Yanbo Pan
Senior Research Specialist
E-mail: yanbo.pan@ki.se
Visiting address: SciLifeLab, Tomtebodavägen 23A, 17121 Solna
Postal address: K7 Onkologi-Patologi, K7 Forskning Lehtiö, 171 77 Stockholm
Research
- *Mass spectrometry (MS)-based quantitative proteomics and its clinical applications*
Proteomics is a field aiming for systems-level analysis of proteins in an organism or system. This generates new knowledge in biology to discover clinical biomarkers and drug targets. With cutting-edge MS-based quantitative proteomics (label-based and label-free), we can decipher the molecular phenotypes of different patients at the protein level. The ultimate aim is to develop differential tests to be used as clinical evaluation tools.
*Temporal spatial proteomics to improve understanding of protein level signaling in anti-cancer treatment*
Protein localization and re-localization in the different compartments of cells is an essential control mechanism for cellular functions as well as intercellular communication in multicellular organisms. we are developing methods for protein subcellular localization analysis (/Molecular Cell 2019,
Nature Reviews Methods Primers 2021, Nature Protocols 2022/) and trying to understand how proteins shuttle between different subcellular compartments in response to drug treatments and ultimately how this affects the drug sensitivity in cancer treatment.
*Functional proteomics and proteogenomics for personalized medicine*
Proteogenomics links DNA, RNA, and protein measurements into a central-dogma perspective and has the potential to improve our understanding of cancer biology. By using robust quantitative proteomics and proteogenomics to generate information about protein activity, protein complex formation, and genetic aberrant results in cancer cells and clinical tumors (/Oncogene 2019, Nature Cancer 2021/). we are generating new information to suggest novel biomarkers and drug combinations for improved cancer therapy.
Articles
- Article: ISCIENCE. 2025;28(2):111864Pan Y; Berkovska O; Marathe S; Mermelekas G; Gudoityte G; Wolide AD; Arslan T; Seashore-Ludlow B; Lehtio J; Orre LM
- Article: NATURE PROTOCOLS. 2022;17(8):1832-1867Arslan T; Pan Y; Mermelekas G; Vesterlund M; Orre LM; Lehtio J
- Article: NATURE CANCER. 2021;2(11):1224-1242Lehtio J; Arslan T; Siavelis I; Pan Y; Socciarelli F; Berkovska O; Umer HM; Mermelekas G; Pirmoradian M; Jonsson M; Brunnstrom H; Brustugun OT; Purohit KP; Cunningham R; Asl HF; Isaksson S; Arbajian E; Aine M; Karlsson A; Kotevskaz M; Hansen CG; Haakensen VD; Helland A; Tamborero D; Johansson HJ; Branca RM; Planckz M; Staaf J; Orre LM
- Article: NATURE REVIEWS METHODS PRIMERS. 2021;1(1):32Christopher JA; Stadler C; Martin CE; Morgenstern M; Pan Y; Betsinger CN; Rattray DG; Mahdessian D; Gingras A-C; Warscheid B; Lehtio J; Cristea IM; Foster LJ; Emili A; Lilley KS
- Article: ONCOGENE. 2019;38(43):6881-6897Fotouhi O; Kjellin H; Juhlin CC; Pan Y; Vesterlund M; Ghaderi M; Yousef A; Andersson-Sand H; Kharaziha P; Caramuta S; Kjellman M; Zedenius J; Larsson C; Orre LM
- Article: MOLECULAR CELL. 2019;73(1):166-182.e7Orre LM; Vesterlund M; Pan Y; Arslan T; Zhu Y; Woodbridge AF; Frings O; Fredlund E; Lehtio J
- Article: CHEMICAL COMMUNICATIONS. 2016;52(40):6689-6692Zhang Y; Pan Y; Liu W; Zhou YJ; Wang K; Wang L; Sohail M; Ye M; Zou H; Zhao ZK
- Article: ANALYTICAL CHEMISTRY. 2015;87(22):11353-11360Yao Y; Huang J; Cheng K; Pan Y; Qin H; Ye M; Zou H
- Article: PROTEOMICS. 2015;15(21):3613-3616Pan Y; Mao J; Deng Z; Dong M; Bian Y; Ye M; Zou H
- Article: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY. 2015;43(10):1452-1458Liu F-J; Ye M-L; Pan Y-B; Zou H-F
- Article: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2014;406(25):6247-6256Pan Y; Cheng K; Mao J; Liu F; Liu J; Ye M; Zou H
- Article: ANALYTICAL CHEMISTRY. 2014;86(14):6786-6791Liu F; Ye M; Pan Y; Zhang Y; Bian Y; Sun Z; Zhu J; Cheng K; Zou H
- Article: CHINESE JOURNAL OF CHROMATOGRAPHY (SE PU). 2014;32(4):376-380Zheng H; Mao J; Pan Y; Liu Z; Liu Z; Ye M; Zou H
- Article: ANALYTICAL CHEMISTRY. 2014;86(2):1170-1177Pan Y; Ye M; Zheng H; Cheng K; Sun Z; Liu F; Liu J; Wang K; Qin H; Zou H
- Article: SCIENTIFIC REPORTS. 2013;3:3460Bian Y; Ye M; Wang C; Cheng K; Song C; Dong M; Pan Y; Qin H; Zou H
- Article: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 2013;52(35):9205-9209Pan Y; Ye M; Zhao L; Cheng K; Dong M; Song C; Qin H; Wang F; Zou H
- Article: CHEMBIOCHEM. 2013;14(12):1438-1443Zhang Y; Pan Y; Yang W; Liu W; Zou H; Zhao ZK
- Article: JOURNAL OF PROTEOME RESEARCH. 2012;11(9):4673-4681Dong M; Ye M; Cheng K; Song C; Pan Y; Wang C; Bian Y; Zou H
- Article: CARBOHYDRATE RESEARCH. 2009;344(6):801-807Wang X-L; Huang Y; Zhu J; Pan Y-B; He R; Wang Y-Z
- Article: CARBOHYDRATE POLYMERS. 2008;74(4):862-867Liu G-Y; Zhai Y-L; Wang X-L; Wang W-T; Pan Y-B; Dong X-T; Wang Y-Z
All other publications
- Letter: NATURE METHODS. 2014;11(3):220-222Ye M; Pan Y; Cheng K; Zou H
Grants
- Swedish Research Council1 January 2023 - 31 December 2026RAS proteins in different locations would recruit different interaction partners and active different downstream signaling. My team will perform BioID with wide type and mutant RAS isoforms in different cancer cell lines, and then apply subcellular fractionation, farnesylation enrichment and MS-based proteomics to decipher additional functionally relevant RAS protein interactions. In addition, this research program will consider the impact of PTM and anti-RAS drug on RAS protein interaction to discover novel RAS protein interactors involved in downstream signaling or feedback and compensatory pathways which may elucidate why inhibition of downstream pathways have had limited clinical impact in cancer treatment. These data will bring novel information on poorly characterized RAS functions, e.g., its putative involvement in metabolic pathways, and on shared as well as paralog-specific protein networks that could partially explain the complexity of RAS functions. These networks of protein interactions will open numerous avenues to better understand the hidden mechanism of RAS oncogenic signaling and unveil potential therapeutic targets as well as suggest drug combinations to overcome the anti-RAS drug resistance. Moreover, the method here can be used for exploring other key proteins’ effectors to facilitate disease diagnosis and treatment.
- Prenylation proteome profiling of childhood leukemiaDr Åke Olsson Foundation1 January 2022 - 31 December 2022
- Swedish Cancer Society1 July 2018 - 30 June 2021