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About me

  • Born 1936 in Frankfurt am Main, Germany
  • Cooperation with Torbjörn Caspersson in fluorescence micro spectroscopy. MD, PhD  in 1966 at Karolinska Institutet.
  • Coooperation with Manfred Eigen, Max Planck Institute in Göttingen, Germany, and development of fluorescence relaxation for chemical kinetics.
  • Start of fluorescence fluctuation spectroscopy  now known as FCS (fluorescence correlation spectroscopy), today the method of choice for counting and analyzing single molecules.
  • 1983-1985 professor of molecular biophysics at Chemisty Centrum, University of Lund.
  • 1985-2001 professor of medical physics at Karolinska Institutet.
  • 2002-2012 research in FCS at EPFL (ETH Lausanne) in Switzerland.
  • From 2012 and ongoing at the Department of Medical Biophysics and Biochemistry, Karolinska Institutet, and at the Centre for Molecular Medicine.

Current  work

Multipoint FCS in analysis of neuro ligand-receptors in cellular networks, molecular basis of amyloid diseases together with Vladana Vukojevic and Lars Terenius.

Developoment of high precision single molecule DNA sequencing for early detection of tumor together with Gert Auer, Bo Franzen and GNOTHIS AB.

 

Links

Publications

Fluorescence-based monitoring of electronic state and ion exchange kinetics with FCS and related techniques: from T-jump measurements to fluorescence fluctuations
Rigler R, Widengren J
European biophysics journal : EBJ 2018;47(4):479-492

Time-resolved homo-FRET studies of biotin-streptavidin complexes
Andreoni A, Nardo L, Rigler R
Journal of photochemistry and photobiology. B, Biology 2016;162():656-662

Boar spermatozoa successfully predict mitochondrial modes of toxicity: implications for drug toxicity testing and the 3R principles
Vicente-carrillo A, Edebert I, Garside H, Cotgreave I, Rigler R, Loitto V, et al
Toxicology in vitro : an international journal published in association with BIBRA 2015;29(3):582-91

Probing the kinetic landscape of Hox transcription factor-DNA binding in live cells by massively parallel Fluorescence Correlation Spectroscopy
Papadopoulos Dk, Krmpot Aj, Nikolić Sn, Krautz R, Terenius L, Tomancak P, et al
Mechanisms of development 2015;138 Pt 2():218-225

A Single-Photon Avalanche Camera for Fluorescence Lifetime Imaging Microscopy and Correlation Spectroscopy
Vitali M, Bronzi D, Krmpot Aj, Nikolic Sn, Schmitt Fj, Junghans C, et al
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS 2014;20(6):-

Binding of Biotin to Streptavidin: A combined fluorescence correlation spectroscopy and time-resolved fluorescence study
Stromqvist J, Nardo L, Broekmans O, Kohn J, Lamperti M, Santamato A, et al
EUROPEAN PHYSICAL JOURNAL-SPECIAL TOPICS 2011;199(1):181-194

Fluorescence and single molecule analysis in cell biology
Rigler R
Biochemical and biophysical research communications 2010;396(1):170-5

Function and specificity of synthetic Hox transcription factors in vivo
Papadopoulos Dk, Vukojevic V, Adachi Y, Terenius L, Rigler R, Gehring Wj
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2010;107(9):4087-92

Quantitative study of synthetic Hox transcription factor-DNA interactions in live cells
Vukojevic V, Papadopoulos Dk, Terenius L, Gehring Wj, Rigler R
Proceedings of the National Academy of Sciences of the United States of America 2010;107(9):4093-8

Ethanol/naltrexone interactions at the mu-opioid receptor. CLSM/FCS study in live cells
Vukojević V, Ming Y, D'addario C, Rigler R, Johansson B, Terenius L
PloS one 2008;3(12):e4008-

Mu-opioid receptor activation in live cells
Vukojević V, Ming Y, D'addario C, Hansen M, Langel U, Schulz R, et al
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008;22(10):3537-48

Quantitative single-molecule imaging by confocal laser scanning microscopy
Vukojevic V, Heidkamp M, Ming Y, Johansson B, Terenius L, Rigler R
Proceedings of the National Academy of Sciences of the United States of America 2008;105(47):18176-81

Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy
Hassler K, Rigler P, Blom H, Rigler R, Widengren J, Lasser T
OPTICS EXPRESS 2007;15(9):5366-75

Statistical tools for the detection of memory and conformational motions in single-enzyme kinetics
Lerch Hp, Mikhailov As, Rigler R
CHEMICAL PHYSICS 2007;331(2-3):304-308

