Birgitta Henriques-Normark Group


Birgitta Henriques-Normark is Professor in medical microbial pathogenesis at MTC as well as a Medical Doctor (MD) with speciality in clinical bacteriology.

The main research interest is host-bacterial interactions. This spans many projects, ranging from bacterial regulatory systems to the immune response of the host. Much work is done together with other groups in a larger constellation of researchers to facilitate introduction of new techniques and ideas.

Project Groups Within The Birgitta Henriques-Normark Group

Jan-Ingmar Flock

Edmund Loh


Host-pathogen interactions in health and disease

Our research focuses mainly on host-bacterial interactions, with projects ranging from bacterial regulatory systems to innate immune responses in the host. The main research program includes studies on the molecular epidemiology and pathogenesis of pneumococcal infections, from the clinical side to more basic approaches looking at host-pathogen interactions. Also, antibiotic resistance development is targeted.

Streptococcus pneumoniae is a common cause of morbidity and mortality worldwide, killing 1 to 2 million people every year. Despite being devastating pathogens they are also frequent colonizers of the upper respiratory tract of healthy children attending day-care centers. We have studied the molecular epidemiology of invasive pneumococcal disease and found that certain serotypes and clones are more prone to give raise to invasive disease, such as clones of type 1 and 7F. These types are more commonly found among previously healthy individuals. We have also identified novel virulence factors such as a pilus like structure important for adherence, colonization, and virulence, as well as for the inflammatory response. Furthermore we have shown that pneumococci are trapped but not killed by so called neutrophil extracellular traps (NETs). However, the bacteria may free themselves from NETs by using an endonuclease, thereby promoting spread to the lungs and the bloodstream. Recently we have found that of the Toll-like receptors (TLR) 1,2,4,6 and 9, only TLR 9 plays a non-redundant role in pneumococcal infections. Other research includes studies of inhibitors of the type III secretion system in Chlamydia trachomatis, an enzymatic ruler that modulates Lewis antigen glycosylation of Helicobacter pylori lipopolysaccharide during persistent infection, antimicrobial peptides, and invasive Group A streptococcal infections. We will also continue our work on the recently described pilus-like structure and other potential vaccine candidates, as well as on type III secretion inhibitors for drug development.

The work can tentatively be divided into the following areas:

1. The innate immune defense and bacterial infections.
The interaction between the innate immune system and microbial pathogens including for example Toll like receptors, NODs, scavenger receptors and antimicrobial peptides.

2. Streptococcus pneumoniae (pneumococci).
Involves many aspects of this pathogen, ranging from its epidemiology and antibiotic resistance to virulence and pathogenesis aspects and the interaction with the innate immune system.

3. Helicobacter
To understand the interactions with the host and why some strains are more virulent than others.

4. Regulation of virulence genes.
Escherichia coli and Salmonella are the main models to look at gene regulatory mechanisms involved in virulence and adaptation to new environments.

5. Bacterial adhesion organelles.
Studying the biogenesis of adhesion organelles, such as pili and curli. But also their role in disease.

6. Cell wall synthesis.
Includes projects from reageneration of cell wall components and programmed cell death in bacteria to the function and regulation of beta-lactamases.

7. Chlamydia species.
Specifically we are studying inhibitors of the type III secretion system.
To find out more details please have a look at our recent publications.

Research Constellation Host-microbe interactions Publications

Research Constellation Host-microbe interactions Publications (pdf file)


Publications - Birgitta Henriques-Normark

The Role of Microglia in Bacterial Meningitis: Inflammatory Response, Experimental Models and New Neuroprotective Therapeutic Strategies.
Thorsdottir S, Henriques-Normark B, Iovino F
Front Microbiol 2019 ;10():576

IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo.
Sierakowiak A, Henriques-Normark B, Iovino F
Methods Mol. Biol. 2019 ;1968():195-202

In Vivo Mouse Models to Study Pneumococcal Host Interaction and Invasive Pneumococcal Disease.
Iovino F, Sender V, Henriques-Normark B
Methods Mol. Biol. 2019 ;1968():173-181

High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host.
Iovino F, Henriques-Normark B
Methods Mol. Biol. 2019 ;1968():53-59

Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell.
Iovino F, Henriques-Normark B
Methods Mol. Biol. 2019 ;1968():35-39

Luminescent CeO:Eu nanocrystals for robust in situ HO real-time detection in bacterial cell cultures.
Henning D, Merkl P, Yun C, Iovino F, Xie L, Mouzourakis E, et al
Biosens Bioelectron 2019 May;132():286-293

