Resources
At iPS core facility, we use optimized protocols for culturing iPS cells, NES cells as well as differentiation towards Neurons.
Protocols from iPS Core Facility
Documents
Publication of established cell lines at iPS Core Facility:
CTRL7 iPS cells https://pubmed.ncbi.nlm.nih.gov/27789397/
CTRL9 and CTRL10 iPS cells https://pubmed.ncbi.nlm.nih.gov/28395796/
CTRL1 and CTRL3 NES cells https://pubmed.ncbi.nlm.nih.gov/28924182/
CTRL07 and CTRL10 NES cells https://pubmed.ncbi.nlm.nih.gov/31302032/
CTRL09 NES cells https://pubmed.ncbi.nlm.nih.gov/29456185/
iPS cell Generation at iPS Core Facility https://pubmed.ncbi.nlm.nih.gov/28715399/
Links to useful publications
Sai2 cells https://pubmed.ncbi.nlm.nih.gov/23884946/
AF22 NES cells https://pubmed.ncbi.nlm.nih.gov/22272239/
Dual-SMAD inhibition Protocol https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756723/
First Human iPS cell line https://pubmed-ncbi-nlm-nih-gov.proxy.kib.ki.se/18035408/
Equipment

CytoFLEX (Beckman Coulter) for FACS analysis

BioCAM DupleX (3Brain) for electrophysiological analysis

GloMax Luminescence Reader (Promega) for Mycoplasma tests