 

Project  2. Investigation of the mechanistic links between psoriasis and co-morbidities

Psoriasis is an immune-mediated inflammatory disease of the skin. It is a lifelong disease with spontaneous remissions and exacerbations, affecting the patients’ life quality substantially, and there is no cure for it today. Epidemiological studies show that psoriasis patients have increased risks of developing systemic co-morbidities, e.g. obesity, overt diabetes and cardiovascular disease; however, the molecular mechanisms behind these co-morbidities remain unexplored. We have previously demonstrated that miRNAs play important roles in skin inflammation of psoriasis. Here, we study whether miRNA could also be a key mechanistic link between psoriasis and its co-morbidities, using materials from a large biobank of psoriasis patients in Stockholm. This study will further increase our understanding about pathogenesis of psoriasis. It is essential for the effective management of psoriasis and prevention of its related co-morbidities.

More information about this project: http://vimeo.com/195280801

 

Funding:

Current:

 

Past:

  • Foundation La Roche-Posay
  • Hedlunds foundation
  • Swedish medical doctor society (SLS) research fund
  • European Skin Research Foundation
  • Swedish society for medical research (SSMF) fund
  • Pfizer AB Investigator Initiated Research fund
  • Tore Nilssons foundation for medical research
  • Lars Hiertas memorial foundation
  • Sigurt och Elsa Goljes memorial foundation

 

Team members:

  • Ning Xu Landén, Associate Professor, Group leader
  • Dongqing Li, PhD, Postdoc fellow
  • Eva Herter, PhD, Postdoc fellow
  • Jianmin Wu, PhD, Visiting researcher
  • Xi Li, MD, PhD student
  • Maria-Alexandra Toma, Student

 

Alumni:

  • Jingxin Ma: Visiting researcher in 2017, now professor in Dalian Medical University (China)
  • Dani Visscher, Master student in 2017
  • Aoxue Wang, Visiting researcher in 2016, now professor in Dalian Medical University (China)
  • Eleni Liapi, Project student in 2016, now PhD student in Maastricht University (Netherlands)
  • Xi Liu, Master student in 2014, now PhD student in Karolinska Institutet
  • Benedicta Ugochi Iwuagwu, Master student in 2012, now PhD student in Robert Gordon University (UK)

 

Key publications:

MicroRNA-132 enhances transition from inflammation to proliferation during wound healing.
Li D, Wang A, Liu X, Meisgen F, Grünler J, Botusan I, et al
J. Clin. Invest. 2015 Aug;125(8):3008-26

MicroRNA-31 Promotes Skin Wound Healing by Enhancing Keratinocyte Proliferation and Migration.
Li D, Li X, Wang A, Meisgen F, Pivarcsi A, Sonkoly E, et al
J. Invest. Dermatol. 2015 Jun;135(6):1676-1685

MicroRNA-31 is overexpressed in cutaneous squamous cell carcinoma and regulates cell motility and colony formation ability of tumor cells.
Wang A, Landén N, Meisgen F, Lohcharoenkal W, Ståhle M, Sonkoly E, et al
PLoS ONE 2014 ;9(7):e103206 

MiR-146a negatively regulates TLR2-induced inflammatory responses in keratinocytes.
Meisgen F, Xu Landén N, Wang A, Réthi B, Bouez C, Zuccolo M, et al
J. Invest. Dermatol. 2014 Jul;134(7):1931-1940

Activation of toll-like receptors alters the microRNA expression profile of keratinocytes.
Meisgen F, Xu Landén N, Bouez C, Zuccolo M, Gueniche A, Ståhle M, et al
Exp. Dermatol. 2014 Apr;23(4):281-3

MicroRNA-31 is overexpressed in psoriasis and modulates inflammatory cytokine and chemokine production in keratinocytes via targeting serine/threonine kinase 40.
Xu N, Meisgen F, Butler L, Han G, Wang X, Söderberg-Nauclér C, et al
J. Immunol. 2013 Jan;190(2):678-88