CD8 kinetically promotes ligand binding to the T-cell antigen receptor
Gakamsky Dm, Luescher If, Pramanik A, Kopito Rb, Lemonnier F, Vogel H, et al
BIOPHYSICAL JOURNAL 2005;89(3):2121-33

Fluorescence correlation spectroscopy of molecular motions and kinetics
Gösch M, Rigler R
Advanced drug delivery reviews 2005;57(1):169-90

Functional conformational motions in the turnover cycle of cholesterol oxidase
Lerch Hp, Rigler R, Mikhailov As
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2005;102(31):10807-12

Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
Gösch M, Blom H, Anderegg S, Korn K, Thyberg P, Wells M, et al
Journal of biomedical optics 2005;10(5):054008-

Personal remembrances
Fulwyler Ch, Fulwyler Cj, Fulwyler Rm, Fulwyler Jb, Arndt-jovin D, Jovin T, et al
CYTOMETRY PART A 2005;67A(2):54-60

Study of molecular events in cells by fluorescence correlation spectroscopy
Vukojević V, Pramanik A, Yakovleva T, Rigler R, Terenius L, Bakalkin G
Cellular and molecular life sciences : CMLS 2005;62(5):535-50

Direct gene expression analysis
Winter H, Korn K, Rigler R
CURRENT PHARMACEUTICAL BIOTECHNOLOGY 2004;5(2):191-7

Direct quantification of mRNA expression levels using single molecule detection
Camacho A, Korn K, Damond M, Cajot Jf, Litborn E, Liao Bh, et al
JOURNAL OF BIOTECHNOLOGY 2004;107(2):107-14

High light field confinement for fluorescent correlation spectroscopy using a solid immersion lens
Serov A, Rao R, Gosch M, Anhut T, Martin D, Brunner R, et al
BIOSENSORS & BIOELECTRONICS 2004;20(3):431-5

Parallel single molecule detection with a fully integrated single-photon 2x2 CMOS detector array
Gösch M, Serov A, Anhut T, Lasser T, Rochas A, Besse Pa, et al
Journal of biomedical optics 2004;9(5):913-21

Visualization of a functionally enhanced GFP-tagged galanin R2 receptor in PC12 cells: constitutive and ligand-induced internalization
Xia S, Kjaer S, Zheng K, Hu Ps, Bai L, Jia Jy, et al
Proceedings of the National Academy of Sciences of the United States of America 2004;101(42):15207-12

First fully integrated 2-D array of single-photon detectors in standard CMOS technology
Rochas A, Gosch M, Serov A, Besse Pa, Popovic Rs, Lasser T, et al
IEEE PHOTONICS TECHNOLOGY LETTERS 2003;15(7):963-965

Fluorescence correlation spectroscopy (FCS) analysis of human red blood cell system
Takahashi Y, Bark N, Kinjo M, Rigler R
OPTICAL REVIEW 2003;10(6):596-599

Gene expression analysis using single molecule detection
Korn K, Gardellin P, Liao B, Amacker M, Bergström A, Björkman H, et al
Nucleic acids research 2003;31(16):e89-

Suppression of exonucleolytic degradation of double-stranded DNA and inhibition of Exonuclease III by PNA
Slaitas A, Ander C, Földes-papp Z, Rigler R, Yeheskiely E
Nucleosides, nucleotides & nucleic acids 2003;22(5-8):1603-5

(13)C-(1)H NMR relaxation and fluorescence anisotropy decay study of tyrosine dynamics in motilin
Damberg P, Jarvet J, Allard P, Mets U, Rigler R, Gräslund A
Biophysical journal 2002;83(5):2812-25

Brownian motion of single molecules in electric fields
Rigler R
Electrophoresis 2002;23(5):805-8

Ligand binding of a ribosome-displayed protein detected in solution at the single molecule level by fluorescence correlation spectroscopy
Jermutus L, Kolly R, Foldes-papp Z, Hanes J, Rigler R, Pluckthun A
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 2002;31(3):179-84

Parallel flow measurements in microstructures by use of a multifocal 4 x 1 diffractive optical fan-out element
Blom H, Johansson M, Gösch M, Sigmundsson T, Holm J, Hård S, et al
Applied optics 2002;41(31):6614-20

Parallel fluorescence detection of single biomolecules in microarrays by a diffractive-optical-designed 2 x 2 fan-out element
Blom H, Johansson M, Hedman As, Lundberg L, Hanning A, Hård S, et al
Applied optics 2002;41(16):3336-42

