2nd StratCan workshop on CRISPR/Cas9
Theory & hands on workshop to design and use the CRISPR/Cas9 system for in vitro (cells) and in vivo (mice) applications.
The workshop takes place January 12-13, 2016 at KI Science Park, room Air & Fire (address: Tomtebodavägen 23 A).
- Feng Zhang, Broad Institute of MIT
- Oscar Fernandez-Capetillo, Spanish National Cancer Research Center and KI
- Philip Hublitz, University of Oxford, WIMM
- Thoru Pederson, University of Massachusetts Medical School
- Vera Grinkevich, Wellcome Trust Sanger Institute
The workshop is composed of:
A. Theory / Background
B. In vitro use (cells)
C. In vivo use (mice)
The final program will be tailor made according to the participants needs.
TUESDAY JAN 12
09.00 – 09.30 Galina Selivanova, Maria Kasper
Welcome & workshop introduction.
09.30 – 10.30 Feng Zhang
Introduction to Genome Editing Using CRISPR-Cas9.
10.30 – 11.00 COFFEE
11.00 – 12.00 Oscar Fernandez-Capetillo
Genome-wide loss-of-function screening using the CRISPR-Cas9 system.
12.00 – 13.00 LUNCH
13.00 – 14.00 Vera Grinkevich
Genome-wide CRISPR screens to unveil drug resistance in cancers.
14.00 – 15.30 Madhurendra Singh & Vera Grinkevich
Supervising hands on training of participants to use web-based tools.
15.30 – 17.00 Feng Zhang & Philip Hublitz
Discussion group: Advances and challenges of CRISPR/Cas9.
17.30 Dinner at Faculty club Svarta Rävfen, Aula Medica
WEDNESDAY JAN 13
09.00 – 09.55 Philip Hublitz
A Core-Facility's Perspective on CRISPR-Cas9 Genome Engineering in Mice and Cells.
09.55 – 10.05 COFFEE served
10.05 – 11.00 Feng Zhang
Advanced Application of CRISPR-Cas9 for Genome Perturbation.
11.00 – 12.00 Thoru Pederson
CRISPR in a Rainbow of Colors and Opportunities, Advancing Basic and Clinical Research.
12.00 – 13.00 LUNCH
13.00 – 15.00 Teachers and workshop participants
In-depth discussion and hands on design to generate mouse models
Ex. Crisp/cas9 system to create point mutation, Cre/flox and reporter mice
Registration is closed (Deadline December 1st 2015) and the workshop is full. Selected participants will be notified shortly (Dec 9th/10th).
The number of participants is limited to 40 (priority will be given to KI applicants) and a 650 SEK registration fee will apply (payment details will be provided with the acceptance notice). Please note that attendance during the full 2-day workshop is required.