Axel Leppert

Publications
CV
Public outreach and news

Articles

Article: ADVANCED FUNCTIONAL MATERIALS. 2024;34(23)
Spiders Use Structural Conversion of Globular Amyloidogenic Domains to Make Strong Silk Fibers
Qi X; Wang H; Wang K; Wang Y; Leppert A; Iashchishyn I; Zhong X; Zhou Y; Liu R; Rising A; Landreh M; Johansson J; Chen G
Journal article: BIOPHYSICAL JOURNAL. 2024;123(3):413a-414a
The anti-amyloid BRICHOS domain regulates tau protein liquid-liquid phase separation and inhibits tau fibrillation associated with Alzheimer's disease
Morman C; Leppert A; Johansson J; Luo J; Abelein A
Article: NATURE PROTOCOLS. 2023;18(12):3653-3661
Advances in mass spectrometry to unravel the structure and function of protein condensates
Sahin C; Leppert A; Landreh M
Article: NATURE COMMUNICATIONS. 2023;14(1):7636
The heat shock protein LarA activates the Lon protease in response to proteotoxic stress
Omnus DJ; Fink MJ; Kallazhi A; Xandri Zaragoza M; Leppert A; Landreh M; Jonas K
Article: SMALL. 2023;19(46)
Spider Silk Protein Forms Amyloid-Like Nanofibrils through a Non-Nucleation-Dependent Polymerization Mechanism (Small 46/2023)
Qi X; Wang Y; Yu H; Liu R; Leppert A; Zheng Z; Zhong X; Jin Z; Wang H; Li X; Wang X; Landreh M; Morozova-Roche LA; Johansson J; Xiong S; Iashchishyn I; Chen G
Article: SMALL. 2023;19(46):e2304031
Spider Silk Protein Forms Amyloid-Like Nanofibrils through a Non-Nucleation-Dependent Polymerization Mechanism
Qi X; Wang Y; Yu H; Liu R; Leppert A; Zheng Z; Zhong X; Jin Z; Wang H; Li X; Wang X; Landreh M; Morozova-Roche LA; Johansson J; Xiong S; Iashchishyn I; Chen G
Article: ANALYTICAL CHEMISTRY. 2023;95(29):10869-10872
Monitoring Disassembly and Cargo Release of Phase-Separated Peptide Coacervates with Native Mass Spectrometry
Cerrato CPP; Leppert A; Sun Y; Lane DPP; Arsenian-Henriksson M; Miserez A; Landreh M
Article: COMMUNICATIONS BIOLOGY. 2023;6(1):497
Short hydrophobic loop motifs in BRICHOS domains determine chaperone activity against amorphous protein aggregation but not against amyloid formation
Chen G; Leppert A; Poska H; Nilsson HE; Alvira CP; Zhong X; Koeck P; Jegerschold C; Abelein A; Hebert H; Johansson J
Article: PNAS NEXUS. 2023;2(2):pgac303
A "grappling hook" interaction connects self-assembly and chaperone activity of Nucleophosmin 1
Saluri M; Leppert A; Gese GV; Sahin C; Lama D; Kaldmae M; Chen G; Elofsson A; Allison TM; Arsenian-Henriksson M; Johansson J; Lane DP; Hallberg BM; Landreh M
Article: RSC CHEMICAL BIOLOGY. 2022;3(11):1342-1358
Abilities of the BRICHOS domain to prevent neurotoxicity and fibril formation are dependent on a highly conserved Asp residue
Chen G; Andrade-Talavera Y; Zhong X; Hassan S; Biverstal H; Poska H; Abelein A; Leppert A; Kronqvist N; Rising A; Hebert H; Koeck PJB; Fisahn A; Johansson J
Article: MOLECULAR & CELLULAR PROTEOMICS. 2022;21(10):100413
Mass Spectrometry and Machine Learning Reveal Determinants of Client Recognition by Antiamyloid Chaperones
Osterlund N; Vosselman T; Leppert A; Graslund A; Jornvall H; Ilag LL; Marklund EG; Elofsson A; Johansson J; Sahin C; Landreh M
Article: PROTEIN SCIENCE. 2022;31(8):e4378
ATP-independent molecular chaperone activity generated under reducing conditions
Leppert A; Chen G; Lianoudaki D; Williams C; Zhong X; Gilthorpe JD; Landreh M; Johansson J