Single-molecule surface-enhanced Raman and fluorescence correlation spectroscopy of horseradish peroxidase
Bjerneld Ej, Foldes-papp Z, Kall M, Rigler R
JOURNAL OF PHYSICAL CHEMISTRY B 2002;106(6):1213-1218

C-peptide binding to human cell membranes: importance of Glu27
Pramanik A, Ekberg K, Zhong Z, Shafqat J, Henriksson M, Jansson O, et al
Biochemical and biophysical research communications 2001;284(1):94-8

DNA-sequencing at the single molecule level
Rigler R, Seela F
JOURNAL OF BIOTECHNOLOGY 2001;86(3):161-161

Fluorescence correlation spectroscopy as a method for assessment of interactions between phage displaying antibodies and soluble antigen
Lagerkvist Ac, Földes-papp Z, Persson Ma, Rigler R
Protein science : a publication of the Protein Society 2001;10(8):1522-8

Fluorescence correlation spectroscopy detects galanin receptor diversity on insulinoma cells
Pramanik A, Olsson M, Langel U , Bartfai T, Rigler R
Biochemistry 2001;40(36):10839-45

Fluorescence correlation spectroscopy of flavins and flavoenzymes: photochemical and photophysical aspects
Van Den Berg Paw, Widengren J, Hink Ma, Rigler R, Visser Ajwg
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2001;57(11):2135-44

Fluorescent high-density labeling of DNA: error-free substitution for a normal nucleotide
Földes-papp Z, Angerer B, Ankenbauer W, Rigler R
Journal of biotechnology 2001;86(3):237-53

Fluorescently labeled model DNA sequences for exonucleolytic sequencing
Földes-papp Z, Angerer B, Thyberg P, Hinz M, Wennmalm S, Ankenbauer W, et al
Journal of biotechnology 2001;86(3):203-24

Highlight: Fluorescence correlation spectroscopy
Rigler R, Wiegrabe W
BIOLOGICAL CHEMISTRY 2001;382(3):353-354

Insulin binding monitored by fluorescence correlation spectroscopy
Zhong Zh, Pramanik A, Ekberg K, Jansson Ot, Jörnvall H, Wahren J, et al
Diabetologia 2001;44(9):1184-8

Ligand-receptor interactions in the membrane of cultured cells monitored by fluorescence correlation spectroscopy
Pramanik A, Rigler R
Biological chemistry 2001;382(3):371-8

p53 Latency. C-terminal domain prevents binding of p53 core to target but not to nonspecific DNA sequences
Yakovleva T, Pramanik A, Kawasaki T, Tan-no K, Gileva I, Lindegren H, et al
The Journal of biological chemistry 2001;276(19):15650-8

Quantitative two-color fluorescence cross-correlation spectroscopy in the analysis of polymerase chain reaction
Földes-papp Z, Rigler R
Biological chemistry 2001;382(3):473-8

Single molecule DNA sequencing in submicrometer channels: state of the art and future prospects
Sauer M, Angerer B, Ankenbauer W, Foldes-papp Z, Gobel F, Han Kt, et al
JOURNAL OF BIOTECHNOLOGY 2001;86(3):181-201

Specific binding of proinsulin C-peptide to intact and to detergent-solubilized human skin fibroblasts
Henriksson M, Pramanik A, Shafqat J, Zhong Z, Tally M, Ekberg K, et al
Biochemical and biophysical research communications 2001;280(2):423-7

The standard deviation in fluorescence correlation spectroscopy
Wohland T, Rigler R, Vogel H
BIOPHYSICAL JOURNAL 2001;80(6):2987-99

Towards a general procedure for sequencing single DNA molecules
Stephan J, Dorre K, Brakmann S, Winkler T, Wetzel T, Lapczyna M, et al
JOURNAL OF BIOTECHNOLOGY 2001;86(3):255-67

UV-Fluorescence correlation spectroscopy of 2-aminopurine
Wennmalm S, Blom H, Wallerman L, Rigler R
Biological chemistry 2001;382(3):393-7

Analysis of the RNase T1 mediated cleavage of an immobilized gapped heteroduplex via fluorescence correlation spectroscopy
Korn K, Wennmalm S, Foerster Hh, Hahn U, Rigler R
BIOLOGICAL CHEMISTRY 2000;381(3):259-63

Clustering of apoptotic cells via bystander killing by peroxides
Reznikov K, Kolesnikova L, Pramanik A, Tan-no K, Gileva I, Yakovleva T, et al
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2000;14(12):1754-64

