Zebrafish core facility - equipment and techniques

The zebrafish core facility at KI provides a wide selection of specialized equipment, techniques and high-containment biosafety laboratories for zebrafish research.

Techniques and equipment at a glance:

  • Working stations for microinjection
  • Epi-fluorescent stereomicroscopes
  • Working stations for behavior analysis
  • Compound microscope for high throughput screening
  • Cryopreservation service
  • BSL2-high containment laboratory
  • SPF unit for pathogen-free zebrafish

Complete workstations for micromanipulation of zebrafish embryos

We provide a total of four complete workstations for micromanipulation of zebrafish embryos. At these workstations, you are able to create new transgenic zebrafish lines, inject mRNA or DNA, transiently knock-down genes or xenotransplant cancer cells.

We can also train you in micromanipulation techniques or perform the experiments for you.

Epi-fluorescent stereomicroscopes

Microinjection at the zebrafish core facility.

Our epi-fluorescent stereomicroscopes enable you to image your zebrafish embryos in bright-field, dark-field and reverse contrast or take epi-fluorescent pictures. A heating plate ensures that the fish are kept at correct temperature during imaging.

We can assist you in how to optimally mount zebrafish embryos for your specific research project. 

Workstation for high-throughput analysis of zebrafish embryo behavior

We offer a Noldus DanioVision workstation and the EthoVision XT and Danioscope software for automated analysis of zebrafish behavior, heartbeats and blood flow.

We can assist you in the design and set-up for your experiment. Our equipment allows you to analyze the behavior of up to 96 embryos at the same time - ideal for screening purposes.

Compound microscope for high-throughput imaging of zebrafish embryos 

The zebrafish core facility provides a platform for automated imaging of zebrafish embryos. This platform, the ImageXpress Nano, is a plate-based imaging system in which zebrafish embryos (live/fixed) can be imaged in brightfield and or fluorescence.  Both single images and image stacks can be taken as well as time-laps and real-time movies recorded. The zebrafish core facility can assist you with mounting zebrafish embryos, image acquisition and image analysis. 

Documents

Cryopreservation of zebrafish lines 

We offer a cryopreservation service of zebrafish lines to both internal and external academic research groups. The cryopreservation service includes importing the zebrafish strain (if necessary), cryopreservation of sperm, storage of the sperm, in-vitro fertilization and export of rederived strains.

High-containment biosafety laboratory for zebrafish research

Our zebrafish core facility is one of the few facilities in the Nordic countries that offers a zebrafish Biosafety level-2 (BSL-2) laboratory. In this laboratory, you can expose zebrafish and other aquatic organisms to level-2 bacteria and viruses. The BSl-2 laboratory is equipped with a workstation for micromanipulation of embryos, an advanced epi-fluorescent microscope and a class-2 LAF hood.

SPF unit for zebrafish research

Our zebrafish core facility will be one of the few facilities in Europe that can provide certified SPF zebrafish free of Mycobacteria spp. and Pseudoloma spp. These animals are especially important for behavior assays and other experiments that require a high level of standardization. 

Open-access unit

Our open-access unit is accessible to our users at 24/7. Here users can work with their strains, set up breedings and run experiments on embryos and adults.

The unit is equipped with two activeBlue stand alone units, an epifluorescent stereomicroscope, a complete injection setup incubators and much more.

Light-sheet microscopy of zebrafish embryos

In collaboration with the Advanced Light Microscopy (ALM) Facility at Scilifelab we provide high-end in-vivo and real-time imaging of zebrafish embryos. An image from one of our recent projects employing light-sheet real-time microscopy was used on the cover page of the journal Neuro-Oncology.

KM
Komparativ Medicin
2022-01-19