Research on alternative methods
Alternative methods are being developed and tested at several universities in Sweden. The Swedish Research Council also provides funding for projects that aim to develop alternative methods that refine, reduce and/or replace animal experiments. Several research groups at Karolinska Institutet have been awarded this type of funding to use and develop alternative methods in their research.
Cultured epithelial cells in cancer research
Professor Roland Grafström uses human epithelial cells in his research. Epithelial cells coat many of the bodys surfaces, for example the skin, lungs and intestines, and Grafström was the first in Sweden to be able to culture epithelial cells without partially unknown components such as serum. This model system has given him the tools for a better understanding of how cancer develops.
Researcher Maria Isaguliants uses a method where fluorescent light enables her to monitor the immune response to an inhaled substance, so avoiding injections and other interventions.
Cultured cells to show toxicity
Professor Sandra Ceccatelli uses cultured cells to investigate toxins in the developing nervous system.
Professor Magnus Ingelman-Sundberg has developed a cell culture model to identify liver damage caused by medication. In this model, researchers use cells that have been left during liver donations.
Reprogrammed human skin cells
Researcher Anna Domogatskaya uses human skin cells that has been reprogrammed to stem cells, so-called iPS cells, to produce artificial human pancreatic islets in diabetes research.
Associate Professor Anna Falk creates cell models of the human brain, by using the iPS-technique to reprogramme skin cells to nerve cells. This method can provide vital new knowledge on difficult-to-study congenital diseases. More reading on KI News
3D model of the lung
Researcher Lena Palmberg is developing a three-dimensional model of the lung to study the effects of air pollution.