Evidence for specific interactions between kavain and human cortical neurons monitored by fluorescence correlation spectroscopy
Boonen G, Pramanik A, Rigler R, Häberlein H
Planta medica 2000;66(1):7-10

Frontiers of skin barrier research - Biophysical perspectives on skill barrier structure and function - A Symposium in honor of Professor Bo Forslind on the occasion of his retirement - Saturday March 20, 1999, Nobel Forum, Karolinska Institutet Stockholm, Sweden - Introduction
Jornvall H, Rigler R
ACTA DERMATO-VENEREOLOGICA 2000;:4-4

Hydrodynamic flow profiling in microchannel structures by single molecule fluorescence correlation spectroscopy
Gosch M, Blom H, Holm J, Heino T, Rigler R
Analytical chemistry 2000;72(14):3260-5

Memory landscapes of single-enzyme molecules
Edman L, Rigler R
Proceedings of the National Academy of Sciences of the United States of America 2000;97(15):8266-71

Molecular interactions of peptides with phospholipid vesicle membranes as studied by fluorescence correlation spectroscopy
Pramanik A, Thyberg P, Rigler R
Chemistry and physics of lipids 2000;104(1):35-47

Role of C-peptide in human physiology
Wahren J, Ekberg K, Johansson J, Henriksson M, Pramanik A, Johansson Bl, et al
American journal of physiology. Endocrinology and metabolism 2000;278(5):E759-68

35th Annual Meeting of the European Association for the Study of Diabetes : Brussels, Belgium, 28 September-2 October 1999
Gedec Study Group, Neuropathy Study Group Of The Italian Society Of The Study Of Diabetes, European Group For The Study Of Insulin Resistance (egir), D.i.o.r. Study Group, Non-invasive Task Force, Rosiglitazone Study Group, et al
Diabetologia 1999;42(Suppl 1):A1-A330

Amyloid beta-peptide polymerization studied using fluorescence correlation spectroscopy
Tjernberg Lo, Pramanik A, Björling S, Thyberg P, Thyberg J, Nordstedt C, et al
Chemistry & biology 1999;6(1):53-62

Non-ergodic behaviour in conformational transitions of single DNA molecules
Wennmalm S, Edman L, Rigler R
CHEMICAL PHYSICS 1999;247(1):61-67

On death numbers and survival times of single dye molecules
Wennmalm S, Rigler R
JOURNAL OF PHYSICAL CHEMISTRY B 1999;103(13):2516-2519

Photodynamic properties of green fluorescent proteins investigated by fluorescence correlation spectroscopy
Widengren J, Mets U, Rigler R
CHEMICAL PHYSICS 1999;250(2):171-186

Protonation kinetics of GFP and FITC investigated by FCS - aspects of the use of fluorescent indicators for measuring pH
Widengren J, Terry B, Rigler R
CHEMICAL PHYSICS 1999;249(2-3):259-271

Resolution of fluorescence correlation measurements
Meseth U, Wohland T, Rigler R, Vogel H
BIOPHYSICAL JOURNAL 1999;76(3):1619-31

Specific binding of proinsulin C-peptide to human cell membranes
Rigler R, Pramanik A, Jonasson P, Kratz G, Jansson Ot, Nygren P, et al
Proceedings of the National Academy of Sciences of the United States of America 1999;96(23):13318-23

The fluctuating enzyme: a single molecule approach
Edman L, Foldes-papp Z, Wennmalm S, Rigler R
CHEMICAL PHYSICS 1999;247(1):11-22

The incipient stage in thrombin-induced fibrin polymerization detected by FCS at the single molecule level
Bark N, Földes-papp Z, Rigler R
Biochemical and biophysical research communications 1999;260(1):35-41

Calcium transients and the effect of a photolytically released calcium chelator during electrically induced contractions in rabbit rectococcygeus smooth muscle
Arner A, Malmqvist U, Rigler R
Biophysical journal 1998;75(4):1895-903

Fluorescence correlation spectroscopy as a tool to investigate chemical reactions in solutions and on cell surfaces
Widengren J, Rigler R
Cellular and molecular biology (Noisy-le-Grand, France) 1998;44(5):857-79

Fluorescence correlation spectroscopy of enzymatic DNA polymerization
Björling S, Kinjo M, Földes-papp Z, Hagman E, Thyberg P, Rigler R
Biochemistry 1998;37(37):12971-8

