Denna sida på svenska
Loading profile information...

About me

I am working right now as "Senior Research Specialist" and group leader (NORDFERTIL research lab Stockholm) at the Department of Women’s and Children’s Health, Pediatric Endocrinology Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden. In addtion to my work as group leader, I am the research coordinator of NORDFERTIL (www.nordfertil.org) and dissemination manager of the EU-ITN consortium “GROWSPERM” (www.growsperm.eu).

Education

COURSES AND DEGREES

2001 - 2005 - Study of Biology, Westphalian Wilhelms University (WWU), Münster Germany

2005 - Diploma-thesis (Master Thesis; Biology), Centre of Reproductive Medicine and Andrology (CeRA), WWU, Münster, Germany & University of Pittsburgh, USA

2005 - 2009 - Ph.D.-studies (Biology), CeRA, WWU, Münster, Germany

DOCTORAL DEGREE

2009 - Biology (Reproductive Biology), Title: “In vitro differentiation of male germ cells.” CeRA, WWU, Münster, Germany; PhD supervision: Prof. Dr. E. Liebau; Prof. Dr. S. Schlatt

POSTDOC APPOINTMENTS

2009 – 2013 - Post-Doc at the Department of Women’s and Children’s Health, Pediatric Endocrinology Unit, Karolinska Institutet; supervision: Prof. Dr. Olle Söder

DOCENT-LEVEL COMPETENCE

2016 -  Docent in Reproductive Biology; Karolinska Institutet

Research description

Main research areas:

  • Germ cell differentiation in vivo and in vitro.
  • Fertility preservation techniques in children with gonadal dysfunctions.
  • Experiments on in vitro differentiation techniques include novel cell culture approaches with human and animal gonadal cells as well as human embryonic and human induced pluripotent stem cells (hESCs and hiPSCs).

Teaching portfolio

Ongoing supervision of a PhD candidate:

1) Halima Al-Balushi, Karolinska Institutet

Title: “In vitro models aiming for fertility preservation.”

Registration: 2014-04-22; halftime seminar: 2016-08-31; planned defence: 2018-11-16

main supervisor: Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden); co-supervision: Olle Söder, MD, PhD (Karolinska Institutet, Sweden); Outi Hovatta, MD, PhD (Karolinska Institutet, Sweden); Fredrik Lanner, PhD (Karolinska Institutet, Sweden)

2) Magdalena Kurek, Karolinska Institutet

Title: “Human induced pluripotent stem cells differentiation towards the male germ cell lineage.”

Registration: 2015-01-19; halftime seminar: 2017-05-31;

main supervisor: Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden); co-supervision: Olle Söder, MD, PhD (Karolinska Institutet, Sweden), Rod Mitchell, MD, PhD (University Edinburgh, UK)

3) Ieva Masliukaite, University of Amsterdam, The Netherlands

Title: “Propagation of human SSCs”.

Registration: 2015-10-20; main supervisor: Sjoerd Repping, MD, PhD (University of Amsterdam, The Netherlands), co-supervision: Ans M. M. van Pelt, PhD (University of Amsterdam, The Netherlands), Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden).

4) Melanie Poganitsch-Korhonen, University of Helsinki, Finland;

Title: “Male fertility, late morbidity and ageing after childhood cancer” Registration: 2014-09-24; main supervisor Kirsi Jahnukainen, MD, PhD (University of Helsinki, Finland), co-supervision: Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden)

5) Yanhua Cui, Karolinska Institutet

Title: “Novel strategies to preserve future fertility in boys and men.”

Registration: 2018-09-11

main supervisor: Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden); co-supervision: Kirsi Jahnukainen, MD, PhD (Karolinska Institutet, Sweden)

6) Hajar Ali Mohammed Ba Omar, Karolinska Institutet

Title: “Effects of haematological and oncological diseases on spermatogonial stem cell populations in boys.”

Registration: 2018-09-04

main supervisor: Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden); co-supervision: Kirsi Jahnukainen, MD, PhD (Karolinska Institutet, Sweden)

PhD candidates supervised up to the defence of the candidate’s doctoral thesis:

1) Ahmed Reda, Karolinska Institutet

Title: “Artificial testis to study early gonadal development and germ cell differentiation.”

