Anna Pasetto

About me
Publications
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About me

I joined the Karolinska Institutet in 2018 as assistant professor (biträdande universitetslektor) and managing director of the pre-GMP facility in the department of Laboratory Medicine. I received my Master Degree in Molecular Biotechnology in 2007 from Alma Mater Studiorum Bologna University, Italy. I then moved to the Karolinska Institutet where I received my PhD in 2012. I joined the laboratory of Dr. Steven Rosenberg at the National Cancer Institute, NIH in 2013 and my research was focused on the isolation of TCR genes from mutation-reactive tumor infiltrating lymphocytes in metastatic solid cancers.

Research

My research goal is to develop new immunotherapies against cancer. The main focus is to identify immunological receptors able to recognize mutated antigens expressed by cancer, these receptors can then be engineered to generate CAR-T cells, TCR-T cells and other types of cell therapies.

Articles

Article: MOLECULAR THERAPY. 2024;32(2):540-555
Distinct roles of vaccine-induced SARS-CoV-2-specific neutralizing antibodies and T cells in protection and disease
Yan J; Bangalore CR; Nikouyan N; Appelberg S; Silva DN; Yao H; Pasetto A; Weber F; Weber S; Larsson O; Hoglund U; Bogdanovic G; Grabbe M; Aleman S; Szekely L; Szakos A; Tuvesson O; Gidlund E-K; Cadossi M; Salati S; Tegel H; Hober S; Frelin L; Mirazimi A; Ahlen G; Sallberg M
Article: NPJ PRECISION ONCOLOGY. 2024;8(1):26
Autologous anti-GD2 CAR T cells efficiently target primary human glioblastoma
Chiavelli C; Prapa M; Rovesti G; Silingardi M; Neri G; Pugliese G; Trudu L; Dall'Ora M; Golinelli G; Grisendi G; Vinet J; Bestagno M; Spano C; Papapietro RV; Depenni R; Di Emidio K; Pasetto A; Nascimento Silva D; Feletti A; Berlucchi S; Iaccarino C; Pavesi G; Dominici M
Article: GUT. 2023;72(6):1186-1195
Novel prime-boost immune-based therapy inhibiting both hepatitis B and D virus infections
Burm R; Maravelia P; Ahlen G; Ciesek S; Perez NC; Pasetto A; Urban S; Van Houtte F; Verhoye L; Wedemeyer H; Johansson M; Frelin L; Sallberg M; Meuleman P
Article: CELL REPORTS. 2022;40(5):111153
Cancer genes disfavoring T cell immunity identified via integrated systems approach
Kishton RJ; Patel SJ; Decker AE; Vodnala SK; Cam M; Yamamoto TN; Patel Y; Sukumar M; Yu Z; Ji M; Henning AN; Gurusamy D; Palmer DC; Stefanescu RA; Girvin AT; Lo W; Pasetto A; Malekzadeh P; Deniger DC; Wood KC; Sanjana NE; Restifo NP
Article: METHODS IN MOLECULAR BIOLOGY. 2022;2574:209-219
T-Cell Repertoire Characterization.
Pasetto A; Buggert M
Article: FRONTIERS IN IMMUNOLOGY. 2022;13:896242
Process Development for Adoptive Cell Therapy in Academia: A Pipeline for Clinical-Scale Manufacturing of Multiple TCR-T Cell Products
Silva DN; Chrobok M; Rovesti G; Healy K; Wagner AK; Maravelia P; Gatto F; Mazza M; Mazzotti L; Lohmann V; Chen MS; Sallberg M; Buggert M; Pasetto A
Article: JOURNAL OF INFECTIOUS DISEASES. 2021;223(1):128-138
Blocking Entry of Hepatitis B and D Viruses to Hepatocytes as a Novel Immunotherapy for Treating Chronic Infections
Maravelia P; Frelin L; Ni Y; Perez NC; Ahlen G; Jagya N; Verch G; Verhoye L; Pater L; Johansson M; Pasetto A; Meuleman P; Urban S; Sallberg M
Article: JOURNAL OF IMMUNOTHERAPY. 2021;44(1):1-8
Rapid Identification and Evaluation of Neoantigen-reactive T-Cell Receptors From Single Cells
Paria BC; Levin N; Lowery FJ; Pasetto A; Deniger DC; Parkhurst MR; Yossef R; Kim SP; Florentin M; Ngo LT; Ray S; Krishna S; Robbins PF; Rosenberg SA
