H. Yesid Estupiñan Velasquez
About me
As postdoc in Samir EL Andaloussi´s group, I am woriking on the assessment of functional macromolecular delivery enhanced by endosomolytic compounds, using in vitro reporter systems and animal models. Delivery of RNA- and protein-therapeutics targeting CNS by using bolus injection and intraventricular osmotic pumps. Evaluation of labelled-oligonucleotides and gene-editors biodistribution by measuring endogenous fluoresce using in vivo imaging systems and light sheet microscopy. Skilled in whole mouse central nervous system (CNS) micro-dissection, for the structural and functional analysis using microscopy, flow cytometry and quantitative PCR.
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During my doctoral studies I performed phenotypic analysis of animal models used in pre-clinical studies for BTK inhibitors.
- In vivo and ex vivo treatments and flow cytometry analysis of B- and T-cell lineage as well as hematopoietic progenitors. In vitro evaluation of kinase inhibitors, mechanism of resistance to reversible and irreversible BTK inhibitors as when as their involvement in the BCR signally pathway.
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Other skills.
Expertise in the preparation of human cadaveric specimens, dissection and morphological characterization of the vasculature system distribution pattern in CNS and heart.
Research
In vivo and ex vivo assestment of small molecules used as therapeuthic alternatives or to enhance macromolecular delivery.
Characterization of anatomical variations with clinical significance to better understand the pathophysiological processes affected by the circulatory system.
Articles
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Article: BLOOD ADVANCES. 2024;8(8):1981-1990
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Journal article: INTERNATIONAL JOURNAL OF MORPHOLOGY. 2024;42(1):98-106
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Article: JOURNAL OF CLINICAL IMMUNOLOGY. 2023;43(1):136-150
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Article: FOLIA MORPHOLOGICA. 2022;81(3):559-566
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Journal article: TRANSLATIONAL RESEARCH IN ANATOMY. 2021;25:100150
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Article: RESEARCH IN VETERINARY SCIENCE. 2021;138:137-147
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Article: HEMASPHERE. 2021;5(5):e564
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Article: RESEARCH IN VETERINARY SCIENCE. 2021;135:200-216
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Journal article: NEUROLOGICAL RESEARCH. 2020;42(10):828-834
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Article: BLOOD ADVANCES. 2020;4(11):2439-2450
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Journal article: AUSTRAL JOURNAL OF VETERINARY SCIENCES. 2017;49(3):161-166
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Journal article: INTERNATIONAL JOURNAL OF MORPHOLOGY. 2017;35(1):319-324
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Journal article: FOLIA MORPHOLOGICA. 2017;76(2):262-268
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Journal article: INTERNATIONAL JOURNAL OF MORPHOLOGY. 2016;34(1):404-409
All other publications
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Thesis / dissertation: 2021
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Review: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY. 2021;9:630942
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Conference publication: BLOOD. 2019;134:5759
Employments
- Postdoctoral Researcher, Department of Laboratory Medicine, Karolinska Institutet, 2022-2024
Degrees and Education
- Degree Of Doctor Of Philosophy, Department of Laboratory Medicine, Karolinska Institutet, 2021