Fluorescence cross-correlation: a new concept for polymerase chain reaction
Rigler R, Földes-papp Z, Meyer-almes Fj, Sammet C, Völcker M, Schnetz A
Journal of biotechnology 1998;63(2):97-109

Heterogeneity in single DNA conformational fluctuations
Edman L, Wennmalm S, Tamsen F, Rigler R
CHEMICAL PHYSICS LETTERS 1998;292(1-2):15-21

Heterogeneity in single DNA conformational fluctuations (vol 292, pg 15, 1998)
Edman L, Wennmalm S, Tamsen F, Rigler R
CHEMICAL PHYSICS LETTERS 1998;294(6):625-625

Photobleaching of Fluorescent Dyes under Conditions Used for Single-Molecule Detection:  Evidence of Two-Step Photolysis
Eggeling C, Widengren J, Rigler R, Seidel Ca
Analytical chemistry 1998;70(13):2651-9

Single-molecule analysis of restriction DNA fragments using fluorescence correlation spectroscopy
Kinjo M, Nishimura G, Koyama T, Mets U, Rigler R
ANALYTICAL BIOCHEMISTRY 1998;260(2):166-72

A fluorescence detection and selection device for DNA-molecules, bound to microspheres
Holm J, Elderstig H, Kristensen O, Rigler R
NUCLEOSIDES & NUCLEOTIDES 1997;16(5-6):557-562

Application of the antibunching in dye fluorescence: Measuring the excitation rates in solution
Mets U, Widengren J, Rigler R
CHEMICAL PHYSICS 1997;218(1-2):191-198

Confocal spectroscopy in microstructures
Brinkmeier M, Dorre K, Riebeseel K, Rigler R
BIOPHYSICAL CHEMISTRY 1997;66(2-3):229-39

Conformational analysis of galanin using end to end distance distribution observed by Förster resonance energy transfer
Kulinski T, Wennerberg Ab, Rigler R, Provencher Sw, Pooga M, Langel U, et al
European biophysics journal : EBJ 1997;26(2):145-54

Conformational fluctuations in single DNA molecules
Wennmalm S, Edman L, Rigler R
Proceedings of the National Academy of Sciences of the United States of America 1997;94(20):10641-6

Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution
Schwille P, Meyeralmes Fj, Rigler R
BIOPHYSICAL JOURNAL 1997;72(4):1878-86

Exonuclease degradation of DNA studied by fluorescence correlation spectroscopy
Foldespapp Z, Thyberg P, Bjorling S, Holmgren A, Rigler R
NUCLEOSIDES & NUCLEOTIDES 1997;16(5-6):781-787

Fast interactions between Rh6G and dGTP in water studied by fluorescence correlation spectroscopy
Widengren J, Dapprich J, Rigler R
CHEMICAL PHYSICS 1997;216(3):417-426

Fast kinetic studies by fluorescence correlation spectroscopy of PNA-DNA interactions
Aich P, Nielsen P, Rigler R
NUCLEOSIDES & NUCLEOTIDES 1997;16(5-6):609-615

Conformational transitions monitored for single molecules in solution
Edman L, Mets U, Rigler R
Proceedings of the National Academy of Sciences of the United States of America 1996;93(13):6710-5

Fluorescence correlation spectrometry of the interaction kinetics of tetramethylrhodamin alpha-bungarotoxin with Torpedo californica acetylcholine receptor
Rauer B, Neumann E, Widengren J, Rigler R
BIOPHYSICAL CHEMISTRY 1996;58(1-2):3-12

Time-resolved fluorescence studies of the molten globule state of apomyoglobin
Rischel C, Thyberg P, Rigler R, Poulsen Fm
JOURNAL OF MOLECULAR BIOLOGY 1996;257(4):877-85

Dynamics of the peptide hormone motilin studied by time resolved fluorescence spectroscopy
Backlund Bm, Kulinski T, Rigler R, Gräslund A
European biophysics journal : EBJ 1995;23(6):407-12

FLUORESCENCE CORRELATION SPECTROSCOPY OF TRIPLET-STATES IN SOLUTION - A THEORETICAL AND EXPERIMENTAL-STUDY
Widengren J, Mets U, Rigler R
JOURNAL OF PHYSICAL CHEMISTRY 1995;99(36):13368-13379

Fluorescence correlations, single molecule detection and large number screening. Applications in biotechnology
Rigler R
Journal of biotechnology 1995;41(2-3):177-86

Ultrasensitive hybridization analysis using fluorescence correlation spectroscopy
Kinjo M, Rigler R
Nucleic acids research 1995;23(10):1795-9

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