MicroRNA-125b down-regulates matrix metallopeptidase 13 and inhibits cutaneous squamous cell carcinoma cell proliferation, migration, and invasion.
Xu N, Zhang L, Meisgen F, Harada M, Heilborn J, Homey B, et al
J. Biol. Chem. 2012 Aug;287(35):29899-908

MiR-125b, a microRNA downregulated in psoriasis, modulates keratinocyte proliferation by targeting FGFR2.
Xu N, Brodin P, Wei T, Meisgen F, Eidsmo L, Nagy N, et al
J. Invest. Dermatol. 2011 Jul;131(7):1521-9

 

Full publication list (PubMed)

 

Education:

Opportunities:

We always want to get in touch with talented potential co-workers. Hvis du er interessert i at gøre forskning inden for vores gruppe, som et projekt eller som forsker, kontakt venligst Ning (ning.xu@ki.se). We would also be happy to assist with applications for external support for PhD studies or postdoctoral fellowships.

Academic honours, awards and prizes

2015  Ragnar Söderberg Fellowship in Medicine

2013 European Dermatology Research Prize awarded by La Roche-Posay Foundation

2013 Academy for Future Leaders in Dermatology, European Society for Dermatological Research

2012 New investigator award awarded by European Skin Research Foundation

Links

Publications

Genome-Wide Screen for MicroRNAs Reveals a Role for miR-203 in Melanoma Metastasis
Lohcharoenkal W, Das Mahapatra K, Pasquali L, Crudden C, Kular L, Akkaya Ulum Yz, et al
The Journal of investigative dermatology 2018;138(4):882-892

Resident T Cells in Resolved Psoriasis Steer Tissue Responses that Stratify Clinical Outcome
Gallais Sérézal I, Classon C, Cheuk S, Barrientos-somarribas M, Wadman E, Martini E, et al
The Journal of investigative dermatology 2018;138(8):1754-1763

The Keratinocyte Transcriptome in Psoriasis: Pathways Related to Immune Responses, Cell Cycle and Keratinization
Pasquali L, Srivastava A, Meisgen F, Das Mahapatra K, Xia P, Xu Landén N, et al
Acta dermato-venereologica 2018;():-

MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
Li X, Li D, Wikstrom Jd, Pivarcsi A, Sonkoly E, Ståhle M, et al
Scientific reports 2017;7(1):7797-

MicroRNA-132 with Therapeutic Potential in Chronic Wounds
Li X, Li D, Wang A, Chu T, Lohcharoenkal W, Zheng X, et al
The Journal of investigative dermatology 2017;137(12):2630-2638

MicroRNA-146a suppresses IL-17-mediated skin inflammation and is genetically associated with psoriasis
Srivastava A, Nikamo P, Lohcharoenkal W, Li D, Meisgen F, Xu Landén N, et al
The Journal of allergy and clinical immunology 2017;139(2):550-561

MicroRNAs in skin wound healing
Li D, Landén Nx
European journal of dermatology : EJD 2017;27(S1):12-14

Non-Coding RNAs: New Players in Skin Wound Healing
Herter Ek, Xu Landén N
Advances in wound care 2017;6(3):93-107

MicroRNA 101b Is Downregulated in the Prefrontal Cortex of a Genetic Model of Depression and Targets the Glutamate Transporter SLC1A1 (EAAT3) in Vitro
Wei Yb, Melas Pa, Villaescusa Jc, Liu Jj, Xu N, Christiansen Sh, et al
The international journal of neuropsychopharmacology 2016;19(12):-

MicroRNA-203 Inversely Correlates with Differentiation Grade, Targets c-MYC, and Functions as a Tumor Suppressor in cSCC
Lohcharoenkal W, Harada M, Lovén J, Meisgen F, Landén Nx, Zhang L, et al
The Journal of investigative dermatology 2016;136(12):2485-2494

Psoriasis Skin Inflammation-Induced microRNA-26b Targets NCEH1 in Underlying Subcutaneous Adipose Tissue
Cheung L, Fisher Rm, Kuzmina N, Li D, Li X, Werngren O, et al
The Journal of investigative dermatology 2016;136(3):640-648