Article: JOURNAL OF CLINICAL INVESTIGATION. 2019;129(11):4992-5004
Recognition of human gastrointestinal cancer neoantigens by circulating PD-1⁺ lymphocytes
Gros A; Tran E; Parkhurst MR; Ilyas S; Pasetto A; Groh EM; Robbins PF; Yossef R; Garcia-Garijo A; Fajardo CA; Prickett TD; Jia L; Gartner JJ; Ray S; Ngo L; Wunderllich JR; Yang JC; Rosenberg SA
Article: CANCER DISCOVERY. 2019;9(8):1022-1035
Unique Neoantigens Arise from Somatic Mutations in Patients with Gastrointestinal Cancers
Parkhurst MR; Robbins PF; Tran E; Prickett TD; Gartner JJ; Jia L; Ivey G; Li YF; El-Gamil M; Lalani A; Crystal JS; Sachs A; Groh E; Ray S; Ngo LT; Kivitz S; Pasetto A; Yossef R; Lowery FJ; Goff SL; Lo W; Cafri G; Deniger DC; Malekzadeh P; Ahmadzadeh M; Wunderlich JR; Somerville RPT; Rosenberg SA
Article: CANCER IMMUNOLOGY RESEARCH. 2019;7(4):534-543
Immunologic Recognition of a Shared p53 Mutated Neoantigen in a Patient with Metastatic Colorectal Cancer
Lo W; Parkhurst M; Robbins PF; Tran E; Lu Y-C; Jia L; Gartner JJ; Pasetto A; Deniger D; Malekzadeh P; Shelton TE; Prickett T; Ray S; Kivitz S; Paria BC; Kriley I; Schrump DS; Rosenberg SA
Article: JOURNAL OF CLINICAL INVESTIGATION. 2019;129(3):1109-1114
Neoantigen screening identifies broad TP53 mutant immunogenicity in patients with epithelial cancers
Malekzadeh P; Pasetto A; Robbins PF; Parkhurst MR; Paria BC; Jia L; Gartner JJ; Hill V; Yu Z; Restifo NP; Sachs A; Iran E; Lo W; Somerville RPT; Rosenberg SA; Deniger DC
Article: NATURE COMMUNICATIONS. 2019;10(1):449
Memory T cells targeting oncogenic mutations detected in peripheral blood of epithelial cancer patients
Cafri G; Yossef R; Pasetto A; Deniger DC; Lu Y-C; Parkhurst M; Gartner JJ; Jia L; Ray S; Ngo LT; Jafferji M; Sachs A; Prickett T; Robbins PF; Rosenberg SA
Article: SCIENCE IMMUNOLOGY. 2019;4(31):eaao4310
Tumor-infiltrating human CD4⁺ regulatory T cells display a distinct TCR repertoire and exhibit tumor and neoantigen reactivity
Ahmadzadeh M; Pasetto A; Jia L; Deniger DC; Stevanovic S; Robbins PF; Rosenberg SA
Article: CLINICAL CANCER RESEARCH. 2018;24(22):5562-5573
T-cell Responses to TP53 "Hotspot" Mutations and Unique Neoantigens Expressed by Human Ovarian Cancers
Deniger DC; Pasetto A; Robbins PF; Gartner JJ; Prickett TD; Paria BC; Malekzadeh P; Jia L; Yossef R; Langhan MM; Wunderlich JR; Danforth DN; Somerville RPT; Rosenberg SA
Article: JCI INSIGHT. 2018;3(19):122467
Enhanced detection of neoantigen-reactive T cells targeting unique and shared oncogenes for personalized cancer immunotherapy
Yossef R; Tran E; Deniger DC; Gros A; Pasetto A; Parkhurst MR; Gartner JJ; Prickett TD; Cafri G; Robbins PF; Rosenberg SA
Article: NATURE MEDICINE. 2018;24(6):724-730
Immune recognition of somatic mutations leading to complete durable regression in metastatic breast cancer
Zacharakis N; Chinnasamy H; Black M; Xu H; Lu Y-C; Zheng Z; Pasetto A; Langhan M; Shelton T; Prickett T; Gartner J; Jia L; Trebska-McGowan K; Somerville RP; Robbins PF; Rosenberg SA; Goff SL; Feldman SA
Article: CLINICAL CANCER RESEARCH. 2017;23(10):2491-2505
Isolation of T-Cell Receptors Specifically Reactive with Mutated Tumor-Associated Antigens from Tumor-Infiltrating Lymphocytes Based on CD137 Expression
Parkhurst M; Gros A; Pasetto A; Prickett T; Crystal JS; Robbins P; Rosenberg SA
Article: JOURNAL OF VIROLOGY. 2017;91(9):e00010-e00017
Hepatitis C Virus-Specific T Cell Receptor mRNA-Engineered Human T Cells: Impact of Antigen Specificity on Functional Properties
