Resources

At iPS core facility, we use optimized protocols for culturing iPS cells, NES cells as well as differentiation towards Neurons.

Protocols from iPS Core Facility

 

Publication of established cell lines at iPS Core Facility:

CTRL7 iPS cells https://pubmed.ncbi.nlm.nih.gov/27789397/

CTRL9 and CTRL10 iPS cells https://pubmed.ncbi.nlm.nih.gov/28395796/

CTRL1 and CTRL3 NES cells https://pubmed.ncbi.nlm.nih.gov/28924182/

CTRL07 and CTRL10 NES cells https://pubmed.ncbi.nlm.nih.gov/31302032/

CTRL09 NES cells https://pubmed.ncbi.nlm.nih.gov/29456185/

iPS cell Generation at iPS Core Facility https://pubmed.ncbi.nlm.nih.gov/28715399/

Links to useful publications

 Sai2 cells https://pubmed.ncbi.nlm.nih.gov/23884946/

AF22 NES cells https://pubmed.ncbi.nlm.nih.gov/22272239/

Dual-SMAD inhibition Protocol https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756723/ 

First Human iPS cell line https://pubmed-ncbi-nlm-nih-gov.proxy.kib.ki.se/18035408/

Equipment 

 

 

 

 

iPS core instruments.

CytoFLEX (Beckman Coulter) for FACS analysis 

iPS core instruments.

BioCAM DupleX (3Brain) for electrophysiological analysis

Mycoplasma

GloMax Luminescence Reader (Promega) for Mycoplasma tests