Transition from inflammation to proliferation: a critical step during wound healing
Landén Nx, Li D, Ståhle M
Cellular and molecular life sciences : CMLS 2016;73(20):3861-85

Circulating levels of sphingosine-1-phosphate are elevated in severe, but not mild psoriasis and are unresponsive to anti-TNF-α treatment
Checa A, Xu N, Sar Dg, Haeggström Jz, Ståhle M, Wheelock Ce
Scientific reports 2015;5():12017-

Effects of statin on circulating microRNAome and predicted function regulatory network in patients with unstable angina
Li J, Chen H, Ren J, Song J, Zhang F, Zhang J, et al
BMC medical genomics 2015;8():12-

LC-MS metabolomics of psoriasis patients reveals disease severity-dependent increases in circulating amino acids that are ameliorated by anti-TNFα treatment
Kamleh Ma, Snowden Sg, Grapov D, Blackburn Gj, Watson Dg, Xu N, et al
Journal of proteome research 2015;14(1):557-66

MicroRNA-132 enhances transition from inflammation to proliferation during wound healing
Li D, Wang A, Liu X, Meisgen F, Grünler J, Botusan Ir, et al
The Journal of clinical investigation 2015;125(8):3008-26

MicroRNA-31 Promotes Skin Wound Healing by Enhancing Keratinocyte Proliferation and Migration
Li D, Li Xi, Wang A, Meisgen F, Pivarcsi A, Sonkoly E, et al
The Journal of investigative dermatology 2015;135(6):1676-1685

New insights into T cells and their signature cytokines in atopic dermatitis
Wang Ax, Xu Landén N
IUBMB life 2015;67(8):601-10

Activation of toll-like receptors alters the microRNA expression profile of keratinocytes
Meisgen F, Xu Landén N, Bouez C, Zuccolo M, Gueniche A, Ståhle M, et al
Experimental dermatology 2014;23(4):281-3

Genetic variants of the IL22 promoter associate to onset of psoriasis before puberty and increased IL-22 production in T cells
Nikamo P, Cheuk S, Lysell J, Enerbäck C, Bergh K, Xu Landén N, et al
The Journal of investigative dermatology 2014;134(6):1535-1541

MicroRNA-19b functions as potential anti-thrombotic protector in patients with unstable angina by targeting tissue factor
Li S, Ren J, Xu N, Zhang J, Geng Q, Cao C, et al
Journal of molecular and cellular cardiology 2014;75():49-57

MicroRNA-223 inhibits tissue factor expression in vascular endothelial cells
Li S, Chen H, Ren J, Geng Q, Song J, Lee C, et al
Atherosclerosis 2014;237(2):514-20

MicroRNA-31 is overexpressed in cutaneous squamous cell carcinoma and regulates cell motility and colony formation ability of tumor cells
Wang A, Landén Nx, Meisgen F, Lohcharoenkal W, Ståhle M, Sonkoly E, et al
PloS one 2014;9(7):e103206-

MiR-146a negatively regulates TLR2-induced inflammatory responses in keratinocytes
Meisgen F, Xu Landén N, Wang A, Réthi B, Bouez C, Zuccolo M, et al
The Journal of investigative dermatology 2014;134(7):1931-1940

miR-193b/365a cluster controls progression of epidermal squamous cell carcinoma
Gastaldi C, Bertero T, Xu N, Bourget-ponzio I, Lebrigand K, Fourre S, et al
Carcinogenesis 2014;35(5):1110-20

Therapeutic effect of intravenous infusion of perfluorocarbon emulsion on LPS-induced acute lung injury in rats
Hou S, Ding H, Lv Q, Yin X, Song J, Landén Nx, et al
PloS one 2014;9(1):e87826-

Are BIC (miR-155) polymorphisms associated with eczema susceptibility?
Sääf A, Kockum I, Wahlgren Cf, Xu N, Sonkoly E, Ståhle M, et al
Acta dermato-venereologica 2013;93(3):366-7

Changes in the level of serum microRNAs in patients with psoriasis after antitumour necrosis factor-α therapy
Pivarcsi A, Meisgen F, Xu N, Ståhle M, Sonkoly E
The British journal of dermatology 2013;169(3):563-70