Article: JACS AU. 2021;1(12):2385-2393
Charge Engineering Reveals the Roles of Ionizable Side Chains in Electrospray Ionization Mass Spectrometry
Abramsson ML; Sahin C; Hopper JTS; Branca RMM; Danielsson J; Xu M; Chandler SA; Osterlund N; Ilag LL; Leppert A; Costeira-Paulo J; Lang L; Teilum K; Laganowsky A; Benesch JLP; Oliveberg M; Robinson CV; Marklund EG; Allison TM; Winther JR; Landreh M
Article: BIOCHEMISTRY. 2021;60(9):678-688
Smallest Secondary Nucleation Competent Aβ Aggregates Probed by an ATP-Independent Molecular Chaperone Domain
Leppert A; Tiiman A; Kronqvist N; Landreh M; Abelein A; Vukojevic V; Johansson J
Article: CHEMICAL COMMUNICATIONS. 2021;57(12):1450-1453
Ion mobility-mass spectrometry shows stepwise protein unfolding under alkaline conditions
Sahin C; Osterlund N; Leppert A; Johansson J; Marklund EG; Benesch JLP; Ilag LL; Allison TM; Landreh M
Article: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2021;25(1):434-447
Maresin 1 attenuates pro-inflammatory activation induced by β-amyloid and stimulates its uptake
Wang Y; Leppert A; Tan S; van der Gaag B; Li N; Schultzberg M; Hjorth E
Article: THE FEBS JOURNAL. 2020;287(13):2823-2833
High intracellular stability of the spidroin N-terminal domain in spite of abundant amyloidogenic segments revealed by in-cell hydrogen/deuterium exchange mass spectrometry
Kaldmae M; Leppert A; Chen G; Sarr M; Sahin C; Nordling K; Kronqvist N; Gonzalvo-Ulla M; Fritz N; Abelein A; Lain S; Biverstal H; Jornvall H; Lane DP; Rising A; Johansson J; Landreh M
Article: SCIENTIFIC REPORTS. 2020;10(1):9817
Recombinant Bri3 BRICHOS domain is a molecular chaperone with effect against amyloid formation and non-fibrillar protein aggregation
Poska H; Leppert A; Tigro H; Zhong X; Kaldmae M; Nilsson HE; Hebert H; Chen G; Johansson J
Article: MOLECULAR AND CELLULAR NEUROSCIENCE. 2020;105:103498
Recombinant BRICHOS chaperone domains delivered to mouse brain parenchyma by focused ultrasound and microbubbles are internalized by hippocampal and cortical neurons
Galan-Acosta L; Sierra C; Leppert A; Pouliopoulos AN; Kwon N; Noel RL; Tambaro S; Presto J; Nilsson P; Konofagou EE; Johansson J
Article: COMMUNICATIONS BIOLOGY. 2020;3(1):32
Augmentation of Bri2 molecular chaperone activity against amyloid-β reduces neurotoxicity in mouse hippocampus in vitro
Chen G; Andrade-Talavera Y; Tambaro S; Leppert A; Nilsson HE; Zhong X; Landreh M; Nilsson P; Hebert H; Biverstal H; Fisahn A; Abelein A; Johansson J
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2019;294(8):2606-2615
Blood-brain and blood-cerebrospinal fluid passage of BRICHOS domains from two molecular chaperones in mice
Tambaro S; Galan-Acosta L; Leppert A; Chen G; Biverstal H; Presto J; Nilsson P; Johansson J
Journal article: ALZHEIMER'S AND DEMENTIA. 2018;14(7S_Part_13):p727
P2‐155: THIOREDOXIN 80 MAINTAINS INSULIN ACTIVITY BY PREVENTING ITS AGGREGATION: IMPLICATIONS FOR ALZHEIMER'S DISEASE
Winblad B; Rodriguez PR; Garrido JC; Leppert A; Persson T; Acosta LG; Minguez AC
Article: NATURE COMMUNICATIONS. 2017;8(1):2081
Bri2 BRICHOS client specificity and chaperone activity are governed by assembly state
Chen G; Abelein A; Nilsson HE; Leppert A; Andrade-Talavera Y; Tambaro S; Hemmingsson L; Roshan F; Landreh M; Biverstal H; Koeck PJB; Presto J; Hebert H; Fisahn A; Johansson J
