Wound age estimation

One of the most common and important questions in forensic death investigations is the time when injuries have been sustained. A deceased subject, either a victim of homicide, suicide or a fatal accident, may have died immediately, or several minutes, hours or even a day, after the injuries were sustained. We evaluating certain immunohistochemistry markers in terms of their capacity to predict the post-infliction time interval. We are also taking advantage of the proximity ligation assay technique, using combination of antibodies to proteins that are assumed to interact in different time phases after a vessel injury. Further, RNA expression will be analyzed in samples from the same wounds. Different non-invasive sample techniques that may allow for analysis of wounds in living subjects will also be evaluated. Further, methodological work in under way to develop a protocol for analysis of protein interactions in extracts from tissue samples