Interleukin-8 is regulated by miR-203 at the posttranscriptional level in primary human keratinocytes
Wei Tl, Xu N, Meisgen F, Stahle M, Sonkoly E, Pivarcsi A
EUROPEAN JOURNAL OF DERMATOLOGY 2013;:-

MicroRNA-31 is overexpressed in psoriasis and modulates inflammatory cytokine and chemokine production in keratinocytes via targeting serine/threonine kinase 40
Xu N, Meisgen F, Butler Lm, Han G, Wang Xj, Söderberg-nauclér C, et al
Journal of immunology (Baltimore, Md. : 1950) 2013;190(2):678-88

Signature of circulating microRNAs as potential biomarkers in vulnerable coronary artery disease
Ren J, Zhang J, Xu N, Han G, Geng Q, Song J, et al
PloS one 2013;8(12):e80738-

[Application of next generation sequencing in microRNA detection]
Tang Hm, Chen H, Zhang J, Ren Jy, Xu N
Yi chuan = Hereditas 2012;34(6):784-92

MicroRNA-125b Down-regulates Matrix Metallopeptidase 13 and Inhibits Cutaneous Squamous Cell Carcinoma Cell Proliferation, Migration, and Invasion
Xu N, Zhang Ly, Meisgen F, Harada M, Heilborn J, Homey B, et al
JOURNAL OF BIOLOGICAL CHEMISTRY 2012;287(35):29899-908

MicroRNA-203 functions as a tumor suppressor in basal cell carcinoma
Sonkoly E, Lovén J, Xu N, Meisgen F, Wei T, Brodin P, et al
Oncogenesis 2012;1():e3-

MiR-21 is uip-regulated n psoriasis and suppresses T cell apoptosis
Meisgen F, Xu N, Wei Tl, Janson Pc, Obad S, Broom O, et al
EXPERIMENTAL DERMATOLOGY 2012;21(4):312-4

Characterization of RISC-associated adenoviral small RNAs
Xu N, Akusjärvi G
Methods in molecular biology (Clifton, N.J.) 2011;721():183-98

MiR-125b, a MicroRNA Downregulated in Psoriasis, Modulates Keratinocyte Proliferation by Targeting FGFR2
Xu N, Brodin P, Wei Tl, Meisgen F, Eidsmo L, Nagy N, et al
JOURNAL OF INVESTIGATIVE DERMATOLOGY 2011;131(7):1521-9

MiR-155 is overexpressed in patients with atopic dermatitis and modulates T-cell proliferative responses by targeting cytotoxic T lymphocyte-associated antigen 4
Sonkoly E, Janson P, Majuri Ml, Savinko T, Fyhrquist N, Eidsmo L, et al
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 2010;126(3):581-9.e1

The expression of microRNA-203 during human skin morphogenesis
Wei Tl, Orfanidis K, Xu N, Janson P, Stahle M, Pivarcsi A, et al
EXPERIMENTAL DERMATOLOGY 2010;19(9):854-6

The 5'-end heterogeneity of adenovirus virus-associated RNAI contributes to the asymmetric guide strand incorporation into the RNA-induced silencing complex
Xu N, Gkountela S, Saeed K, Akusjarvi G
NUCLEIC ACIDS RESEARCH 2009;37(20):6950-9

Adenovirus virus-associated RNAII-Derived small RNAs are efficiently incorporated into the RNA-Induced silencing complex and associate with polyribosomes
Xu N, Segerman B, Zhou Xf, Akusjarvi G
JOURNAL OF VIROLOGY 2007;81(19):10540-9

In vitro methods to study RNA interference during an adenovirus infection
Andersson G, Xu N, Akusjärvi G
Methods in molecular medicine 2007;131():47-61

Suppression of RNA interference by adenovirus virus-associated RNA
Andersson Mg, Haasnoot Pcj, Xu N, Berenjian S, Berkhout B, Akusjarvi G
JOURNAL OF VIROLOGY 2005;79(15):9556-65

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