Article: BIOCHIMICA ET BIOPHYSICA ACTA: INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND BIOPHYSICS. 2015;1854(8):835-843
Dissociation of a BRICHOS trimer into monomers leads to increased inhibitory effect on Aβ42 fibril formation
Biverstal H; Dolfe L; Hermansson E; Leppert A; Reifenrath M; Winblad B; Presto J; Johansson J
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Preprint: BIORXIV. 2024
Controlling Drug Partitioning in Individual Protein Condensates through Laser-Induced Microscale Phase Transitions
Leppert A; Feng J; Railaite V; Bohn Pessatti T; Mörman C; Os-terholz H; Maia FRNC; Linder M; Rising A; Landreh M
Letter: NANO LETTERS. 2023;23(12):5836-5841
Liquid-Liquid Phase Separation Primes Spider Silk Proteins for Fiber Formation via a Conditional Sticker Domain
Leppert A; Chen G; Lama D; Sahin C; Railaite V; Shilkova O; Arndt T; Marklund EG; Lane DP; Rising A; Landreh M
Review: PROTEIN SCIENCE. 2023;32(6):e4645
A new kid in the folding funnel: Molecular chaperone activities of the BRICHOS domain
Leppert A; Poska H; Landreh M; Abelein A; Chen G; Johansson J
Conference publication: EUROPEAN BIOPHYSICS JOURNAL. 2023;52(SUPPL 1):S97
The anti-amyloid chaperone BRICHOS regulates Tau liquid-liquid phase separation and inhibits Tau fibrillation
Morman C; Leppert A; Johansson J; Luo J; Abelein A
Preprint: BIORXIV. 2022
A “grappling hook” interaction balances self-assembly and chaperone activity of Nucleophosmin 1
Saluri M; Leppert A; Gese GV; Sahin C; Lama D; Kaldmäe M; Chen G; Elofsson A; Allison T; Arsenian-Henriksson M; Johansson J; Lane D; Hällberg M; Landreh M
Preprint: BIORXIV. 2021
Molecular chaperone ability to inhibit amyloid-derived neurotoxicity, but not amorphous protein aggregation, depends on a conserved pH-sensitive Asp residue
Chen G; Andrade-Talavera Y; Zhong X; Hassan S; Biverstal H; Poska H; Abelein A; Leppert A; Kronqvist N; Rising A; Hebert H; Koeck PJB; Fisahn A; Johansson J
Thesis / dissertation: 2020
Molecular basis for chaperone activities of the BRICHOS domain against different types of clumpy clients : a route to prevent amyloid toxicity
Leppert, Axel
Meeting abstract: AMYLOID : THE INTERNATIONAL JOURNAL OF EXPERIMENTAL AND CLINICAL INVESTIGATION : THE OFFICIAL JOURNAL OF THE INTERNATIONAL SOCIETY OF AMYLOIDOSIS. 2019;26(sup1):152-153
BRICHOS: a chaperone with different activities depending on quaternary structure and cellular location?
Leppert A; Chen G; Johansson J
Meeting abstract: AMYLOID : THE INTERNATIONAL JOURNAL OF EXPERIMENTAL AND CLINICAL INVESTIGATION : THE OFFICIAL JOURNAL OF THE INTERNATIONAL SOCIETY OF AMYLOIDOSIS. 2017;24(sup1):7-8
BRICHOS - an anti-amyloid chaperone: evaluation of blood-brain barrier permeability of Bri2 BRICHOS
Tambaro S; Galan-Acosta L; Leppert A; Presto J; Johansson J
Conference publication: PROTEIN SCIENCE. 2017;26:31-32
Bri2 BRICHOS molecular chaperone activity is decoupled from its ability to inhibit amyloid fibril formation
Chen G; Abelein A; Leppert A; Tambaro S; Biverstal H; Presto J; Johansson J

Employments

Affiliated to Research, Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 2024-2026

Degrees and Education

Degree Of Doctor Of Philosophy, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, 2020

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