H. Yesid Estupiñan Velasquez

Postdoctoral Researcher
Visiting address: Nobels väg 7, 17165 Solna
Postal address: H5 Laboratoriemedicin, H5 BCM El Andaloussi, 141 52 Huddinge

About me

  • As postdoc in Samir EL Andaloussi´s group, I am woriking on the assessment of functional macromolecular delivery enhanced by endosomolytic compounds, using in vitro reporter systems and animal models. Delivery of RNA- and protein-therapeutics targeting CNS by using bolus injection and intraventricular osmotic pumps. Evaluation of labelled-oligonucleotides and gene-editors biodistribution by measuring endogenous fluoresce using in vivo imaging systems and light sheet microscopy. Skilled in whole mouse central nervous system (CNS) micro-dissection, for the structural and functional analysis using microscopy, flow cytometry and quantitative PCR.


  • During my doctoral studies I performed phenotypic analysis of animal models used in pre-clinical studies for BTK inhibitors. 

  • In vivo and ex vivo treatments and flow cytometry analysis of B- and T-cell lineage as well as hematopoietic progenitors. In vitro evaluation of kinase inhibitors, mechanism of resistance to reversible and irreversible BTK inhibitors as when as their involvement in the BCR signally pathway. 

  • Other skills.

    Expertise in the preparation of human cadaveric specimens, dissection and morphological characterization of the vasculature system distribution pattern in CNS and heart.


  • In vivo and ex vivo assestment of small molecules used as therapeuthic alternatives or to enhance macromolecular delivery.

    Characterization of anatomical variations with clinical significance to better understand the pathophysiological processes affected by the circulatory system.


All other publications


  • Postdoctoral Researcher, Department of Laboratory Medicine, Karolinska Institutet, 2022-2024

Degrees and Education

  • Degree Of Doctor Of Philosophy, Department of Laboratory Medicine, Karolinska Institutet, 2021

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