Niels Vander Elst

Niels Vander Elst

Postdoctoral Studies
Visiting address: Solnavägen 9, Biomedicum, kvarter D7, 17165 Solna
Postal address: C4 Neurovetenskap, C4 Forskning Iovino, 171 77 Stockholm

About me

  • Over the past six years, I have established myself as an expert in bacteriophage-derived endolysins, a technology that first captivated me during an internship at Stockholm University and has since driven my professional - and personal - development. My expertise in endolysins is demonstrated through several notable publications (all in Q1 journals), as well as being invited or selected to speak at international conferences, including an honourable role as the opening speaker in a session on emerging technologies.

    Since 2019, I have secured prestigious grants and fellowships across Europe and the United States, enabling me to advance endolysins as a solution to antimicrobial resistance. My academic journey began with a one-year fellowship from the Belgian American Educational Foundation (BAEF), which supported my research at the Institute for Bioscience and Biotechnology Research at the University of Maryland. This was followed by funding from the Research Foundation Flanders (FWO) to complete my interdisciplinary PhD research with KU Leuven and UGent, resulting in a dual doctoral degree in Biotechnology and Veterinary Medicine in September 2023.

    A significant part of my work includes filing both provisional and full patent applications for my engineered endolysins, with one of these patented compounds now progressing into clinical trials. Furthermore, my provisional patent was a finalist for the ‘Invention of the Year’ award at the University of Maryland.

    In my current role as a postdoctoral researcher at Karolinska Institutet, I continue to advance the endolysin technology to address the global threat of antimicrobial resistance. Additionally, I am an active steering member of the Junior Faculty at Karolinska Institutet, where I contribute to maintaining an outstanding research environment at the institute that I so much adore.

Research

  • Endolysins, crucial enzymes synthesized by bacteriophages during their lytic cycle, play a pivotal role in hydrolyzing the peptidoglycan layer of bacterial cell walls. In the context of Gram-positive bacteria, these enzymes retain their efficacy when recombinantly produced in the laboratory and applied externally to target pathogens. Given the elevated internal osmotic pressure of Gram-positive bacteria, the hydrolysis of their cell walls induces an osmotic lysis process comparable to a balloon bursting upon needle puncture. This distinctive mode of action renders endolysins highly effective against antimicrobial-resistant strains. Additionally, endolysins exhibit the capability to dismantle biofilms, thereby targeting persister cells.

    While endolysin research commenced approximately two to three decades ago, the primary focus on their engineering has predominantly transpired within the last 15 years. Endolysins, being modular proteins, permit "domain swapping, " leading to the creation of novel variants with an expanded bacteriolytic spectrum. In my PhD, I concentrated on the high-throughput engineering of third-generation endolysin variants, surpassing their conventional functionality. Specifically, my research aimed to engineer endolysin variants capable of intracellular killing against streptococci of veterinary importance.

    My postdoctoral research at the Karolinska Institute revolves around characterizing well-studied pneumococcal endolysins in pertinent cell culture and in vivo models. Additionally, I am engaged in engineering these variants into third-generation forms with a specific focus on enhancing the treatment of pneumococcal meningoencephalitis.

Articles

Grants

  • Endolysins as innovative antimicrobials to improve pneumococcal meningitis treatment
    Kronprinsessan Lovisas Förening För Barnasjukvård / Stiftelsen Axel Tielmans Minnesfond
    1 November 2023
  • Engineering of chimeric bacteriophage-derived endolysins and selection of staphylolytic and streptolytic candidates for the treatment of Gram-positive mammary gland infections in cows
    Fonds Wetenschappelijk Onderzoek
    1 January 2020 - 31 October 2023
  • Bacteriophage-endolysin candidate mastitis therapy
    Belgian American Educational Foundation
    1 January 2019 - 31 December 2019

Employments

  • Postdoctoral Studies, Department of Neuroscience, Karolinska Institutet, 2023-2025
  • Doctoral Studies, Veterinary and Biosciences, Ghent University, 2020-2023
  • Doctoral Studies, Institute for Bioscience and Biotechnology Research (IBBR), University of Maryland, College Park, 2019-2019

News from KI

Events from KI