Registration: 2012-04-24; halftime seminar: 2014-06-03; defence: 2016-09-30; main supervisor: Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden); co-supervision: Olle Söder, MD, PhD (Karolinska Institutet, Sweden); Kirsi Jahnukainen, MD, PhD (Karolinska Institutet, Sweden & University of Helsinki, Finland

2) Sarita Panula, Karolinska Institutet

Title: “Differentiation and charaterization of human iPSC derived germ cells and the derivation of patient specific iPSC lines for the study of infertility.” Registration: 2009-11-12; halftime seminar: 2012-09-18; defence: 2015-06-09; main supervisor: Outi Hovatta, MD, PhD (Karolinska Institutet, Sweden); co-supervision: Olle Söder, MD, PhD (Karolinska Institutet, Sweden); Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden), Renee Reijo Pera (Stanford University, USA)

3) Joao Pedro Alves Lopes, Karolinska Institutet

Title: “Novel culture systems to model testicular function and organization.” Registration: 2015-01-19; halftime seminar: 2017-06-07; defense: 2018-08-31

main supervisor: Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden); co-supervision: Olle Söder, MD, PhD (Karolinska Institutet, Sweden), Ellen Goossens, PhD (VUB, Belgium)

4) Mina Nikolova Mincheva, University of Münster, Germany

Title: “Interaction of male gonadal cell types in differentiation cell culture” Registration: 2015-01-01; defense: 2018-03-15

main supervisor: Stefan Schlatt, PhD (University of Münster, Germany), co-supervision: Sylvia Kaiser, PhD (University of Münster, Germany), Jan-Bernd Stukenborg, PhD (Karolinska Institutet, Sweden)

Links

Publications

A diagnostic germ cell score for immature testicular tissue at risk of germ cell loss
Heckmann L, Langenstroth-röwer D, Pock T, Wistuba J, Stukenborg Jb, Zitzmann M, et al
Human reproduction (Oxford, England) 2018;33(4):636-645

Cancer treatment in childhood and testicular function: the importance of the somatic environment
Stukenborg Jb, Jahnukainen K, Hutka M, Mitchell Rt
Endocrine connections 2018;7(2):R69-R87

Hormone production by human first-trimester gonads in a functional in vitro system
Albalushi H, Sahlin L, Åkesson E, Kurek M, Kjartansdóttir Kr, Lindh R, et al
Endocrinology 2018;():-

Laminin 521 Stabilizes the Pluripotency Expression Pattern of Human Embryonic Stem Cells Initially Derived on Feeder Cells
Albalushi H, Kurek M, Karlsson L, Landreh L, Kjartansdóttir Kr, Söder O, et al
Stem cells international 2018;2018():7127042-

Reassembly of adult human testicular cells: can testis cord-like structures be created in vitro?
Mincheva M, Sandhowe-klaverkamp R, Wistuba J, Redmann K, Stukenborg Jb, Kliesch S, et al
Molecular human reproduction 2018;24(2):55-63

Spermatogonial quantity in human prepubertal testicular tissue collected for fertility preservation prior to potentially sterilizing therapy
Stukenborg Jb, Alves-lopes Jp, Kurek M, Albalushi H, Reda A, Keros V, et al
Human reproduction (Oxford, England) 2018;33(9):1677-1683

Use of a three-layer gradient system of cells for rat testicular organoid generation
Alves-lopes Jp, Söder O, Stukenborg Jb
Nature protocols 2018;13(2):248-259

Decreased spermatogonial quantity in prepubertal boys with leukaemia treated with alkylating agents
Poganitsch-korhonen M, Masliukaite I, Nurmio M, Lähteenmäki P, Van Wely M, Van Pelt Amm, et al
Leukemia 2017;31(6):1460-1463

Gradient system for testicular organoids generation - a novel system to model germ to somatic cell association in vitro
Lopes Jpa, Soder O, Stukenborg Jb
HUMAN REPRODUCTION 2017;:8-8

Impact of uteroplacental insufficiency on postnatal rat male gonad
Pampanini V, Germani D, Puglianiello A, Stukenborg Jb, Reda A, Savchuk I, et al
The Journal of endocrinology 2017;232(2):247-257

In vitro re-assembly of human primary testicular cells into seminiferous cord-like structures
Mincheva M, Sandhowe-klaverkamp R, Stukenborg Jb, Wistuba J, Schlatt S
HUMAN REPRODUCTION 2017;:8-9

Knock-Out Serum Replacement and Melatonin Effects on Germ Cell Differentiation in Murine Testicular Explant Cultures
Reda A, Albalushi H, Montalvo Sc, Nurmio M, Sahin Z, Hou M, et al
Annals of biomedical engineering 2017;45(7):1783-1794

Primary Human Testicular Cells Self-Organize into Organoids with Testicular Properties
Baert Y, De Kock J, Alves-lopes Jp, Söder O, Stukenborg Jb, Goossens E
Stem cell reports 2017;8(1):30-38

Severely decreased spermatogonial quantity after chemotherapy with alkylating agents in prepubertal boys
Poganitsch-korhonen M, Masliukaite I, Nurmio M, Lahteenmaki P, Van Wely M, Van Pelt Amm, et al
HUMAN REPRODUCTION 2017;:40-41

