Alda Saldan

About me
Publications
Public outreach and news

About me

I am a flow cytometry research engineer at the Biomedicum Flow Cytometry Facility at KI. I have experience in conventional &
spectral flow cytometry, fluorescence-activated cell sorting (FACS), and high-parameter flow cytometry data analysis.

My experience and skills cover the following techniques and assays:
- In-depth immunophenotyping of myeloid and lymphoid cell subsets in human and mouse samples
- Cell cycle and proliferation analysis
- Cytotoxicity &
immune killing assays
- Cytokine Bead Array
- Cell trafficking and co-localization by image cytometry
- Data analysis on different software/platforms (FACSDiva, FlowJo, FlowLogic, OMIQ, and more)
- FACS sorting (eukaryotic cells, nuclei, bacteria)
- Education &
training for researchers on conventional &
spectral flow cytometry
- Assistance with panel design and data analysis for both conventional and spectral flow cytometry.
If you are planning to use flow cytometry or FACS for your research, you are welcome to contact me for advice or for any flow cytometry-related question.

EDUCATION
BSc (Honours) in Molecular Biology (2007, University of Padova, Italy)
MSc in Health Biology (2009, University of Padova, Italy)
Postgraduate Specialisation Degree in Clinical Microbiology and Virology (2015, University of Torino, Italy)
PhD in Immunology (2021, The University of Queensland, Australia)

Research

Before joining the Biomedicum FACS Facility (BFC), my research activity has been focused mostly on the evaluation of the cytomegalovirus (CMV)-specific immune responses in immunocompromised patients and in studying the interplay between CMV infection and the anti-leukaemic immune responses after allogeneic hematopoietic stem cell transplantation.