Balasiddaiah A; Davanian H; Aleman S; Pasetto A; Frelin L; Sallberg M; Lohmann V; Koh S; Bertoletti A; Chen M
Article: SCIENCE. 2017;356(6334):200-205
Landscape of immunogenic tumor antigens in successful immunotherapy of virally induced epithelial cancer
Stevanovic S; Pasetto A; Helman SR; Gartner JJ; Prickett TD; Howie B; Robins HS; Robbins PF; Klebanoff CA; Rosenberg SA; Hinrichs CS
Article: CLINICAL CANCER RESEARCH. 2017;23(2):351-362
A Pilot Trial of the Combination of Vemurafenib with Adoptive Cell Therapy in Patients with Metastatic Melanoma
Deniger DC; Kwong MLM; Pasetto A; Dudley ME; Wunderlich JR; Langhan MM; Lee C-CR; Rosenberg SA
Article: NEW ENGLAND JOURNAL OF MEDICINE. 2016;375(23):2255-2262
T-Cell Transfer Therapy Targeting Mutant KRAS in Cancer
Tran E; Robbins PF; Lu Y-C; Prickett TD; Gartner JJ; Jia L; Pasetto A; Zheng Z; Ray S; Groh EM; Kriley IR; Rosenberg SA
Article: CANCER IMMUNOLOGY RESEARCH. 2016;4(9):734-743
Tumor- and Neoantigen-Reactive T-cell Receptors Can Be Identified Based on Their Frequency in Fresh Tumor
Pasetto A; Gros A; Robbins PF; Deniger DC; Prickett TD; Matus-Nicodemos R; Douek DC; Howie B; Robins H; Parkhurst MR; Gartner J; Trebska-McGowan K; Crystal JS; Rosenberg SA
Article: CANCER IMMUNOLOGY RESEARCH. 2016;4(8):669-678
Durable Complete Response from Metastatic Melanoma after Transfer of Autologous T Cells Recognizing 10 Mutated Tumor Antigens
Prickett TD; Crystal JS; Cohen CJ; Pasetto A; Parkhurst MR; Gartner JJ; Yao X; Wang R; Gros A; Li YF; El-Gamil M; Trebska-McGowan K; Rosenberg SA; Robbins PF
Article: MOLECULAR THERAPY. 2016;24(6):1078-1089
Stable, Nonviral Expression of Mutated Tumor Neoantigen-specific T-cell Receptors Using the Sleeping Beauty Transposon/Transposase System
Deniger DC; Pasetto A; Tran E; Parkhurst MR; Cohen CJ; Robbins PF; Cooper LJN; Rosenberg SA
Article: NATURE MEDICINE. 2016;22(4):433-438
Prospective identification of neoantigen-specific lymphocytes in the peripheral blood of melanoma patients
Gros A; Parkhurst MR; Tran E; Pasetto A; Robbins PF; Ilyas S; Prickett TD; Gartner JJ; Crystal JS; Roberts IM; Trebska-McGowan K; Wunderlich JR; Yang JC; Rosenberg SA
Article: JOURNAL OF IMMUNOLOGY. 2013;190(3):1113-1124
A Synthetic Codon-Optimized Hepatitis C Virus Nonstructural 5A DNA Vaccine Primes Polyfunctional CD8⁺ T Cell Responses in Wild-Type and NS5A-Transgenic Mice
Holmstrom F; Pasetto A; Nahr V; Brass A; Kriegs M; Hildt E; Broderick KE; Chen M; Ahlen G; Frelin L
Article: PLOS ONE. 2013;8(6):e65964
The Adjuvant Activity of Alphavirus Replicons Is Enhanced by Incorporating the Microbial Molecule Flagellin into the Replicon
Knudsen ML; Johansson DX; Kostic L; Nordstron EKL; Tegerstedt K; Pasetto A; Applequist SE; Ljungberg K; Sirard J-C; Liljestrom P
Article: JOURNAL OF IMMUNOLOGY. 2012;189(9):4510-4519
TCR-Redirected Human T Cells Inhibit Hepatitis C Virus Replication: Hepatotoxic Potential Is Linked to Antigen Specificity and Functional Avidity
Pasetto A; Frelin L; Aleman S; Holmstrom F; Brass A; Ahlen G; Brenndorfer ED; Lohmann V; Bartenschlager R; Sallberg M; Bertoletti A; Chen M
Article: JOURNAL OF GENERAL VIROLOGY. 2012;93(Pt 2):247-258
Generation of T-cell receptors targeting a genetically stable and immunodominant cytotoxic T-lymphocyte epitope within hepatitis C virus non-structural protein 3
Pasetto A; Frelin L; Brass A; Yasmeen A; Koh S; Lohmann V; Bartenschlager R; Magalhaes I; Maeurer M; Sallberg M; Chen M
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All other publications