Gram-Positive Type IV Pili and Competence.
Muschiol S, Aschtgen M, Nannapaneni P, Henriques-Normark B
Microbiol Spectr 2019 01;7(1):

Pathogenesis and prevention of risk of cardiovascular events in patients with pneumococcal community-acquired pneumonia.
Feldman C, Normark S, Henriques-Normark B, Anderson R
J. Intern. Med. 2019 Jun;285(6):635-652

Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival.
Subramanian K, Neill D, Malak H, Spelmink L, Khandaker S, Dalla Libera Marchiori G, et al
Nat Microbiol 2019 01;4(1):62-70

Eosinophilia and reduced STAT3 signaling affect neutrophil cell death in autosomal-dominant Hyper-IgE syndrome.
Farmand S, Kremer B, Häffner M, Pütsep K, Bergman P, Sundin M, et al
Eur. J. Immunol. 2018 12;48(12):1975-1988

Aetiology of lower respiratory tract infection in adults in primary care: a prospective study in 11 European countries.
Ieven M, Coenen S, Loens K, Lammens C, Coenjaerts F, Vanderstraeten A, et al
Clin. Microbiol. Infect. 2018 Nov;24(11):1158-1163

Factor H binding proteins protect division septa on encapsulated Streptococcus pneumoniae against complement C3b deposition and amplification.
Pathak A, Bergstrand J, Sender V, Spelmink L, Aschtgen M, Muschiol S, et al
Nat Commun 2018 08;9(1):3398

Clinical relevance of bacterial resistance in lower respiratory tract infection in primary care: secondary analysis of a multicentre European trial.
Teepe J, Broekhuizen B, Goossens H, Hordijk P, Loens K, Lammens C, et al
Br J Gen Pract 2018 Sep;68(674):e627-e632

Receptor Blockade: A Novel Approach to Protect the Brain From Pneumococcal Invasion.
Iovino F, Thorsdottir S, Henriques-Normark B
J. Infect. Dis. 2018 Jul;218(3):476-484

Immunomodulatory Effects of Pneumococcal Extracellular Vesicles on Cellular and Humoral Host Defenses.
Codemo M, Muschiol S, Iovino F, Nannapaneni P, Plant L, Wai S, et al
MBio 2018 04;9(2):

Aetiology of lower respiratory tract infection in adults in primary care: a prospective study in 11 European countries.
Ieven M, Coenen S, Loens K, Lammens C, Coenjaerts F, Vanderstraeten A, et al
Clin. Microbiol. Infect. 2018 Nov;24(11):1158-1163

Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood.
Toepfner N, Herold C, Otto O, Rosendahl P, Jacobi A, Kräter M, et al
Elife 2018 01;7():

Separation of pathogenic bacteria by chain length.
Beech J, Ho B, Garriss G, Oliveira V, Henriques-Normark B, Tegenfeldt J
Anal. Chim. Acta 2018 Feb;1000():223-231

Comparison of the Impact of Pneumococcal Conjugate Vaccine 10 or Pneumococcal Conjugate Vaccine 13 on Invasive Pneumococcal Disease in Equivalent Populations.
Naucler P, Galanis I, Morfeldt E, Darenberg J, Örtqvist , Henriques-Normark B
Clin. Infect. Dis. 2017 11;65(11):1780-1789

Effect of high-valency pneumococcal conjugate vaccines on invasive pneumococcal disease in children in SpIDnet countries: an observational multicentre study.
Savulescu C, Krizova P, Lepoutre A, Mereckiene J, Vestrheim D, Ciruela P, et al
Lancet Respir Med 2017 08;5(8):648-656

Analysis of IAV Replication and Co-infection Dynamics by a Versatile RNA Viral Genome Labeling Method.
Dou D, Hernández-Neuta I, Wang H, Östbye H, Qian X, Thiele S, et al
Cell Rep 2017 07;20(1):251-263

Structure of the competence pilus major pilin ComGC in Streptococcus pneumoniae.
Muschiol S, Erlendsson S, Aschtgen M, Oliveira V, Schmieder P, de Lichtenberg C, et al
J. Biol. Chem. 2017 08;292(34):14134-14146

pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion
Federico Iovino, Joo-Yeon Engelen-Lee, Matthijs Brouwer, Diederik van de Beek, Arie van der Ende, Merche Valls Seron, Peter Mellroth, Sandra Muschiol, Jan Bergstrand, Jerker Widengren, View ORCID ProfileBirgitta Henriques-Normark
JEM  DOI: 10.1084/jem.20161668 | Published May 17, 2017

