2021 Biotechnology and Bioinformatics Seminar
"Modeling cellular state and dynamics in single cell genomics"
Professor Fabian J. Theis
Institute Director, Institute of Computational Biology, Helmholtz Center Munich
Date: 25th February 2021 (Thursday)
Time: 09:30 am CET / 4:30 pm HKT
Modeling cellular state as well as dynamics e.g. during differentiation or in response to perturbations is a central goal of computational biology. Single-cell technologies now give us easy and large-scale access to state observations on the transcriptomic and more recently also epigenomic level. In particular, they allow resolving potential heterogeneities due to asynchronicity of differentiating or responding cells, and profiles across multiple conditions such as time points, space and replicates are being generated.
In this talk, Prof. Theis shortly reviewed scVelo, their recent model for dynamic RNA velocity, allowing estimation of gene-specific transcription and splicing rates, and illustrate its use to estimate a shared latent time in pancreatic endocrinogenesis. He then showed CellRank, a probabilistic model based on Markov chains which makes use of both transcriptomic similarities as well as RNA velocity to infer developmental start- and endpoints and assign lineages in a probabilistic manner. It allows users to gain insights into the timing of endocrine lineage commitment and recapitulates gene expression trends towards developmental endpoints.
While the above approaches focus on individual gene expression models, recently latent space modeling and manifold learning have become a popular tool to learn overall variation in single cell gene expression. Prof. Theis wrapped by briefly discussing how these tools can be used to integrate single cell RNA-seq data sets across multiple labs in a privacy aware manner.
Coordinator Bioinformatics Platform | Computational Biologist