- 2002 - 2005: BSc in Molecular Biotechnology at the Universität zu Lübeck, Germany
- 2005 - 2007: MSc in Biomedical Sciences, Leiden University Medical Center, Leiden, The Netherlands, and Howard Hughes Medical Institute & Department of Biology, University of Pennsylvania, Philadelphia, PA, USA.
- 2008 - 2012: Ph.D. with Prof. Hans Jörnvall, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. Thesis: “Molecular mechanisms of amyloid regulation”
- 2013 - 2014: Postdoctoral fellow with Prof. Jan Johansson, Department of Neuroscience, and Prof. Hans Jörnvall, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
- 2014 - 2016: Marie Curie Career Development Fellow with Prof. Dame Carol Robinson FRS, Department of Chemistry, University of Oxford, UK
- Since 2017: Assistant Professor in Mass Spectrometry in the group of Prof. Sir David Lane FRS, Department of Microbiology, Tumor and Cell Biology, Science for Life Laboratory, Karolinska Institutet, Stockholm, Sweden
Mass spectrometry of protein interactions from cancer to memory
All biological processes can be described as biomolecules “talking” to each other, providing cargo, information, or transportation. These events usually take the from of a direct physical contact, i.e. a non-covalent interaction, in which one molecule, most often a protein, binds to one or more partners, inducing a change in the three-dimensional structure. In this manner, proteins can keep in touch with their environment to control their function. For example, upon sensing a change in pH, sider silk proteins lock each other into infinite chains to form very stable scaffolds, and membrane proteins can recognise individual lipid molecules in their environment to tune their activity accordingly. Aberrant, -faulty- interactions, on the other hand, interfere with these processes and are therefore often associated with diseases. Some proteins interact with themselves and form toxic structures such as amyloid fibrils that eventually lead to degeneration of the affected tissue, as seen in e.g Alzheimer's disease. Similaly, destabilization and aggregation of the tumour suppressor p53 and its targets leads loss of cell cycle control and impaired DNA damage repair, giving rise to cancer. Therefore, it is important to understand how exactly proteins “talk” to each other, and use this information to find ways to prevent interactions from going wrong.
My research focuses on the use of mass spectrometry (MS), a technique which allows us to determine the exact weight of biomolecules, to study how proteins recognise and bind their partners. MS is well-suited for the study of transient interactions, large complexes and even unstable proteins, all of which are refractory to other structural biology methods like NMR and X-ray crystallography. For this purpose, we combine several complementary approaches:
- In “native” MS, we gently transfer proteins together with their binding partners from physiological solutions into the vacuum inside the mass spectrometer and measure the weight and stability of the resulting complex. This reveals what type of interaction holds the partners together, and how many (and which) molecules are involved.
- Hydrogen/deuterium exchange MS measures the incorporation of a chemical label (Deuterium) into the protein. Deuterium is incorporated into flexible and exposed parts of the protein. By measuring the resulting increase in weight, we are able to determine the stability and folding state of a protein, and even locate binding sites for tother proteins.
- MS-based proteomics allows us to identify individual proteins from complex mixtures based on their unique mass “fingerprints”. Using individual proteins as bait, we are able to fish out their specific interaction partners and map upstream and downstream targets.
The combination of all three techniques provides direct insights into several aspects of an interaction, but also generates constraints that can be used to direct computational modelling.
Monitoring intracellular interactions for drug development. Protein-protein interactions are commonly studied in vitro using isolated, purifed proteins. We are combining intracellular HDX-MS and native MS to preserve conformational signatures of protein complexes inside bacterial cells for read-out by MS. Using the interactions between the tumor suppressor p53 and its downstream partners as a target system, we want to follow e.g. the effects of drugs on protein-protein interactions in a cellular environment. The goal is to integrate intracellular structural biology with proteome-wide screening methods for drug target engagement such as CETSAs that can be applied in targeted drug discovery.
Structure, stabilty, and interactions of prion-like proteins in memory formation. Aggregation-prone proteins are related to a number of diseases such as neurodegenerative disorders, hereditary cancers, and interstitial lung disease, yet remain among the most challenging targets for structural biology. We are using the power of structural mass spectrometry to understand how the ability of a group of prion-like proteins to assemble into large cellular structures facilitates long-term memory formation.
The aims of this project are two-fold: 1. We want to unravel the role of each part of these complex multi-domain proteins in switching from a soluble, inactive state to an amyloid-like structure without undegoing toxic, uncontrolled aggregation normally observed for prion proteins. 2. We are interested in how self-assembly affects the interactome of memnons, such as their ability to bind RNAs and anti-amyloid chaperones. Using MS-based structure and interaction maps, we hope to derive models for self-assembly and its impact on the cellular processes that can shed light on how structural changes in a small family of proteins can elicit something as complex as memory formation.
We also collaborate with David Drew (Stockholm U) on membrane protein-lipid interactions, Erik Marklund (Uppsala) on integrating MS and MD simulations, Carol Robinson (Oxford) on MS method development, and Jan Johansson (KI) and Anna Rising (SLU) on strategies against amyloid formation, and the biology of spider silk production.
