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Chunyan Zhao

Senior researcher

Visiting address : (Bionut), H2, Novum, Hälsovägen 7 (By Car), Blickagången 6 (By Train) 141 57 Huddinge, Sweden
Postal address : Department of Biosciences and Nutrition (BioNut), H2, Dahlman-Wright, (Bionut), H2 141 83 Huddinge, Sweden
Delivery address : (Bionut), H2, Novum, Hälsovägen 7-9, Hiss D, Plan 7 141 57 Huddinge, Sweden

About me

I am an Associate Professor (“Docent” in the Swedish academic system) and acting group leader for the ESR (Estrogen Signaling Research) group. I received my PhD from Karolinska Institutet in 2000, under the supervision of Professor Kari Hemminki. My PhD project studied the molecular mechanisms of carcinogenesis mediated by chemical carcinogens or UV-radiation.  In 2002, I joined the ESR group, focusing at understanding estrogen signaling and AP-1 signaling in breast cancer using functional genomics approaches.

Research description

Functional genomics of breast Cancer

Patients with estrogen receptor (ER)-positive breast cancer are usually treated with anti-hormone therapies such as tamoxifen or aromatase inhibitors. However, many of these patients are resistant to these drugs or develop resistance during treatment, allowing their breast cancer to return. In addition, patients with triple-negative breast cancer (TNBC) have limited treatment options. Our group is using functional genomics approaches towards unravelling mechanisms of drug resistance in ER-positive breast cancer and understanding molecular determinants of malignant cell behaviors in TNBC. The ultimate goal is to develop novel and improved prognostic tools and therapies for patients with invasive breast tumors.

Recent published results from the group showed the first evidence that the AP-1 transcription factor Fra-1 is overexpressed in TNBC and has prognostic value. This work provided novel insights into the mechanisms through which TNBC cells acquire invasive and proliferative properties. Currently there are three main projects in focus 1) Characterization of the role of AP-1 in regulating TNBC invasiveness, in breast cancer progression and metastasis and in breast cancer stem cells. 2) Identification of the ER cistrome associated proteome in response to different ligands in breast cancer cells. With the term “the ER cistrome associated proteome” we refer to the global identification of proteins associated with primarily the DNA bound ER. 3) Identification of the ER cistrome associated proteome in tamoxifen resistant compared to tamoxifen sensitive breast cancer.

 

Publications

AP-1 Is a Key Regulator of Proinflammatory Cytokine TNFα-mediated Triple-negative Breast Cancer Progression
Qiao Y, He H, Jonsson P, Sinha I, Zhao C, Dahlman-wright K
The Journal of biological chemistry 2016;291(10):5068-79

Blockade of the Hedgehog pathway downregulates estrogen receptor alpha signaling in breast cancer cells
Diao Y, Azatyan A, Rahman Mf, Zhao C, Zhu J, Dahlman-wright K, et al
Oncotarget 2016;7(44):71580-71593

Estrogen Receptor α Promotes Breast Cancer by Reprogramming Choline Metabolism
Jia M, Andreassen T, Jensen L, Bathen Tf, Sinha I, Gao H, et al
Cancer research 2016;76(19):5634-5646

RING finger protein 31 promotes p53 degradation in breast cancer cells
Zhu J, Zhao C, Zhuang T, Jonsson P, Sinha I, Williams C, et al
Oncogene 2016;35(15):1955-64

AP-1-mediated chromatin looping regulates ZEB2 transcription: new insights into TNFα-induced epithelial-mesenchymal transition in triple-negative breast cancer
Qiao Y, Shiue Cn, Zhu J, Zhuang T, Jonsson P, Wright Ap, et al
Oncotarget 2015;6(10):7804-14