Editorial comment: FRONTIERS IN ONCOLOGY. 2023;13:1171907
Personalized immunotherapy for cancer
Pasetto A; Lu Y-C
Review: IMMUNO-ONCOLOGY AND TECHNOLOGY. 2022;16:100099
ATMP development and pre-GMP environment in academia: a safety net for early cell and gene therapy development and manufacturing.
Silva DN; Chrobok M; Ahlén G; Blomberg P; Sällberg M; Pasetto A
Review: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2022;23(15):8590
T-Cell Receptor Repertoire Sequencing and Its Applications: Focus on Infectious Diseases and Cancer
Mazzotti L; Gaimari A; Bravaccini S; Maltoni R; Cerchione C; Juan M; Navarro EA-G; Pasetto A; Nascimento Silva D; Ancarani V; Sambri V; Calabro L; Martinelli G; Mazza M
Review: CANCERS. 2021;13(17):4334
Liquid Biopsy in Hepatocellular Carcinoma: Opportunities and Challenges for Immunotherapy
Maravelia P; Silva DN; Rovesti G; Chrobok M; Stal P; Lu Y-C; Pasetto A
Review: FRONTIERS IN IMMUNOLOGY. 2021;12:689091
Single-Cell TCR and Transcriptome Analysis: An Indispensable Tool for Studying T-Cell Biology and Cancer Immunotherapy
Pasetto A; Lu Y-C
Editorial comment: CANCERS. 2020;12(10):E2818-2818
Identification and Clinical Application of Immunological Receptors Targeting Mutated Antigens Expressed by Solid Tumors
Pasetto A
Review: CELLS. 2020;9(6):E1471-1471
Chronic Viral Liver Diseases: Approaching the Liver Using T Cell Receptor-Mediated Gene Technologies
Healy K; Pasetto A; Sobkowiak MJ; Soon CF; Cornberg M; Aleman S; Chen MS
Review: FRONTIERS IN IMMUNOLOGY. 2020;11:552
Liver, Tumor and Viral Hepatitis: Key Players in the Complex Balance Between Tolerance and Immune Activation
Sallberg M; Pasetto A
Editorial comment: ALIMENTARY PHARMACOLOGY AND THERAPEUTICS. 2019;50(8):963-964
Editorial: HBV-the naked truth?
Pasetto A; Ahlen G; Sallberg M
Review: ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS. 2014;62(1):23-30
Functional Attributes of Responding T Cells in HCV Infection: The Recent Advances in Engineering Functional Antiviral T Cells
Pasetto A; Aleman S; Chen M

Employments

Research Specialist, Department of Laboratory Medicine, Karolinska Institutet, 2022-

Degrees and Education

Degree Of Doctor Of Philosophy, Department of Dental Medicine, Karolinska Institutet, 2012

About me

Research

Articles

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Degrees and Education

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