Neurodegenerative diseases and protein aggregation disorders
- Liu, N., Landreh, M., Cao, K., Abe, M., Hendriks, J.-G., Kennerdell, J., Zhu, Y., Wang, L.S., Bonini, N.M. The microRNA miR-34 modulates ageing and neurodegeneration in Drosophila. Nature (2012) 482: 519-523.
- Willander, H.*, Askarieh, G.*, Landreh, M.*, Westermark, P., Nordling, K., Keränen, H., Hermansson, E., Hamvas, A., Nogee, L.M., Bergman, T., Saenz, A., Casals, C., Åqvist, J., Jörnvall, H., Berglund, H., Presto, J., Knight, S.D., Johansson, J. High-resolution structure of a BRICHOS domain. Implications for anti-amyloid chaperone activity on lung surfactant protein C. Proc. Natl. Acad. Sci. USA. (2012) 109:2325-2329.
- Landreh, M.,* Sawaya, M.R.,* Hipp, M.S.,* Eisenberg, D.S., Wüthrich, K., Hartl, F.U. The Formation, Function and Regulation of Amyloids: Insights from Structural Biology. J. Int. Med. (2016) 280: 164-176.
- Gupta, K.,* Donlan, J.,* Hopper, J.T.,* Uzdavinys, P., Landreh, M., Struwe, W., Drew, D., Stansfeld, P., Baldwin, A.J., Robinson, C.V. The role of interfacial lipids in stabilising membrane protein oligomers. Nature (2017) 541: 421-424.
- Landreh, M., Marklund, E.G., Uzdavinys, P., Degiacomi, M.T., Coincon, M., Gault, J., Gupta, K., Liko, I., Benesch, J.L.P., Drew, D., Robinson, C.V. Integrating mass spectrometry and MD simulations reveals the role of lipids in Na+/H+ antiporters. Nature Commun. (2017) 8: 13993
- Landreh, M., Marty, M.T., Gault, J., Robinson, C.V. A sliding selectivity scale for lipid binding to membrane proteins. Curr. Opin. Struct. Biol. (2016) 39: 54-60.
- Landreh, M.#, Askarieh, G., Nordling, K., Hedhammar, M., Rising, A., Astorga-Wells, J., Alvelius, G., Casals, C., Knight, S. D., Johansson, J., Jörnvall, H., and Bergman, T. A pH-dependent dimer lock in spider silk protein. J. Mol. Biol. (2010) 404:328-336.
- Andersson, M., Jia, Q., Abella, A., Lee, X.-Y., Landreh, M., Purhonen, P., Hebert, H., Tenje, M., Robinson, C.V., Meng, Q., Plaza, G., Johansson, J., Rising, A. Biomimetic spinning of artificial spider silk from a chimeric minispidroin. Nature Chem. Biol. (2017) 13: 262–264.
- Landreh, M., Andersson, M., Marklund, E.G., Jia, Q., Meng, Q., Johansson, J., Robinson, C.V., Rising, A. Mass spectrometry captures structural intermediates in protein fiber self-assembly. Chem. Commun. (2017) 53: 3319-3322.
- Astorga-Wells, J.*, Landreh, M.*, Bergman, T., Jörnvall, H. A membrane cell for online Hydrogen/Deuterium exchange to study protein folding and protein-protein interaction by mass spectrometry. Mol. Cell. Proteomics (2011) 10:M110.006510.
- Landreh, M., Liko, I., Uzdavinys, P., Coincon, M., Hopper, J.T.S., Drew, D., Robinson, C.V. Controlling release, unfolding and dissociation of membrane protein complexes in the gas phase through collisional cooling. Chem. Commun. (2015) 51: 15582-15584.
- Allison, T.M.,* Landreh, M.,* Benesch, J.L.P., Robinson, C.V. Low charge and reduced mobility of membrane protein complexes has implications for calibration of collision cross section measurements. Anal. Chem. (2016) 88: 5879-5884.
* Equal contribution
# Corresponding author
For a full publication list, please visit my Google Scholar page. (a.k.a. Michael Fitzen)
- 2004 - 2005 Lab courses and seminars in general and organic chemistry, medical program, University of Lübeck, Germany
- 2008 - 2014: Lab courses, seminars, lectures and examiner for General and Organic Chemistry (biomedical program), Physiological Chemistry (medical program) Laboratory safety (medical and biomedical programs), Department of MedicalBiochemistry and Biophysics, Karolinska Institutet
- 2014 - 2016: Academic Advisor for DPhil students, St Cross College, University of Oxford
- Since 2017: Lectures and Seminars, General and Organic Chemistry courses, Department of Medical Biochemistry and Biophysics, Karolinska Institutet
Academic honours, awards and prizes
- 2007: “Heart of Biomedical Science” Prize of the Leiden University Medical Student Association (M.F.L.S.)
- 2008-2012: Karolinska Ph.D. student scholarship (KID grant), 1.1 mSEK
- 2014-2016: ERC Marie Curie Early Career Development Fellowship in Life Sciences, 2.2 mSEK
- 2014-2016: Junior Research Fellowship, St. Cross College, Oxford
- 2017-2021: Ingvar Carlsson Award, 4 mSEK