Testicular organoid generation by a novel in vitro three-layer gradient system
Alves-lopes Jp, Söder O, Stukenborg Jb
Biomaterials 2017;130():76-89

Testicular organoids: a new model to study the testicular microenvironment in vitro?
Alves-lopes Jp, Stukenborg Jb
Human reproduction update 2017;():-

Establishing reference values for age-related spermatogonial quantity in prepubertal human testes: a systematic review and meta-analysis
Masliukaite I, Hagen Jm, Jahnukainen K, Stukenborg Jb, Repping S, Van Der Veen F, et al
Fertility and sterility 2016;106(7):1652-1657.e2

Fertility preservation in the Nordic Countries prior to HSCT in childhood or adolescence
Nordic Network Gonadal, Jahnukainen K, Ifversen M, Hasle H, Rechnitzer C, Greggains G, et al
BONE MARROW TRANSPLANTATION 2016;:S453-S453

Intrauterine Growth Restriction Affects Postnatal Testis Maturation in Rats
Pampanini V, Germani D, Puglianiello A, Stukenborg Jb, Reda A, Savchuk I, et al
HORMONE RESEARCH IN PAEDIATRICS 2016;:257-257

In vitro differentiation of rat spermatogonia into round spermatids in tissue culture
Reda A, Hou M, Winton Tr, Chapin Re, Söder O, Stukenborg Jb
Molecular human reproduction 2016;22(9):601-12

Leptin Within the Subphysiological to Physiological Range Dose Dependently Improves Male Reproductive Function in an Obesity Mouse Model
Hoffmann A, Manjowk Gm, Wagner Iv, Klöting N, Ebert T, Jessnitzer B, et al
Endocrinology 2016;157(6):2461-8

Lost in translation: The search for an in vitro screen for spermatogenic toxicity
Chapin Re, Winton T, Nowland W, Danis N, Kumpf S, Johnson K, et al
BIRTH DEFECTS RESEARCH PART B-DEVELOPMENTAL AND REPRODUCTIVE TOXICOLOGY 2016;107(6):225-242

Over Expression of NANOS3 and DAZL in Human Embryonic Stem Cells
Panula S, Reda A, Stukenborg Jb, Ramathal C, Sukhwani M, Albalushi H, et al
PloS one 2016;11(10):e0165268-

A Combination of Culture Conditions and Gene Expression Analysis Can Be Used to Investigate and Predict hES Cell Differentiation Potential towards Male Gonadal Cells
Kjartansdóttir Kr, Reda A, Panula S, Day K, Hultenby K, Söder O, et al
PloS one 2015;10(12):e0144029-

Derivation and characterization of a cytocompatible scaffold from human testis
Baert Y, Stukenborg Jb, Landreh M, De Kock J, Jörnvall H, Söder O, et al
Human reproduction (Oxford, England) 2015;30(2):256-67

Recellularization of "ghost" testicular matrix using a gas-liquid interphase method
Baert Y, Bjork L, Soder O, Stukenborg Jb, Goossens E
HUMAN REPRODUCTION 2015;:327-327

Testicular function and fertility preservation after treatment for haematological cancer
Jahnukainen K, Mitchell Rt, Stukenborg Jb
Current opinion in endocrinology, diabetes, and obesity 2015;22(3):217-23

In vitro Spermatogenesis - Optimal Culture Conditions for Testicular Cell Survival, Germ Cell Differentiation, and Steroidogenesis in Rats
Reda A, Hou M, Landreh L, Kjartansdóttir Kr, Svechnikov K, Söder O, et al
Frontiers in endocrinology 2014;5():21-

Male germ cell development in humans
Stukenborg Jb, Kjartansdóttir Kr, Reda A, Colon E, Albersmeier Jp, Söder O
Hormone research in paediatrics 2014;81(1):2-12

Mice depleted of the coxsackievirus and adenovirus receptor display normal spermatogenesis and an intact blood-testis barrier
Sultana T, Hou M, Stukenborg Jb, Töhönen V, Inzunza J, Chagin As, et al
Reproduction (Cambridge, England) 2014;147(6):875-83

Reassembly of somatic cells and testicular organogenesis in vitro
Reuter K, Ehmcke J, Stukenborg Jb, Simoni M, Damm Os, Redmann K, et al
Tissue & cell 2014;46(1):86-96

Similar causes of various reproductive disorders in early life
Svechnikov K, Stukenborg Jb, Savchuck I, Söder O
Asian journal of andrology 2014;16(1):50-9

The gut microbiota and developmental programming of the testis in mice
Al-asmakh M, Stukenborg Jb, Reda A, Anuar F, Strand Ml, Hedin L, et al
PloS one 2014;9(8):e103809-

