I am heading High Throughput Genome Engineering, a National SciLifeLab Facility offering pooled lentiviral CRISPR/Cas9 screens to the Swedish research community.
Pooled CRISPR/Cas9 screening enables parallel interrogation of thousands of protein coding genes, lncRNAs or other genetic elements for involvement in biological processes of interest. HTGE provides access to verified lentiviral CRISPR guide libraries for whole genome and more targeted loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition, CRISPR activation). We also offer generation of stable Cas9-expressing lines in users’ cells of interest. HTGE supports the Swedish and international research community with CRISPR screening projects from planning to data analysis.
All our libraries are now barcoded, which is our own innovation and a major improvement compared to the conventional screening methodology (see our 2017 paper CRISPR/Cas9 screening using unique molecular identifiers).
Mathematical modeling identifies Smad nucleocytoplasmic shuttling as a dynamic signal-interpreting system
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008;105(18):6608-13
Two highly related regulatory subunits of PP2A exert opposite effects on TGF-beta/Activin/Nodal signalling
TGF beta-SMAD signal transduction: molecular specificity and functional flexibility
NATURE REVIEWS MOLECULAR CELL BIOLOGY 2007;8(12):970-82
Upregulation of chicken p15(INK4b) at senescence and in the developing brain
JOURNAL OF CELL SCIENCE 2006;119(12):2435-43
Kinetic analysis of smad nucleocytoplasmic shuttling reveals a mechanism for transforming growth factor beta-dependent nuclear accumulation of Smads
MOLECULAR AND CELLULAR BIOLOGY 2005;25(22):9845-58
Activin A and follicle-stimulating hormone control tight junctions in avian granulosa cells by regulating occludin expression
BIOLOGY OF REPRODUCTION 2004;70(5):1493-9
Analysis of Smad nucleocytoplasmic shuttling in living cells
JOURNAL OF CELL SCIENCE 2004;117(18):4113-25
Activin and follicle-stimulating hormone signaling are required for long-term culture of functionally differentiated primary granulosa cells from the chicken ovary
BIOLOGY OF REPRODUCTION 2003;68(2):620-7
Activin A signaling induces Smad2, but not Smad3, requiring protein kinase A activity in granulosa cells from the avian ovary
JOURNAL OF BIOLOGICAL CHEMISTRY 2003;278(23):21197-203
Catecholamines up-regulate lipopolysaccharide-induced IL-6 production in human microvascular endothelial cells
FASEB JOURNAL 2000;14(9):1093-100
Endothelial cells downregulate expression of the 70 kDa heat shock protein during hypoxia
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2000;274(2):542-7