Articles

Article: CYTOTHERAPY. 2024;26(7):719-728
Clinical grade multiparametric cell sorting and gene-marking of regulatory T cells
Ekwe AP; Au R; Zhang P; Mcenroe BA; Tan ML; Saldan A; Henden AS; Hutchins CJ; Henderson A; Mudie K; Kerr K; Fuery M; Kennedy GA; Hill GR; Tey S-K
Article: JOURNAL OF CLINICAL INVESTIGATION. 2024;134(7):e174184
IL-6-mediated endothelial injury impairs antiviral humoral immunity after bone marrow transplantation
Zhang P; Fleming P; Andoniou CE; Waltner OG; Bhise SS; Martins JP; McEnroe BA; Voigt V; Daly S; Kuns RD; Ekwe AP; Henden AS; Saldan A; Olver S; Varelias A; Smith C; Schmidt CR; Ensbey KS; Legg SRW; Sekiguchi T; Minnie SA; Gradwell M; Wagenaar I; Clouston AD; Koyama M; Furlan SN; Kennedy GA; Ward ES; Degli-Esposti MA; Hill GR; Tey S-K
Article: FRONTIERS IN IMMUNOLOGY. 2023;14:1171197
Human cytomegalovirus and Epstein-Barr virus infections occurring early after transplantation are risk factors for antibody-mediated rejection in heart transplant recipients.
Saldan A; Mengoli C; Sgarabotto D; Fedrigo M; Angelini A; Feltrin G; Gambino A; Gerosa G; Barzon L; Abate D
Article: VIRUSES. 2022;15(1):112
Four-Year Follow-Up of the Maternal Immunological, Virological and Clinical Settings of a 36-Year-Old Woman Experiencing Primary Cytomegalovirus Infection Leading to Intrauterine Infection
Forner G; Saldan A; Mengoli C; Pizzi S; Fedrigo M; Gussetti N; Visentin S; Angelini A; Cosmi E; Barzon L; Abate DA
Article: JOURNAL OF CLINICAL MICROBIOLOGY. 2016;54(8):2149-2154
Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot Assay but Not CMV QuantiFERON Assay Is a Novel Biomarker To Determine Risk of Congenital CMV Infection in Pregnant Women
Forner G; Saldan A; Mengoli C; Gussetti N; Palu G; Abate D
Article: JOURNAL OF CLINICAL MICROBIOLOGY. 2016;54(5):1352-1356
Comparison of the Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot and CMV QuantiFERON Cell-Mediated Immune Assays in CMV-Seropositive and -Seronegative Pregnant and Nonpregnant Women
Saldan A; Forner G; Mengoli C; Tinto D; Fallico L; Peracchi M; Gussetti N; Palu G; Abate D
Article: CLINICAL INFECTIOUS DISEASES. 2015;61(8):1228-1234
Strong Cell-Mediated Immune Response to Human Cytomegalovirus Is Associated With Increased Risk of Fetal Infection in Primarily Infected Pregnant Women
Saldan A; Forner G; Mengoli C; Gussetti N; Palu G; Abate D
Article: DRUG DEVELOPMENT RESEARCH. 2014;75:S73-S76
Effects of TNF-Alpha Inhibitors on Circulating Th17 Cells in Patients Affected by Severe Psoriasis
Piaserico S; Sandini E; Saldan A; Abate D
Journal article: JOURNAL OF CLINICAL MICROBIOLOGY. 2014;52(3):1025
Comparison of Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot and CMV Quantiferon Gamma Interferon-Releasing Assays in Assessing Risk of CMV Infection in Kidney Transplant Recipients (vol 51, pg 2501, 2013)
Abate D; Saldan A; Mengoli C; Fiscon M; Silvestre C; Fallico L; Peracchi M; Furian L; Cusinato R; Bonfante L; Rossi B; Marchini F; Sgarabotto D; Rigotti P; Palu G
Article: JOURNAL OF VIROLOGICAL METHODS. 2014;196:157-162
Optimization of interferon gamma ELISPOT assay to detect human cytomegalovirus specific T-cell responses in solid organ transplants
Abate D; Saldan A; Forner G; Tinto D; Bianchin A; Palu G
Article: JOURNAL OF CLINICAL MICROBIOLOGY. 2013;51(8):2501-2507
Comparison of Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot and CMV Quantiferon Gamma Interferon-Releasing Assays in Assessing Risk of CMV Infection in Kidney Transplant Recipients
Abate D; Saldan A; Mengoli C; Fiscon M; Silvestre C; Fallico L; Peracchi M; Furian L; Cusinato R; Bonfante L; Rossi B; Marchini F; Sgarabotto D; Rigotti P; Palu G
Article: WORLD J VIROL. 2012;1(6):150-153
Evaluation of virus-specific cellular immune response in transplant patients.
Costa C; Saldan A; Cavallo R
Article: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. 2012;25(4):1003-1009
THE LACK OF CYTOMEGALOVIRUS-SPECIFIC CELLULAR IMMUNE RESPONSE MAY CONTRIBUTE TO THE ONSET OF ORGAN INFECTION AND DISEASE IN LUNG TRANSPLANT RECIPIENTS
Costa C; Saldan A; Sinesi F; Sidoti F; Balloco C; Simeone S; Piceghello A; Mantovani S; Di Nauta A; Solidoro P; Cavallo R
Article: CLINICAL MICROBIOLOGY AND INFECTION (CMI). 2012;18(8):E305-E307
Clinical impact of HSV-1 detection in the lower respiratory tract from hospitalized adult patients
Costa C; Sidoti F; Saldan A; Sinesi F; Balloco C; Simeone S; Lorusso M; Mantovani S; Merlino C; Solidoro P; Cavallo R
Article: JOURNAL OF CLINICAL MICROBIOLOGY. 2012;50(6):1974-1980
Human Cytomegalovirus-Specific T-Cell Immune Reconstitution in Preemptively Treated Heart Transplant Recipients Identifies Subjects at Critical Risk for Infection
Abate D; Fiscon M; Saldan A; Cofano S; Mengoli C; Sgarabotto D; d'Agostino C; Barzon L; Cusinato R; Toscano G; Feltrin G; Gambino A; Gerosa G; Palu G
Article: TRANSPLANTATION. 2012;93(5):536-542
Diagnostic Utility of Human Cytomegalovirus-Specific T-Cell Response Monitoring in Predicting Viremia in Pediatric Allogeneic Stem-Cell Transplant Patients
Abate D; Cesaro S; Cofano S; Fiscon M; Saldan A; Varotto S; Mengoli C; Pillon M; Calore E; Biasolo MA; Cusinato R; Barzon L; Messina C; Carli M; Palu G
Article: JOURNAL OF INFECTIOUS DISEASES. 2010;202(4):585-594
Evaluation of Cytomegalovirus (CMV)-Specific T Cell Immune Reconstitution Revealed That Baseline Antiviral Immunity, Prophylaxis, or Preemptive Therapy but not Antithymocyte Globulin Treatment Contribute to CMV-Specific T Cell Reconstitution in Kidney Transplant Recipients
Abate D; Saldan A; Fiscon M; Cofano S; Paciolla A; Furian L; Ekser B; Biasolo MA; Cusinato R; Mengoli C; Bonfante L; Rossi B; Rigotti P; Sgarabotto D; Barzon L; Palu G

All other publications

Meeting abstract: BLOOD. 2021;138:648
Early Cytomegalovirus Reactivation after Allogenic Bone Marrow Transplantation Is Associated with the Loss of Recipient-Derived Humoral Immunity and Is Reduced By IL-6 Inhibition
Zhang P; Fleming P; Andoniou C; Martins JP; Saldan A; Kuns R; McEnroe B; Henden AS; Olver S; Ekwe A; Varelias A; Smith C; Schmidt CR; Ensbey KS; Boeckh M; Kennedy GA; Degli-Esposti M; Hill GR; Tey SK
Meeting abstract: TRANSPLANTATION. 2018;102:S334
The Efficacy of CMV Specific Immune Reconstitution is Highly Dependent on Donor/Recipient Serostatus and Type of Solid Organ Transplanted
Saldan A; Mengoli C; Sgarabotto D; Alessi M; Gambino A; Bonfante L; Burra P; Marchini F; Rossi B; Boccagni P; Fraiese AP; Toscano G; Fasolato S; Silvestre C; Russo FP; Angeli P; Furian L; Loy M; Sturniolo G; Cillo U; Gerosa G; Rigotti P; Rea F; Palu G; Abate D
Review: JOURNAL OF CLINICAL MICROBIOLOGY. 2017;55(3):693-702
Testing for Cytomegalovirus in Pregnancy.
Saldan A; Forner G; Mengoli C; Gussetti N; Palù G; Abate D

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