Genetic analysis
KIGene is a well-established node at Karolinska Institutet with more than thirty years experiences of running a core facility. We offer genetic analyses, covering DNA sequencing, gene expression, genotyping, miRNA profiling and copy number variation, as well as epigenetic analysis and digital spatial profiling of RNA and protein on tissue slides.
KIGene is open to academic and non-academic users from Sweden and abroad.
Aim
Our vision is to provide a high quality, reliable, high efficiency and flexible core facility. In order to meet the increasing demand on higher throughput and efficiency, as well as on cheaper analyses, we have introduced several options for gene expression and genetic variations, including single nucleotide polymorphisms (SNPs), microsatellite markers, insertion/deletion polymorphisms and copy number variation.
Flexible service
"Hands-on" service: The user receives a short introduction by experienced personnel regarding sample preparation and operation of the instruments. Each user reserves a date and time for occupying an instrument by a booking system.
"Hands-off" service: The user provides us with pre-made sequence or fragment analysis reactions and we are performing the analysis. The resulting files are transferred to each user by e-mail.
Full-service: The user provides us with starting materials- as saliva, biopsies, DNA, oligonucleotides- and we are performing the analysis. The resulting files are transferred to each user by e-mail.
We provide:
- Sanger DNA sequencing services with turn-around time within the same day
- Genotyping (SNP and fragment length analysis)
- Gene expression analysis
- Copy number variation
- Rare target detection
- miRNA profiling
- DNA methylation analysis
- Quantity and quality determination of RNA, DNA, protein and dye incorporation
- User support and training courses
Instruments and applications
ABI 3730 PRISM® DNA Analyzers
for
- Sanger DNA sequencing with sample-to-data within the same day
- Fragment length analysis
Agilent 2100 Bioanalyzer, NanoDrop 1000 and Qubit 2.0
for
- quantity and quality control of DNA, RNA and proteins
nanoString nCounter® analysis system
for
- gene expression analysis
- miRNA profiling
- copy number analysis
- read-out of GeoMx digital spatial profiling data
QuantStudio 3D Digital PCR System
for
- SNP analysis
- gene expression analysis
- rare target detection
QuantStudio 6 and 7 FLEX instruments
for
- SNP analysis by dissociation analysis or allelic discrimination
- gene expression analysis.
Pyromark Q96 ID instrument
-quantitative analysis of genetic or epigenetic DNA modifications using Pyrosequencing technology
Contact Information
Address
KIGene, MMK, Translational Psychiatry, CMM L8:00, Karolinska University Hospital at Solna, 171 76 Stockholm.