Widespread and Indiscriminate Nanosilver Use: Genuine Potential for Microbial Resistance.
Gunawan C, Marquis C, Amal R, Sotiriou G, Rice S, Harry E
ACS Nano 2017 04;11(4):3438-3445

The Pneumocell-study: Vaccination of IgG1- and IgG2-deficient patients with Prevnar13.
Zangenah S, Björkhem-Bergman L, Norlin A, Hansen S, Lindqvist L, Henriques-Normark B, et al
Vaccine 2017 05;35(20):2654-2660

Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils.
Subramanian K, Bergman P, Henriques-Normark B
J Innate Immun 2017 ;9(4):375-386

Coeliac disease and invasive pneumococcal disease: a population-based cohort study.
Röckert Tjernberg A, Bonnedahl J, Inghammar M, Egesten A, Kahlmeter G, Nauclér P, et al
Epidemiol. Infect. 2017 04;145(6):1203-1209

N-acetylglucosamine-Mediated Expression of nagA and nagB in Streptococcus pneumoniae.
Afzal M, Shafeeq S, Manzoor I, Henriques-Normark B, Kuipers O
Front Cell Infect Microbiol 2016 ;6():158

Pneumococcal Carriage in Children under Five Years in Uganda-Will Present Pneumococcal Conjugate Vaccines Be Appropriate?
Lindstrand A, Kalyango J, Alfvén T, Darenberg J, Kadobera D, Bwanga F, et al
PLoS ONE 2016 ;11(11):e0166018

Impact of AmpC Derepression on Fitness and Virulence: the Mechanism or the Pathway?
Pérez-Gallego M, Torrens G, Castillo-Vera J, Moya B, Zamorano L, Cabot G, et al
MBio 2016 10;7(5):

Influenza A Virus Infection Predisposes Hosts to Secondary Infection with Different Streptococcus pneumoniae Serotypes with Similar Outcome but Serotype-Specific Manifestation.
Sharma-Chawla N, Sender V, Kershaw O, Gruber A, Volckmar J, Henriques-Normark B, et al
Infect. Immun. 2016 Dec;84(12):3445-3457

Streptococcus pneumoniae Senses a Human-like Sialic Acid Profile via the Response Regulator CiaR.
Hentrich K, Löfling J, Pathak A, Nizet V, Varki A, Henriques-Normark B
Cell Host Microbe 2016 Sep;20(3):307-317

Cinobufagin Modulates Human Innate Immune Responses and Triggers Antibacterial Activity.
Xie S, Spelmink L, Codemo M, Subramanian K, Pütsep K, Henriques-Normark B, et al
PLoS ONE 2016 ;11(8):e0160734

Unaltered pneumococcal carriage prevalence due to expansion of non-vaccine types of low invasive potential 8years after vaccine introduction in Stockholm, Sweden.
Lindstrand A, Galanis I, Darenberg J, Morfeldt E, Naucler P, Blennow M, et al
Vaccine 2016 08;34(38):4565-4571

Pneumococcal meningitis is promoted by single cocci expressing pilus adhesin RrgA.
Iovino F, Hammarlöf D, Garriss G, Brovall S, Nannapaneni P, Henriques-Normark B
J. Clin. Invest. 2016 08;126(8):2821-6

The crystal structure of the major pneumococcal autolysin LytA in complex with a large peptidoglycan fragment reveals the pivotal role of glycans for lytic activity.
Sandalova T, Lee M, Henriques-Normark B, Hesek D, Mobashery S, Mellroth P, et al
Mol. Microbiol. 2016 09;101(6):954-67

lytA-based identification methods can misidentify Streptococcus pneumoniae.
Simões A, Tavares D, Rolo D, Ardanuy C, Goossens H, Henriques-Normark B, et al
Diagn. Microbiol. Infect. Dis. 2016 Jun;85(2):141-8

Toll-Like Receptor 3/TRIF-Dependent IL-12p70 Secretion Mediated by Streptococcus pneumoniae RNA and Its Priming by Influenza A Virus Coinfection in Human Dendritic Cells.
Spelmink L, Sender V, Hentrich K, Kuri T, Plant L, Henriques-Normark B
MBio 2016 Mar;7(2):e00168-16

