Alda Saldan

About me
Publications
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About me

I am a flow cytometry research engineer at the Biomedicum Flow Cytometry Facility at KI. I have experience in conventional &
spectral flow cytometry, fluorescence-activated cell sorting (FACS), and high-parameter flow cytometry data analysis.

My experience and skills cover the following techniques and assays:
- In-depth immunophenotyping of myeloid and lymphoid cell subsets in human and mouse samples
- Cell cycle and proliferation analysis
- Cytotoxicity &
immune killing assays
- Cytokine Bead Array
- Cell trafficking and co-localization by image cytometry
- Data analysis on different software/platforms (FACSDiva, FlowJo, FlowLogic, OMIQ, and more)
- FACS sorting (eukaryotic cells, nuclei, bacteria)
- Education &
training for researchers on conventional &
spectral flow cytometry
- Assistance with panel design and data analysis for both conventional and spectral flow cytometry.
If you are planning to use flow cytometry or FACS for your research, you are welcome to contact me for advice or for any flow cytometry-related question.

EDUCATION
BSc (Honours) in Molecular Biology (2007, University of Padova, Italy)
MSc in Health Biology (2009, University of Padova, Italy)
Postgraduate Specialisation Degree in Clinical Microbiology and Virology (2015, University of Torino, Italy)
PhD in Immunology (2021, The University of Queensland, Australia)

Research

Before joining the Biomedicum FACS Facility (BFC), my research activity has been focused mostly on the evaluation of the cytomegalovirus (CMV)-specific immune responses in immunocompromised patients and in studying the interplay between CMV infection and the anti-leukaemic immune responses after allogeneic hematopoietic stem cell transplantation.

Articles

Article: JOURNAL OF CLINICAL INVESTIGATION. 2024;134(7):e174184

IL-6-mediated endothelial injury impairs antiviral humoral immunity after bone marrow transplantation.

Zhang P; Fleming P; Andoniou CE; Waltner OG; Bhise SS; Martins JP; McEnroe BA; Voigt V; Daly S; Kuns RD; Ekwe AP; Henden AS; Saldan A; Olver S; Varelias A; Smith C; Schmidt CR; Ensbey KS; Legg SR; Sekiguchi T; Minnie SA; Gradwell M; Wagenaar I; Clouston AD; Koyama M; Furlan SN; Kennedy GA; Ward ES; Degli-Esposti MA; Hill GR; Tey S-K
Article: CYTOTHERAPY. 2024;:S1465-3249(24)00066-5

Clinical grade multiparametric cell sorting and gene-marking of regulatory T cells.

Ekwe AP; Au R; Zhang P; McEnroe BA; Tan ML; Saldan A; Henden AS; Hutchins CJ; Henderson A; Mudie K; Kerr K; Fuery M; Kennedy GA; Hill GR; Tey S-K
Article: FRONTIERS IN IMMUNOLOGY. 2023;14:1171197

Human cytomegalovirus and Epstein-Barr virus infections occurring early after transplantation are risk factors for antibody-mediated rejection in heart transplant recipients.

Saldan A; Mengoli C; Sgarabotto D; Fedrigo M; Angelini A; Feltrin G; Gambino A; Gerosa G; Barzon L; Abate D
Article: VIRUSES-BASEL. 2022;15(1):112

Four-Year Follow-Up of the Maternal Immunological, Virological and Clinical Settings of a 36-Year-Old Woman Experiencing Primary Cytomegalovirus Infection Leading to Intrauterine Infection.

Forner G; Saldan A; Mengoli C; Pizzi S; Fedrigo M; Gussetti N; Visentin S; Angelini A; Cosmi E; Barzon L; Abate DA
Article: JOURNAL OF CLINICAL MICROBIOLOGY. 2016;54(8):2149-2154

Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot Assay but Not CMV QuantiFERON Assay Is a Novel Biomarker To Determine Risk of Congenital CMV Infection in Pregnant Women.

Forner G; Saldan A; Mengoli C; Gussetti N; Palù G; Abate D
Article: JOURNAL OF CLINICAL MICROBIOLOGY. 2016;54(5):1352-1356

Comparison of the Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot and CMV QuantiFERON Cell-Mediated Immune Assays in CMV-Seropositive and -Seronegative Pregnant and Nonpregnant Women.

Saldan A; Forner G; Mengoli C; Tinto D; Fallico L; Peracchi M; Gussetti N; Palù G; Abate D
Article: CLINICAL INFECTIOUS DISEASES. 2015;61(8):1228-1234

Strong Cell-Mediated Immune Response to Human Cytomegalovirus Is Associated With Increased Risk of Fetal Infection in Primarily Infected Pregnant Women.

Saldan A; Forner G; Mengoli C; Gussetti N; Palù G; Abate D
Article: DRUG DEVELOPMENT RESEARCH. 2014;75 Suppl 1:S73-S76

Effects of TNF-alpha inhibitors on circulating Th17 cells in patients affected by severe psoriasis.

