Skip to main content

BD FACS Symphony analysis instrument at KI

The MedH Flow Cytometry Core Facility is pleased to announce that we have been able to acquire the first BD FACS Symphony analysis instrument in Sweden. To allow all interested investigators a unique opportunity to test the instrument, it will be available free of charge during a honeymoon period from September 10th to September 24th.

What is BD FACS Symphony instrument?

This is a state-of-the-art flow cytometry instrument with capacity for simultaneous high-throughput analysis of up to 50 parameters on single cells. This new platform allows deep profiling of complex tissues and populations based on the expression of cell surface and intracellular proteins.

The current configuration and available commercial reagents allow analysis of up to 30 parameters using 5 high-power lasers: 637nm, 488nm, 561nm, 405nm and 355nm. (For more details and updates regarding instrument configuration please visit: Flow Cytometry). The new BD FACS Symphony instrument also provides a complementary platform for advanced flow cytometry to our current BD FACS LSR Fortessa with ability to analyse up to 20 parameters.

Financing

The acquisition of the BD FACS Symphony analysis instrument was made possible through a StratRegen Infrastructure grant awarded to the MedH Flow Cytometry Core Facility and as such the subsidized user fees will be used to cover the service contract of the instrument. Importantly, the establishment and validation of such advanced instrumentation is done in close collaboration with several research groups with strong track record in flow cytometry at the Center for Hematology and Regenerative Medicine (HERM), as well as the Center for Infectious Medicine (CIM).

Superusers

In addition to the staff in the MedH Flow Cytometry Core Facility, the new BD FACS Symphony instrument has two Superusers who has taken the lead on the validation and advanced operation of the instrument: Heinrich Schlums (Bryceson lab) and Ece Somuncular (Luc lab).

Training and seminar

Although the operation of the BD FACS Symphony is very similar to the BD FACS LSR Fortessa, users are required to go through a brief training session on the Symphony before independent operation is allowed. In light of the ability to perform more complex flow cytometry analysis, the facility is also kicking off the availability of the new BD FACS Symphony instrument with a seminar by Dr. Mike Stadnisky, the CEO of FlowJo and an expert of analysis of complex flow cytometry data.

The seminar, entitled “Combining FACS, High-Resolution Mass-spectrometry, and Single Cell Sequencing for Social Network Analysis of Immune Cells” will take place on Wednesday 5 Sep, 11.00-12.00 at the Karolinska Institutet, NEO building*, room 5108 (Gene), 5th floor, Huddinge Campus. The seminar is open to all investigators at KI.
*Blickagången 16, Campus Flemingsberg, Karolinska Institutet Huddinge.