Nutsa Burduli
Phd Student
E-mail: nutsa.burduli@ki.se
Visiting address: Alfred Nobels allé 8, plan 8, 14152 Huddinge
Postal address: H7 Medicin, Huddinge, H7 Hematologi Alici, 171 77 Stockholm
About me
- BSc (Hons) in Biochemistry from University of Galway, Ireland (2011-2015).
Degree project title: Mutation of a key enzyme residue in MSH3 subunit of MutSβ to inactivate its enzymatic function
Principal investigator: Prof. Robert Lahue
MSc in Cancer research from University of Galway, Ireland (2018-2019)
Degree project title: Development of genetically engineered Natural Killer cells for the treatment of Pancreatic Cancer
Principal investigator: Prof. Michael O'Dwyer
Immediate supervisor: Dr. Subhashis Sarkar
Currently pursuing a PhD by investigating the genetic landscape behind natural killer cell cytokine and cytotoxic responses, comparative assessment of various genetic modification strategies for NK cells and overcoming their resistance to genetic modification.
Primary investigator: Dr. Evren Alici
Co-supervisors: Assistant Prof. Arnika Kathleen Wagner, Dr. Stephan Meinke, Prof. Hans-Gustaf Ljunggren
Laboratory skills:
Multicolour flow cytometry panel design
Multicolour flow cytometry
Cell culture (primary and cell line)
Cloning
Lentiviral production
Lentiviral transduction
PBMC isolation and culture
PCR
Molecular biology
CRISPR/Cas9-mediated gene knockouts
Nucleofection/electroporation
Cytotoxicity assays
Fluorescence assisted cell sorting (FACS)
Western blotting
Gene insert design
DNA/RNA isolation and purification
Research
- NK cells
Genetic modification
Lymphocyte proliferation
CRISPR/Cas9 strategies
Lymphocyte biology
Immunotherapies
Cellular immunotherapies
Teaching
- Previous student: Fernando Herrero Quevedo
Project title: Targeted knockout of amino acid transporters ASCT1 and ASCT2 to abrogate NK cell transduction with baboon envelope pseudotyped lentiviral vectors
Current student: Kjell Klarenbeek
Project title: Evaluation of candidate genes regulating natural killer cell cytotoxic and cytokine responses
Articles
- Journal article: HELIYON. 2024;10(15):e34976
- Article: SCIENCE TRANSLATIONAL MEDICINE. 2024;16(747):eadi2952
- Article: JOURNAL OF EXPERIMENTAL AND CLINICAL CANCER RESEARCH. 2024;43(1):13
- Article: JOURNAL FOR IMMUNOTHERAPY OF CANCER. 2022;10(10):e005308
All other publications
- Meeting abstract: BLOOD. 2019;134:5536
Employments
- Phd Student, Department of Medicine, Huddinge, Karolinska Institutet, 2024-2025
Degrees and Education
- Master of Science, Cancer research, Development of genetically engineered Natural Killer cells for the treatment of Pancreatic Cancer, Medicine, National University of Ireland, Galway, 2019
- Bachelor of Science, Biochemistry, Mutation of a key enzyme residue in MSH3 subunit of MutSβ to inactivate its enzymatic function, Biological and Chemical Sciences, National University of Ireland, Galway, 2015