Induction of USP17 by combining BET and HDAC inhibitors in breast cancer cells
Borbely G, Haldosen La, Dahlman-wright K, Zhao C
Oncotarget 2015;6(32):33623-35

p21-activated kinase group II small compound inhibitor GNE-2861 perturbs estrogen receptor alpha signaling and restores tamoxifen-sensitivity in breast cancer cells
Zhuang T, Zhu J, Li Z, Lorent J, Zhao C, Dahlman-wright K, et al
Oncotarget 2015;6(41):43853-68

Estrogen receptor beta in breast cancer
Haldosén La, Zhao C, Dahlman-wright K
Molecular and cellular endocrinology 2014;382(1):665-72

Genome-wide profiling of AP-1-regulated transcription provides insights into the invasiveness of triple-negative breast cancer
Zhao C, Qiao Y, Jonsson P, Wang J, Xu L, Rouhi P, et al
Cancer research 2014;74(14):3983-94

The atypical ubiquitin ligase RNF31 stabilizes estrogen receptor α and modulates estrogen-stimulated breast cancer cell proliferation
Zhu J, Zhao C, Kharman-biz A, Zhuang T, Jonsson P, Liang N, et al
Oncogene 2014;33(34):4340-51

Expression of activator protein-1 (AP-1) family members in breast cancer
Kharman-biz A, Gao H, Ghiasvand R, Zhao C, Zendehdel K, Dahlman-wright K
BMC cancer 2013;13():441-

GENE EXPRESSION OF ESTROGEN RECEPTORS IN PBMC FROM PATIENTS WITH PUUMALA VIRUS INFECTION
Brundin P, Zhao Cy, Dahlman-wright K, Ahlm C, Evengard B
SHOCK 2012;37(4):355-9

Global identification of genes regulated by estrogen signaling and demethylation in MCF-7 breast cancer cells
Putnik M, Zhao C, Gustafsson JÅ, Dahlman-wright K
Biochemical and biophysical research communications 2012;426(1):26-32

Interplay between AP-1 and estrogen receptor α in regulating gene expression and proliferation networks in breast cancer cells
Dahlman-wright K, Qiao Y, Jonsson P, Gustafsson JÅ, Williams C, Zhao C
Carcinogenesis 2012;33(9):1684-91

The diversity of sex steroid action: regulation of metabolism by estrogen signaling
Faulds Mh, Zhao Cy, Dahlman-wright K, Gustafsson Ja
JOURNAL OF ENDOCRINOLOGY 2012;212(1):3-12

The Transcription Factor Encyclopedia
Yusuf D, Butland Sl, Swanson Mi, Bolotin E, Ticoll A, Cheung Wa, et al
GENOME BIOLOGY 2012;13(3):R24-

Estrogen Signaling via Estrogen Receptor beta
Zhao Cy, Dahlman-wright K, Gustafsson Ja
JOURNAL OF BIOLOGICAL CHEMISTRY 2010;285(51):39575-9

Fatty Acids Derived from Royal Jelly Are Modulators of Estrogen Receptor Functions
Moutsatsou P, Papoutsi Z, Kassi E, Heldring N, Zhao Cy, Tsiapara A, et al
PLOS ONE 2010;5(12):e15594-

Gene Expression Profiling and Chromatin Immunoprecipitation Identify DBN1, SETMAR and HIG2 as Direct Targets of SOX11 in Mantle Cell Lymphoma
Wang X, Bjorklund S, Wasik Am, Grandien A, Andersson P, Kimby E, et al
PLOS ONE 2010;5(11):e14085-

Genome-Wide Mapping of Estrogen Receptor-beta-Binding Regions Reveals Extensive Cross-Talk with Transcription Factor Activator Protein-1
Zhao Cy, Gao H, Liu Yw, Papoutsi Z, Jaffrey S, Gustafsson Ja, et al
CANCER RESEARCH 2010;70(12):5174-83

Liver X receptor in cholesterol metabolism
Zhao Cy, Dahlman-wright K
JOURNAL OF ENDOCRINOLOGY 2010;204(3):233-40

