Amanda Andersson Rolf

I am a stem cell biologist, fascinated by how stem cells play a key role not only during development but also in regeneration and disease. I enjoy research happening at the intersection of different fields – such as the combination of engineering precision with biological discovery.

My scientific journey began with a focus on tool development: during my PhD, I engineered advanced genetic tools to dissect gene function in both embryonic and adult stem cells. Eager to understand how these cells coordinate to build complex tissues, I shifted my focus to organogenesis. As a postdoctoral fellow, I developed a pioneering human fetal pancreatic organoid model. This model was first to support long-term maintenance of human tripotent stem cells allowing simultaneous presence of acinar, ductal, and endocrine lineages - offering an in vitro model for studying human pancreas cell identity, lineage specification and organogenesis.

Investigating the mechanisms regulating cell identity in development to promote regeneration and prevent disease.

We explore how cell identity is established and maintained during endoderm organ development and regeneration - uncovering how this fundamental process not only build and re-build tissues but also how its deregulation can drive and contribute to disease progression.

We apply an interdisciplinary approach integrating organoid technology (pancreas and stomach), (spatial)omics, genome- and bio-engineering. These technologies enable us to analyse individual cells within their native tissue context (including human primary tissue samples) and perform functional studies on human in vitro model.

Do you want to join our research group?

Curious and motivated people are always welcome to reach out! If you are interested and want to know more/join our mission, feel free to reach out to amanda.andersson.rolf@ki.se.