Resources

At iPS core facility, we use optimized protocols for culturing iPS cells, NES cells as well as differentiation towards Neurons.

Documents

Cultivation of hiPS cells on Laminin521 (PDF, 210.36 KB)

Freezing and thawing of hiPS cells (PDF, 144 KB)

Cultivation of human NES cells on L2020 (PDF, 169.89 KB)

Publication of established cell lines at iPS Core Facility:

CTRL7 iPS cells https://pubmed.ncbi.nlm.nih.gov/27789397/

CTRL9 and CTRL10 iPS cells https://pubmed.ncbi.nlm.nih.gov/28395796/

CTRL1 and CTRL3 NES cells https://pubmed.ncbi.nlm.nih.gov/28924182/

CTRL07 and CTRL10 NES cells https://pubmed.ncbi.nlm.nih.gov/31302032/

CTRL09 NES cells https://pubmed.ncbi.nlm.nih.gov/29456185/

iPS cell Generation at iPS Core Facility https://pubmed.ncbi.nlm.nih.gov/28715399/

Links to useful publications

Sai2 cells https://pubmed.ncbi.nlm.nih.gov/23884946/

AF22 NES cells https://pubmed.ncbi.nlm.nih.gov/22272239/

Dual-SMAD inhibition Protocol https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756723/

First Human iPS cell line https://pubmed-ncbi-nlm-nih-gov.proxy.kib.ki.se/18035408/

Equipment

iPS core instruments.

CytoFLEX (Beckman Coulter) for FACS analysis

iPS core instruments.

BioCAM DupleX (3Brain) for electrophysiological analysis

Mycoplasma

GloMax Luminescence Reader (Promega) for Mycoplasma tests