Molecular biology and functional genomics of liver X receptors (LXR) in relationship to metabolic diseases
Faulds Mh, Zhao Cy, Dahlman-wright K
CURRENT OPINION IN PHARMACOLOGY 2010;10(6):692-7

Molecular characterization of estrogen receptor signaling in breast cancer cells
Zhao C, Gao H, Gustafsson N, Papoutsi Z, Jaffrey S, Gustafsson Ja, et al
FEBS JOURNAL 2010;:10-10

RBCK1 Drives Breast Cancer Cell Proliferation by Promoting Transcription of Estrogen Receptor alpha and Cyclin B1
Gustafsson N, Zhao Cy, Gustafsson Ja, Dahlman-wright K
CANCER RESEARCH 2010;70(3):1265-74

Binding of estrogen receptor alpha/beta heterodimers to chromatin in MCF-7 cells
Papoutsi Z, Zhao Cy, Putnik M, Gustafsson Ja, Dahlman-wright K
JOURNAL OF MOLECULAR ENDOCRINOLOGY 2009;43(1-2):65-72

Effects of two common polymorphisms in the 3 ' untranslated regions of estrogen receptor beta on mRNA stability and translatability
Putnik M, Zhao C, Gustafsson Ja, Dahlman-wright K
BMC GENETICS 2009;:55-

Microarray analysis of altered gene expression in ER beta-overexpressing HEK293 cells
Zhao Cy, Putnik M, Gustafsson Ja, Dahlman-wright K
ENDOCRINE 2009;36(2):224-32

Tumor Repressive Functions of Estrogen Receptor beta in SW480 Colon Cancer Cells
Hartman J, Edvardsson K, Lindberg K, Zhao Cy, Williams C, Strom A, et al
CANCER RESEARCH 2009;69(15):6100-6

Estrogen receptor beta: an overview and update
Zhao C, Dahlman-wright K, Gustafsson Ja
Nuclear receptor signaling 2008;6():e003-

Estrogen receptor beta 2 negatively regulates the transactivation of estrogen receptor alpha in human breast cancer cells
Zhao Cy, Matthews J, Tujague M, Wan J, Strom A, Toresson G, et al
CANCER RESEARCH 2007;67(8):3955-62

Functional characterization of a novel variant of estrogen receptor beta identified in screening of DNA derived from African Americans
Zhao C, Gustafsson Ja, Dahlman-wright K
PHARMACOGENETICS AND GENOMICS 2006;16(5):379-83

Mouse estrogen receptor beta isoforms exhibit differences in ligand selectivity and coactivator recruitment
Zhao Cy, Toresson G, Xu L, Koehler Kf, Gustafsson Ja, Dahlman-wright K
BIOCHEMISTRY 2005;44(22):7936-44

Identification of a functional variant of estrogen receptor beta in an African population
Zhao Cy, Xu L, Otsuki M, Toresson G, Koehler K, Pan-hammarstrom Q, et al
CARCINOGENESIS 2004;25(11):2067-73

Expression of estrogen receptor beta isoforms in normal breast epithelial cells and breast cancer: regulation by methylation
Zhao Cy, Lam Ewf, Sunters A, Enmark E, De Bella Mt, Coombes Rc, et al
ONCOGENE 2003;22(48):7600-6

Polymorphisms in the estrogen receptor beta gene and risk of breast cancer: no association
Forsti A, Zhao Cy, Israelsson E, Dahlman-wright K, Gustafsson Ja, Hemminki K
BREAST CANCER RESEARCH AND TREATMENT 2003;79(3):409-13

Single nucleotide polymorphisms in the XPG gene: Determination of role in DNA repair and breast cancer risk
Kumar R, Hoglund L, Zhao C, Forsti A, Snellman E, Hemminki K
INTERNATIONAL JOURNAL OF CANCER 2003;103(5):671-5

Demonstration of UV-dimers in human skin DNA in situ 3 weeks after exposure
Hemminki K, Xu Gg, Kause L, Koulu Lm, Zhao Cy, Jansen Ct
CARCINOGENESIS 2002;23(4):605-9