How Does Streptococcus pneumoniae Invade the Brain?
Iovino F, Seinen J, Henriques-Normark B, van Dijl J
Trends Microbiol. 2016 Apr;24(4):307-315

Effects of PCV7 and PCV13 on invasive pneumococcal disease and carriage in Stockholm, Sweden.
Galanis I, Lindstrand A, Darenberg J, Browall S, Nannapaneni P, Sjöström K, et al
Eur. Respir. J. 2016 Apr;47(4):1208-18

Variation in Inflammatory Response during Pneumococcal Infection Is Influenced by Host-Pathogen Interactions but Associated with Animal Survival.
Jonczyk M, Escudero L, Sylvius N, Norman M, Henriques-Normark B, Andrew P
Infect. Immun. 2016 Apr;84(4):894-905

Lung epithelium and myeloid cells cooperate to clear acute pneumococcal infection.
Dudek M, Puttur F, Arnold-Schrauf C, Kühl A, Holzmann B, Henriques-Normark B, et al
Mucosal Immunol 2016 09;9(5):1288-302

Streptococcal M1 Strikes by Neutralizing Cathelicidins.
Henriques-Normark B, Normark S
Cell Host Microbe 2015 Oct;18(4):390-1

BCG Skin Infection Triggers IL-1R-MyD88-Dependent Migration of EpCAMlow CD11bhigh Skin Dendritic cells to Draining Lymph Node During CD4+ T-Cell Priming.
Bollampalli V, Harumi Yamashiro L, Feng X, Bierschenk D, Gao Y, Blom H, et al
PLoS Pathog. 2015 Oct;11(10):e1005206

Respiratory viruses associated with community-acquired pneumonia in children: matched case-control study.
Rhedin S, Lindstrand A, Hjelmgren A, Ryd-Rinder M, Öhrmalm L, Tolfvenstam T, et al
Thorax 2015 Sep;70(9):847-53

Clinical manifestations of invasive pneumococcal disease by vaccine and non-vaccine types.
Browall S, Backhaus E, Naucler P, Galanis I, Sjöström K, Karlsson D, et al
Eur. Respir. J. 2014 Dec;44(6):1646-57

Secretion of a pneumococcal type II secretion system pilus correlates with DNA uptake during transformation.
Balaban M, Bättig P, Muschiol S, Tirier S, Wartha F, Normark S, et al
Proc. Natl. Acad. Sci. U.S.A. 2014 Feb;111(7):E758-65

Sinusitis and pneumonia hospitalization after introduction of pneumococcal conjugate vaccine.
Lindstrand A, Bennet R, Galanis I, Blennow M, Ask L, Dennison S, et al
Pediatrics 2014 Dec;134(6):e1528-36

Clinical efficacy of polyspecific intravenous immunoglobulin therapy in patients with streptococcal toxic shock syndrome: a comparative observational study.
Linnér A, Darenberg J, Sjölin J, Henriques-Normark B, Norrby-Teglund A
Clin. Infect. Dis. 2014 Sep;59(6):851-7

Evolutionary pathway to increased virulence and epidemic group A Streptococcus disease derived from 3,615 genome sequences.
Nasser W, Beres S, Olsen R, Dean M, Rice K, Long S, et al
Proc. Natl. Acad. Sci. U.S.A. 2014 Apr;111(17):E1768-76

Emergence of hypervirulent mutants resistant to early clearance during systemic serotype 1 pneumococcal infection in mice and humans.
Syk A, Norman M, Fernebro J, Gallotta M, Farmand S, Sandgren A, et al
J. Infect. Dis. 2014 Jul;210(1):4-13

Prevalence of community-acquired bacteraemia in Guinea-Bissau: an observational study.
Isendahl J, Manjuba C, Rodrigues A, Xu W, Henriques-Normark B, Giske C, et al
BMC Infect. Dis. 2014 Dec;14():3859

Improvement of CRB-65 as a prognostic tool in adult patients with community-acquired pneumonia.
Dwyer R, Hedlund J, Henriques-Normark B, Kalin M
BMJ Open Respir Res 2014 ;1(1):e000038

UlaR activates expression of the ula operon in Streptococcus pneumoniae in the presence of ascorbic acid.
Afzal M, Shafeeq S, Henriques-Normark B, Kuipers O
Microbiology (Reading, Engl.) 2015 Jan;161(Pt 1):41-9

