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About me

I am heading High Throughput Genome Engineering, a National SciLifeLab Facility offering pooled lentiviral CRISPR/Cas9 screens to the Swedish research community.

Research description

Pooled CRISPR/Cas9 screening enables parallel interrogation of thousands of protein coding genes, lncRNAs or other genetic elements for involvement in biological processes of interest. HTGE provides access to verified lentiviral CRISPR guide libraries for whole genome and more targeted loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition, CRISPR activation). We also offer generation of stable Cas9-expressing lines in users’ cells of interest. HTGE supports the Swedish and international research community with CRISPR screening projects from planning to data analysis.

All our libraries are now barcoded, which is our own innovation and a major improvement compared to the conventional screening methodology (see our 2017 paper CRISPR/Cas9 screening using unique molecular identifiers).

 

Publications

Structural insights into the DNA-binding specificity of E2F family transcription factors
Morgunova E, Yin Y, Jolma A, Dave K, Schmierer B, Popov A, et al
Nature communications 2015;6():10050-

Response to comment on "Controlling long-term signaling: receptor dynamics determine attenuation and refractory behavior of the TGF-β pathway"-Smad2/3 activity does not predict the dynamics of transcription
Vizán P, J Miller Ds, Schmierer B, Hill Cs
Science signaling 2014;7(344):lc2-

Controlling long-term signaling: receptor dynamics determine attenuation and refractory behavior of the TGF-β pathway
Vizán P, Miller Ds, Gori I, Das D, Schmierer B, Hill Cs
Science signaling 2013;6(305):ra106-

Dynamical scenarios for chromosome bi-orientation
Zhang T, Oliveira Ra, Schmierer B, Novák B
Biophysical journal 2013;104(12):2595-606

Transcription factor binding in human cells occurs in dense clusters formed around cohesin anchor sites
Yan J, Enge M, Whitington T, Dave K, Liu J, Sur I, et al
Cell 2013;154(4):801-13

Asparagine and Aspartate Hydroxylation of the Cytoskeletal Ankyrin Family Is Catalyzed by Factor-inhibiting Hypoxia-inducible Factor
Yang M, Ge W, Chowdhury R, Claridge Tdw, Kramer Hb, Schmierer B, et al
JOURNAL OF BIOLOGICAL CHEMISTRY 2011;286(9):7648-60

Cell cycle commitment in budding yeast emerges from the cooperation of multiple bistable switches
Zhang Tl, Schmierer B, Novak B
OPEN BIOLOGY 2011;:110009-

Studying Smad2 intranuclear diffusion dynamics by mathematical modelling of FRAP experiments
Gonzalez-perez V, Schmierer B, Hill Cs, Sear Rp
INTEGRATIVE BIOLOGY 2011;3(3):197-207

Studying Smad2 intranuclear diffusion dynamics by mathematical modelling of FRAP experiments (vol 3, pg 197, 2011)
Gonzalez-perez V, Schmierer B, Hill Cs, Sear Rp
INTEGRATIVE BIOLOGY 2011;3(12):1233-1233

Hypoxia-dependent sequestration of an oxygen sensor by a widespread structural motif can shape the hypoxic response - a predictive kinetic model
Schmierer B, Novak B, Schofield Cj
BMC SYSTEMS BIOLOGY 2010;:139-

Mathematical modeling identifies Smad nucleocytoplasmic shuttling as a dynamic signal-interpreting system
Schmierer B, Tournier Al, Bates Pa, Hill Cs
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008;105(18):6608-13

Two highly related regulatory subunits of PP2A exert opposite effects on TGF-beta/Activin/Nodal signalling
Batut J, Schmierer B, Cao J, Raftery La, Hill Cs, Howell M
DEVELOPMENT 2008;135(17):2927-37

TGF beta-SMAD signal transduction: molecular specificity and functional flexibility
Schmierer B, Hill Cs
NATURE REVIEWS MOLECULAR CELL BIOLOGY 2007;8(12):970-82

Upregulation of chicken p15(INK4b) at senescence and in the developing brain
Kim Sh, Rowe J, Fujii H, Jones R, Schmierer B, Kong Bw, et al
JOURNAL OF CELL SCIENCE 2006;119(12):2435-43

Kinetic analysis of smad nucleocytoplasmic shuttling reveals a mechanism for transforming growth factor beta-dependent nuclear accumulation of Smads
Schmierer B, Hill Cs
MOLECULAR AND CELLULAR BIOLOGY 2005;25(22):9845-58

Activin A and follicle-stimulating hormone control tight junctions in avian granulosa cells by regulating occludin expression
Schuster Mk, Schmierer B, Shkumatava A, Kuchler K
BIOLOGY OF REPRODUCTION 2004;70(5):1493-9

Analysis of Smad nucleocytoplasmic shuttling in living cells
Nicolas Fj, De Bosscher K, Schmierer B, Hill Cs
JOURNAL OF CELL SCIENCE 2004;117(18):4113-25

Activin and follicle-stimulating hormone signaling are required for long-term culture of functionally differentiated primary granulosa cells from the chicken ovary
Schmierer B, Schuster Mk, Shkumatava A, Kuchler K
BIOLOGY OF REPRODUCTION 2003;68(2):620-7

Activin A signaling induces Smad2, but not Smad3, requiring protein kinase A activity in granulosa cells from the avian ovary
Schmierer B, Schuster Mk, Shkumatava A, Kuchler K
JOURNAL OF BIOLOGICAL CHEMISTRY 2003;278(23):21197-203

Catecholamines up-regulate lipopolysaccharide-induced IL-6 production in human microvascular endothelial cells
Gornikiewicz A, Sautner T, Brostjan C, Schmierer B, Fugger R, Roth E, et al
FASEB JOURNAL 2000;14(9):1093-100

Endothelial cells downregulate expression of the 70 kDa heat shock protein during hypoxia
Oehler R, Schmierer B, Zellner M, Prohaska R, Roth E
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2000;274(2):542-7

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Events

SciLifeLab Science Summit

25 Apr 2018 - 09:00
Aula Medica, Nobels väg 6