Piaserico S; Sandini E; Saldan A; Abate D
Journal article: JOURNAL OF CLINICAL MICROBIOLOGY. 2014;52(3):1025

Comparison of Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot and CMV Quantiferon Gamma Interferon-Releasing Assays in Assessing Risk of CMV Infection in Kidney Transplant Recipients (vol 51, pg 2501, 2013)

Abate D; Saldan A; Mengoli C; Fiscon M; Silvestre C; Fallico L; Peracchi M; Furian L; Cusinato R; Bonfante L; Rossi B; Marchini F; Sgarabotto D; Rigotti P; Palu G
Article: JOURNAL OF VIROLOGICAL METHODS. 2014;196:157-162

Optimization of interferon gamma ELISPOT assay to detect human cytomegalovirus specific T-cell responses in solid organ transplants.

Abate D; Saldan A; Forner G; Tinto D; Bianchin A; Palù G
Article: JOURNAL OF CLINICAL MICROBIOLOGY. 2013;51(8):2501-2507

Comparison of cytomegalovirus (CMV) enzyme-linked immunosorbent spot and CMV quantiferon gamma interferon-releasing assays in assessing risk of CMV infection in kidney transplant recipients.

Abate D; Saldan A; Mengoli C; Fiscon M; Silvestre C; Fallico L; Peracchi M; Furian L; Cusinato R; Bonfante L; Rossi B; Marchini F; Sgarabotto D; Rigotti P; Palù G
Article: WORLD J VIROL. 2012;1(6):150-153

Evaluation of virus-specific cellular immune response in transplant patients.

Costa C; Saldan A; Cavallo R
Article: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. 2012;25(4):1003-1009

The lack of cytomegalovirus-specific cellular immune response may contribute to the onset of organ infection and disease in lung transplant recipients.

Costa C; Saldan A; Sinesi F; Sidoti F; Balloco C; Simeone S; Piceghello A; Mantovani S; Di Nauta A; Solidoro P; Cavallo R
Article: CLINICAL MICROBIOLOGY AND INFECTION. 2012;18(8):E305-E307

Clinical impact of HSV-1 detection in the lower respiratory tract from hospitalized adult patients.

Costa C; Sidoti F; Saldan A; Sinesi F; Balloco C; Simeone S; Lorusso M; Mantovani S; Merlino C; Solidoro P; Cavallo R
Article: JOURNAL OF CLINICAL MICROBIOLOGY. 2012;50(6):1974-1980

Human cytomegalovirus-specific T-cell immune reconstitution in preemptively treated heart transplant recipients identifies subjects at critical risk for infection.

Abate D; Fiscon M; Saldan A; Cofano S; Mengoli C; Sgarabotto D; d'Agostino C; Barzon L; Cusinato R; Toscano G; Feltrin G; Gambino A; Gerosa G; Palù G
Article: TRANSPLANTATION. 2012;93(5):536-542

Diagnostic utility of human cytomegalovirus-specific T-cell response monitoring in predicting viremia in pediatric allogeneic stem-cell transplant patients.

Abate D; Cesaro S; Cofano S; Fiscon M; Saldan A; Varotto S; Mengoli C; Pillon M; Calore E; Biasolo MA; Cusinato R; Barzon L; Messina C; Carli M; Palù G
Article: JOURNAL OF INFECTIOUS DISEASES. 2010;202(4):585-594

Evaluation of cytomegalovirus (CMV)-specific T cell immune reconstitution revealed that baseline antiviral immunity, prophylaxis, or preemptive therapy but not antithymocyte globulin treatment contribute to CMV-specific T cell reconstitution in kidney transplant recipients.

Abate D; Saldan A; Fiscon M; Cofano S; Paciolla A; Furian L; Ekser B; Biasolo MA; Cusinato R; Mengoli C; Bonfante L; Rossi B; Rigotti P; Sgarabotto D; Barzon L; Palù G

All other publications

Meeting abstract: BLOOD. 2021;138(Supplement 1):648

Early Cytomegalovirus Reactivation after Allogenic Bone Marrow Transplantation Is Associated with the Loss of Recipient-Derived Humoral Immunity and Is Reduced By IL-6 Inhibition

Zhang P; Fleming P; Andoniou C; Martins JP; Saldan A; Kuns R; McEnroe B; Henden AS; Olver S; Ekwe A; Varelias A; Smith C; Schmidt CR; Ensbey KS; Boeckh M; Kennedy GA; Degli-Esposti M; Hill GR; Tey S-K
Meeting abstract: TRANSPLANTATION. 2018;102(&NA;):S334

The Efficacy of CMV Specific Immune Reconstitution is Highly Dependent on Donor/Recipient Serostatus and Type of Solid Organ Transplanted

Saldan A; Mengoli C; Sgarabotto D; Alessi M; Gambino A; Bonfante L; Burra P; Marchini F; Rossi B; Boccagni P; Fraiese AP; Toscano G; Fasolato S; Silvestre C; Russo FP; Angeli P; Furian L; Loy M; Sturniolo G; Cillo U; Gerosa G; Rigotti P; Rea F; Palù G; Abate D
Review: JOURNAL OF CLINICAL MICROBIOLOGY. 2017;55(3):693-702

Testing for Cytomegalovirus in Pregnancy.

Saldan A; Forner G; Mengoli C; Gussetti N; Palù G; Abate D

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