Phenotype and steroidogenic potential of PDGFRα-positive rat neonatal peritubular cells
Landreh L, Stukenborg Jb, Söder O, Svechnikov K
Molecular and cellular endocrinology 2013;372(1-2):96-104

Boys with Undescended Testes: Endocrine, Volumetric and Morphometric Studies on Testicular Function before and after Orchidopexy at Nine Months or Three Years of Age
Kollin C, Stukenborg Jb, Nurmio M, Sundqvist E, Gustafsson T, Soder O, et al
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM 2012;97(12):4588-95

Differentiation of stem cells upon deprivation of exogenous FGF2: a general approach to study spontaneous differentiation of hESCs in vitro
Kjartansdottir Kr, Gabrielsen A, Reda A, Soder O, Bergstrom-tengzelius R, Andersen Cy, et al
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE 2012;58(6):330-8

Insulin solubility transitions by pH-dependent interactions with proinsulin C-peptide
Landreh M, Alvelius G, Willander H, Stukenborg Jb, Soder O, Johansson J, et al
FEBS JOURNAL 2012;279(24):4589-97

Minutes of the 5th meeting of the International Network for Young Researchers in Male Fertility
Amaral A, Wahlgren A, Tüttelmann F, De Gendt K, Blomberg Jensen M, Nurmio M, et al
Asian journal of andrology 2012;14(5):796-

Present and Future Prospects of Male Fertility Preservation for Children and Adolescents
Jahnukainen K, Stukenborg Jb
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM 2012;97(12):4341-51

Proinsulin C-peptide interferes with insulin fibril formation
Landreh M, Stukenborg Jb, Willander H, Soder O, Johansson J, Jornvall H
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2012;418(3):489-93

The future of testis research is turning 6! Six years of International Network for Young Researchers in Male Fertility
Tuttelmann F, De Gendt K, Amaral A, Giachini Cc, Welsh M, Jensen Mb, et al
INTERNATIONAL JOURNAL OF ANDROLOGY 2012;35(2):211-3

Ontogenesis of Ap-2 gamma Expression in Rat Testes
Hou M, Stukenborg Jb, Nurmio M, Andersson M, Toppari J, Soder O, et al
SEXUAL DEVELOPMENT 2011;5(4):188-96

3D-culture systems to define early germ cell differentiation in vitro
Stukenborg Jb, Landreh L, Hou M, Soder O
INTERNATIONAL JOURNAL OF ANDROLOGY 2010;:81-81

Male 41, XXY* Mice as a Model for Klinefelter Syndrome: Hyperactivation of Leydig Cells
Wistuba J, Luetjens Cm, Stukenborg Jb, Poplinski A, Werler S, Dittmann M, et al
ENDOCRINOLOGY 2010;151(6):2898-910

Ontogenesis of Testis Development and Function in Humans
Stukenborg Jb, Colon E, Soder O
SEXUAL DEVELOPMENT 2010;4(4-5):199-212

In Vitro Generation of Mouse Sperm Using 3D-Culture Systems
Schlatt S, Stukenborg Jb, Huleihel M, Wistuba J
BIOLOGY OF REPRODUCTION 2009;:89-90

New horizons for in vitro spermatogenesis? An update on novel three-dimensional culture systems as tools for meiotic and post-meiotic differentiation of testicular germ cells
Stukenborg Jb, Schlatt S, Simoni M, Yeung Ch, Abu Elhija M, Luetjens Cm, et al
MOLECULAR HUMAN REPRODUCTION 2009;15(9):521-9

Coculture of spermatogonia with somatic cells in a novel three-dimensional soft-agar-culture-system
Stukenborg Jb, Wistuba J, Luetjens Cm, Abu Elhija M, Huleihel M, Lunenfeld E, et al
JOURNAL OF ANDROLOGY 2008;29(3):312-29

Complete spermatogenesis in orthotopic but not in ectopic transplants of autologously grafted marmoset testicular tissue
Luetjens Cm, Stukenborg Jb, Nieschlag E, Simoni M, Wistuba J
ENDOCRINOLOGY 2008;149(4):1736-47

Minutes of the 14th European workshop on molecular and cellular endocrinology of the testis
Gromoll J, Michel C, Kostova E, Tüttelmann F, Wistuba J, Yeung Ch, et al
Molecular and cellular endocrinology 2006;257-258():1-5

Iporin, a direct interacting partner of Rab 1b plays a role during spermatogenesis
Konczal M, Gromoll J, Von Schonfeldt V, Stukenborg Jb, Barnekow A
EUROPEAN JOURNAL OF CELL BIOLOGY 2005;:71-71

Show all publications