In situ repair of cyclobutane pyrimidine dimers in skin and melanocytic nevi of cutaneous melanoma patients
Zhao Cy, Snellman E, Jansen Ct, Hemminki K
INTERNATIONAL JOURNAL OF CANCER 2002;98(3):331-4

Measurement of cyclobutane thymidine dimers in melanocytic nevi and surrounding epidermis in human skin in situ
Wilms Lc, Zhao C, Snellman E, Segerback D, Hemminki K
MUTAGENESIS 2002;17(3):189-91

The in vivo levels of DNA alkylation products in human lymphocytes are not age dependent: an assay of 7-methyl- and 7-(2-hydroxyethyl)-guanine DNA adducts
Zhao Cy, Hemminki K
CARCINOGENESIS 2002;23(2):307-10

Ultraviolet photoproduct levels in melanocytic nevi and surrounding epidermis in human skin in situ
Zhao Cy, Snellman E, Jansen Ct, Hemminki K
JOURNAL OF INVESTIGATIVE DERMATOLOGY 2002;118(1):180-4

DNA adducts as a marker for cancer risk?
Hemminki K, Koskinen M, Zhao Cy
INTERNATIONAL JOURNAL OF CANCER 2001;92(6):923-6

DNA adducts of 1,3-butadiene in humans: Relationships to exposure, GST genotypes, single-strand breaks, and cytogenetic end points
Zhao C, Vodicka P, Sram Rj, Hemminki K
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS 2001;37(3):226-30

DNA adducts, mutations, and cancer 2000
Hemminki K, Koskinen M, Rajaniemi H, Zhao Cy
REGULATORY TOXICOLOGY AND PHARMACOLOGY 2000;32(3):264-75

Human DNA adducts of 1,3-butadiene, an important environmental carcinogen
Zhao Cy, Vodicka P, Sram Rj, Hemminki K
CARCINOGENESIS 2000;21(1):107-11

P-32-postlabelling analysis of 1,3-butadiene-induced DNA adducts in vivo and in vitro
Zhao Cy, Koskinen M, Hemminki K
BIOMARKERS 2000;5(3):168-81

Comparison of P-32-postlabeling and high-resolution GC/MS in quantifying N7-(2-hydroxyethyl)guanine adducts
Eide I, Zhao Cy, Kumar R, Hemminki K, Wu Ky, Swenberg Ja
CHEMICAL RESEARCH IN TOXICOLOGY 1999;12(10):979-84

Endogenous and background DNA adducts by methylating and 2-hydroxyethylating agents
Zhao Cy, Tyndyk M, Eide I, Hemminki K
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 1999;424(1-2):117-25

32P-postlabelling of N6-adenine adducts of epoxybutanediol in vivo after 1,3-butadiene exposure
Zhao C, Koskinen M, Hemminki K
Toxicology letters 1998;102-103():591-4

Measurement of 7-methyl- and 7-(2-hydroxyethyl)guanine DNA adducts in white blood cells of smokers and non-smokers
Zhao Cy, Kumar R, Hemminki K
BIOMARKERS 1998;3(4-5):327-334

P-32-postlabelling of N-6-adenine adducts of epoxybutanediol in vivo after 1,3-butadiene exposure
Zhao Cy, Koskinen M, Hemminki K
TOXICOLOGY LETTERS 1998;:591-594

DNA adducts in human nasal mucosa and white blood cells from smokers and non-smokers
Zhao Cy, Georgellis A, Flato S, Palmberg L, Thunberg E, Hemminki K
CARCINOGENESIS 1997;18(11):2205-8

Persistence of 7-(2-hydroxyethyl)guanine-DNA adducts in rats exposed to ethene by inhalation
Zhao Cy, Kumar R, Zahlsen K, Sundmark Hb, Hemminki K, Eide I
BIOMARKERS 1997;2(6):355-359

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