European enhanced surveillance of invasive pneumococcal disease in 2010: data from 26 European countries in the post-heptavalent conjugate vaccine era.
Navarro Torné A, Dias J, Quinten C, Hruba F, Busana M, Lopalco P, et al
Vaccine 2014 Jun;32(29):3644-50

A systematic and functional classification of Streptococcus pyogenes that serves as a new tool for molecular typing and vaccine development.
Sanderson-Smith M, De Oliveira D, Guglielmini J, McMillan D, Vu T, Holien J, et al
J. Infect. Dis. 2014 Oct;210(8):1325-38

Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae.
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, et al
MBio 2014 Feb;5(1):e01120-13

The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold.
Schulte T, Löfling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, et al
Open Biol 2014 Jan;4():130090

Clinical utility of PCR for common viruses in acute respiratory illness.
Rhedin S, Lindstrand A, Rotzén-Östlund M, Tolfvenstam T, Ohrmalm L, Rinder M, et al
Pediatrics 2014 Mar;133(3):e538-45

Focal targeting by human β-defensin 2 disrupts localized virulence factor assembly sites in Enterococcus faecalis.
Kandaswamy K, Liew T, Wang C, Huston-Warren E, Meyer-Hoffert U, Hultenby K, et al
Proc. Natl. Acad. Sci. U.S.A. 2013 Dec;110(50):20230-5

Adult zebrafish model for pneumococcal pathogenesis.
Saralahti A, Piippo H, Parikka M, Henriques-Normark B, Rämet M, Rounioja S
Dev. Comp. Immunol. 2014 Feb;42(2):345-53

Intraclonal variations among Streptococcus pneumoniae isolates influence the likelihood of invasive disease in children.
Browall S, Norman M, Tångrot J, Galanis I, Sjöström K, Dagerhamn J, et al
J. Infect. Dis. 2014 Feb;209(3):377-88

Dominant role of nucleotide substitution in the diversification of serotype 3 pneumococci over decades and during a single infection.
Croucher N, Mitchell A, Gould K, Inverarity D, Barquist L, Feltwell T, et al
PLoS Genet. 2013 ;9(10):e1003868

Immunomodulatory effects of vitamin D on innate and adaptive immune responses to Streptococcus pneumoniae.
Olliver M, Spelmink L, Hiew J, Meyer-Hoffert U, Henriques-Normark B, Bergman P
J. Infect. Dis. 2013 Nov;208(9):1474-81


Older Publications

Publications 2007-2011

Publications 2006-2007 (Pdf file, 41 Kb)

Publications 1987-2005

Publication 1987-2005 (Pdf file, 59 Kb)

Group Members

Ingrid AnderssonAssociated

Marie-Stephanie aschtgenAssistant professor

Eleni BletsaPostdoc

073-372 87 20

Nils BlockGraduate Student

Karin BlomqvistAssistant professor

John BossBioinformatician

Maria CarlssonCoordinator

08-517 712 14

Hannes EichnerPhD student, Graduate Student

08-517 712 11

Margareta FlockResearch engineer

08-524 871 53

Jan-Ingmar FlockProfessor, senior

Genevieve GarrissAssistant professor

Felix GeisselPhD student, Graduate Student

Birgitta Henriques NormarkProfessor/senior physician

08-517 712 16

Karina HentrichPostdoc

Federico IovinoAssistant professor

Jens Karlsson PhD student, Graduate Student

Edmund LohSenior researcher

Peter MellrothSenior researcher

08-517 712 09

Padryk MerklPhD student, Graduate Student

Sandra MuschiolSenior lab manager

08-517 712 09

Priyanka NannapaneniBioinformatician

Staffan NormarkProfessor, senior

Vitor OliveiraPhD student, Graduate Student

Anuj PathakPostdoc

Alexandros PetropoulosAssociated

Elisabeth ReithuberPhD student, Graduate Student

Francesco RighettiPostdoc

Vicky SenderPostdoc

08-517 712 18

Georgios SotiriouAssistant professor

Christian SpoerryPostdoc

Karthik SubramanianPostdoc

Sigrún ThórsdóttirPhD student, Graduate Student

Vasiliki TsikourkitoudiPostdoc

Jill ZiesmerPhD student, Graduate Student

Ana-Rita NarcisoPostdoc

Associated Members

Katrin Pütsep

Awards and Prizes

Appointed Wallenberg Clinical Scholars 2017
Elected Member of the American Academy of Microbiology 2015
Elected Member of the European Academy of Microbiology 2013