Pluripotency, Embryonic Stem Cells and Regenerative Medicine
The Lanner lab aims to:
1. Uncover the fundamental principles underlying how the first cell types and pluripotency is established in the early human embryo.
2. Establish a good manufacturing practice (GMP) bank of clinically compliant high quality embryonic stem cells (ESCs).
3. Explore treatment of age-related macula degeneration using ESC derived retinal pigmented epithelial cells.
ESCs isolated from the human blastocyst can be propagated indefinitely and directed into any cell type of the adult human, offering great potential for regenerative medicine to replace lost or damaged tissues. The knowledge of how these pluripotent cells are established and controlled in vivo is generated primarily in animal modes such as the mouse while our knowledge of the same process in the human is rudimentary and key differences are emerging. We will explore these issues through single cell mRNA sequencing, pioneering genetic technologies and functional assays in human blastocysts. This fundamental knowledge will also be important for our understanding of infertility, a problem affecting 10-15% of all couples in our society.
We have established an efficient differentiation methodology of ESCs into clinically relevant cell types. However, before these safely can become clinical practice, additional hurdles must be overcome such as GMP production and immune matching of cells and patients. By establishing the KI Stem Cell Bank we hope to resolve these issues and facilitate efforts to move stem cell based regenerative medicine into the clinic. Finally, we will specifically aim to initiate trials based on ESC in treatment of macular degeneration, the major cause of blindness in our elderly population.
Current Lab members
Dr. Liselotte Antonsson, Lab manager
Dr. Sophie Petroploulos, Postdoc. Single cell RNA sequencing analysis of human blastocyst formation.
Dr. Sebastian Hildebrand, Postdoc. Establishing the biophysical properties of the trofectoderm, the first epithelial celltype.
Dr. Sarita Pauliina Panula, Postdoc. Establishing novel tools to explore distinct stem cell types in the human embryo.
Dr. Pankaj Kumar, Postdoc. Bioinformatics of pluripotency and early human development.
John Paul Schell, PhD student. Dissecting naive and primed pluripotency.
Alvaro Plaza Reyes, PhD student. Treating AMD using ESCs. Establishing genetic technologies to study early human development.
Sara Padrell Sánchez, PhD student. Treating AMD using ESCs.
Nerges Winblad, Research assistant. Establishing genetic technologies to study early human development.
Interested in joining the lab?
Applications for post-doc positions are very welcomed if the applicant can bring financing. Otherwise early contacts are encouraged to plan applications for external personal post-doc grants.
KI Stem Cell Bank
Together with Liselotte Antonsson and Mona Hedenskog we are working intensively to derive clinically compliant GMP ESCs which we will be usefull for academic and industial efforts within stem cell based regenerative medicin.
Petrus-Reurer S, Bartuma H, Aronsson M, Westman S, Lanner F, André H, Kvanta A. Invest Ophthalmol Vis Sci. 2017 Feb 1;58(2):1314-1322. doi: 10.1167/iovs.16-20738.
Posfai E, Petropoulos S, de Barros FR, Schell JP, Jurisica I, Sandberg R, Lanner F, Rossant J. Elife. 2017 Feb 22;6. pii: e22906. doi: 10.7554/eLife.22906.
Plaza Reyes A. and Lanner F. Development 2017 144: 3-7; doi: 10.1242/dev.139683
Faridani OR, Abdullayev I, Hagemann-Jensen M, Schell JP, Lanner F, Sandberg R. Nat Biotechnol. 2016 Oct 31. doi: 10.1038/nbt.3701.
Chen G, Schell JP, Benitez JA, Petropoulos S, Yilmaz M, Reinius B, Alekseenko Z, Shi L, Hedlund E, Lanner F, Sandberg R, Deng Q.
Genome Res. 2016 Aug 2. pii: gr.201954.115. [Epub ahead of print]
Madissoon E, Jouhilahti EM, Vesterlund L, Töhönen V, Krjutškov K, Petropoulous S, Einarsdottir E, Linnarsson S, Lanner F, Månsson R, Hovatta O, Bürglin TR, Katayama S, Kere J.
Sci Rep. 2016 Jul 14;6:28995. doi: 10.1038/srep28995.
Single-cell RNA-seq reveals lineage and X-chromosome dynamics in human preimplantation embryos
Sophie Petropoulos, Daniel Edsgärd, Björn Reinius, Qiaolin Deng, Sarita Pauliina Panula, Simone Codeluppi, Alvaro Plaza Reyes, Sten Linnarsson, Rickard Sandberg, Fredrik Lanner
Cell, published online 7 April 2016, doi: 10.1016/j.cell.2016.03.023
Petropoulos S, Panula SP, Schell JP, Lanner F.
J Intern Med. 2016 Apr 5. doi: 10.1111/joim.12493.
Alvaro Plaza Reyes, Sandra Petrus-Reurer, Liselotte Antonsson, Sonya Stenfelt, Hammurabi Bartuma, Sarita Panula, Theresa Mader, Iyadh Douagi, Helder André, Outi Hovatta, Fredrik Lanner, Anders Kvanta.
Stem Cell Reports, online 24 december 2015
Zhang H, Panula S, Petropoulos S, Edsgärd D, Busayavalasa K, Liu L, Li X, Risal S, Shen Y, Shao J, Liu M, Li S, Zhang D, Zhang X, Gerner RR, Sheikhi M, Damdimopoulou P, Sandberg R, Douagi I, Gustafsson JÅ, Liu L, Lanner F, Hovatta O, Liu K.
Nat Med. 2015 Oct 7;21(10):1116-8
Exp Cell Res. 2014 Feb 1;321(1):32-9. doi: 10.1016/j.yexcr.2013.12.004
Findlay GM, Smith MJ, Lanner F, Hsiung MS, Gish GD, Petsalaki E, Cockburn K, Kaneko T, Huang H, Bagshaw RD, Ketela T, Tucholska M, Taylor L, Bowtell DD, Moffat J, Ikura M, Li SS, Sidhu SS, Rossant J, Pawson T.
Cell. 2013 Feb 28;152(5):1008-20. doi: 10.1016/j.cell.2013.01.056.
Rugg-Gunn PJ, Cox BJ, Lanner F, Sharma P, Ignatchenko V, McDonald AC, Garner J, Gramolini AO, Rossant J, Kislinger T.
Dev Cell. 2012 Apr 17;22(4):887-901. doi: 10.1016/j.devcel.2012.01.005.
Lanner F, Rossant J.
Development. 2010 Oct;137(20):3351-60. doi: 10.1242/dev.050146.
Yamanaka Y, Lanner F, Rossant J.
Development. 2010 Mar;137(5):715-24. doi: 10.1242/dev.043471.
Academic honors, awards and prizes