Regional Multiple Sclerosis Centre, AOU San Luigi Gonzaga Orbassano, Turin, Italy

The Clinical Neurobiology Laboratory of the Regional Referring Multiple Sclerosis Centre (CRESM) is located at Neuroscience Institute Cavalieri Ottolenghi (NICO), University Hospital San Luigi Gonzaga, Orbassano, Turin (Italy). The laboratory deals with routine cerebrospinal fluid analysis and oligoclonal bands detection from AOU San Luigi Gonzaga patients and from several Piedmont centres.

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Additionally, detection of anti-Aquaporin 4 and anti-Myelin Oligodendrocyte Glycoprotein antibodies on serum samples by immunofluorescence assay and FACS assay respectively are performed for differential diagnosis with Neuromyelitis Optica Spectrum Disorders. Furthermore, the lab is focused on the evaluation of treatment-response biomarkers in MS treated patients to monitor the biological response to therapy and identify risk factors

Diseases Researched:

The main diseases that this lab researches are:

  • Multiple Sclerosis
  • Neuromyelitis Optica Spectrum Disorders

Performed Tests:

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Drug serum levels

  • Measure rituximab with commercial ELISA Kit
  • Measures natalizumab with home-made flow cytometry assay

ADA titres

  • Measuring anti-IFNβ antibodies with Cytopathic Effect (CPE) assay
  • Measuring anti-natalizumab antibodies with bridging ELISA
  • Measuring anti-rituximab antibodies with commercial ELISA Kit

IFNβ biological activity

  • Quantification of MxA mRNA with quantitative real time PCR

Rituximab monitoring

  • Quantification of CD19 mRNA with droplet digital PCR

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