Denna sida på svenskaAgneta Richter-Dahlfors

Agneta Richter Dahlfors


Visiting address : Retzius Väg 8 171 65 Solna, Sweden
Postal address : Department of Neuroscience (Neuro), C4, Department of Neuroscience (Neuro), C4, Retzius Väg 8 171 77 Stockholm, Sweden
Delivery address : Institutionen För Neurovetenskap, Karolinska Institutet, Retzius Väg 8 171 65 Solna, Sweden

Research description

Situated in the Swedish Medical Nanoscience Center, Richter-Dahlfors’ research group embraces scientists with expertise in micro­biology/infection, neuroscience, and medicine as well as chemistry, organic- and micro­electronics. Via extensive engagement in national and international multidisciplinary collaborations, we focus on defining the integrated pathophysiology of bacterial infections, and on generating novel technologies for neuromodulation.

Tissue microbiology reveals the integrated pathophysiology of infection: By applying a panel of intravital techniques (e.g. intravital 2-photon microscopy, amperometric bio­sensors), we study the real-time dynamics of the infection process within the organ at high resolution using uropathogenic E. coli-induced pyelonephritis as model.

Our work

  • aids in establishing the emerging field ‘tissue micro­biology’
  • advances the development of biomimetic tools for complementary in vitro studies
  • focuses on developing bio­sensors for hospital-acquired infections

Organic bioelectronics in nanomedicine: We recently demonstrated the use of conducting polymers for precise, non-convective, electrophoretic delivery of signal substances, such as ions and neurotransmitters. Analogously to the function of neurons, the device mimics the action potential causing release of chemical substances into the synaptic cleft. We currently focus on integrating this technology as a novel machine-to-brain interface and as an implantable neuromodulation technology.



An organic electronic biomimetic neuron enables auto-regulated neuromodulation
Simon Dt, Larsson Kc, Nilsson D, Burstrom G, Galter D, Berggren M, et al
Biosensors & bioelectronics 2015;71():359-64

Integrated Pathophysiology of Pyelonephritis
Choong Fx, Antypas H, Richter-dahlfors A
Microbiology spectrum 2015;3(5):-

Organic bioelectronics in infection
Loffler S, Libberton B, Richter-dahlfors A

Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity
Loffler S, Richter-dahlfors A

Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds
Alarcon Ei, Udekwu Ki, Noel Cw, Gagnon Lb, Taylor Pk, Vulesevic B, et al
Nanoscale 2015;7(44):18789-98

Bacterial nanoscale cultures for phenotypic multiplexed antibiotic susceptibility testing
Weibull E, Antypas H, Kjall P, Brauner A, Andersson-svahn H, Richter-dahlfors A
Journal of clinical microbiology 2014;52(9):3310-7

Biomimetic interfaces reveal activation dynamics of C-reactive protein in local microenvironments
Goda T, Kjall P, Ishihara K, Richter-dahlfors A, Miyahara Y
Advanced healthcare materials 2014;3(11):1733-8

Intravital two-photon imaging to understand bacterial infections of the mammalian host
Choong Fx, Richter-dahlfors A
Methods in molecular biology (Clifton, N.J.) 2014;1197():87-100

Polycaprolactone-thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells
Wickham Am, Islam Mm, Mondal D, Phopase J, Sadhu V, Tamas E, et al
Journal of biomedical materials research. Part B, Applied biomaterials 2014;102(7):1553-61

Organic bioelectronics for electronic-to-chemical translation in modulation of neuronal signaling and machine-to-brain interfacing
Larsson Kc, Kjall P, Richter-dahlfors A
Biochimica et biophysica acta 2013;1830(9):4334-44

Organic bioelectronics - novel applications in biomedicine. Preface
Owens R, Kjall P, Richter-dahlfors A, Cicoira F
Biochimica et biophysica acta 2013;1830(9):4283-5

Intravital models of infection lay the foundation for tissue microbiology
Choong Fx, Regberg J, Udekwu Ki, Richter-dahlfors A
Future microbiology 2012;7(4):519-33

Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo
Choong Fx, Sandoval Rm, Molitoris Ba, Richter-dahlfors A
Methods in enzymology 2012;506():35-61

Swedish Medical Nanoscience Center at Karolinska Institutet
Jacobson Gb, Richter-dahlfors A

The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles
Alarcon Ei, Udekwu K, Skog M, Pacioni Nl, Stamplecoskie Kg, Gonzalez-bejar M, et al
Biomaterials 2012;33(19):4947-56

Tissue microbiology provides a coherent picture of infection
Richter-dahlfors A, Rhen M, Udekwu K
Current opinion in microbiology 2012;15(1):15-22

Comparative tissue transcriptomics reveal prompt inter-organ communication in response to local bacterial kidney infection
Boekel J, Kallskog O, Ryden-aulin M, Rhen M, Richter-dahlfors A
BMC genomics 2011;12():123-

Electronic control of cell detachment using a self-doped conducting polymer
Persson Km, Karlsson R, Svennersten K, Loffler S, Jager Ew, Richter-dahlfors A, et al
Advanced materials (Deerfield Beach, Fla.) 2011;23(38):4403-8

Mechanical stimulation of epithelial cells using polypyrrole microactuators
Svennersten K, Berggren M, Richter-dahlfors A, Jager Ew
Lab on a chip 2011;11(19):3287-93

Organic bioelectronics in nanomedicine
Svennersten K, Larsson Kc, Berggren M, Richter-dahlfors A
Biochimica et biophysica acta 2011;1810(3):276-85

Special issue on "nanotechnologies: emerging applications in biomedicine"
Richter-dahlfors A, Kjall P
Biochimica et biophysica acta 2011;1810(3):237-8

Uropathogenic Escherichia coli P and Type 1 fimbriae act in synergy in a living host to facilitate renal colonization leading to nephron obstruction
Melican K, Sandoval Rm, Kader A, Josefsson L, Tanner Ga, Molitoris Ba, et al
PLoS pathogens 2011;7(2):e1001298-

Ion bipolar junction transistors
Tybrandt K, Larsson Kc, Richter-dahlfors A, Berggren M
Proceedings of the National Academy of Sciences of the United States of America 2010;107(22):9929-32

Novel innate immune functions revealed by dynamic, real-time live imaging of bacterial infections
Melican K, Boekel J, Ryden-aulin M, Richter-dahlfors A
Critical reviews in immunology 2010;30(2):107-17

Active control of epithelial cell-density gradients grown along the channel of an organic electrochemical transistor
Bolin Mh, Svennersten K, Nilsson D, Sawatdee A, Jager Ew, Richter-dahlfors A, et al
Advanced materials (Deerfield Beach, Fla.) 2009;21(43):4379-82

Electrochemical modulation of epithelia formation using conducting polymers
Svennersten K, Bolin Mh, Jager Ew, Berggren M, Richter-dahlfors A
Biomaterials 2009;30(31):6257-64

Multiphoton imaging of host-pathogen interactions
Melican K, Richter-dahlfors A
Biotechnology journal 2009;4(6):804-11

Nano-fiber scaffold electrodes based on PEDOT for cell stimulation
Bolin Mh, Svennersten K, Wang Xj, Chronakis Is, Richter-dahlfors A, Jager Ewh, et al

Omeprazole antagonizes virulence and inflammation in Salmonella enterica-infected RAW264.7 cells
Puiac S, Negrea A, Richter-dahlfors A, Plant L, Rhen M
Antimicrobial agents and chemotherapy 2009;53(6):2402-9

Organic electronics for precise delivery of neurotransmitters to modulate mammalian sensory function
Simon Dt, Kurup S, Larsson Kc, Hori R, Tybrandt K, Goiny M, et al
Nature materials 2009;8(9):742-6

Real-time live imaging to study bacterial infections in vivo
Melican K, Richter-dahlfors A
Current opinion in microbiology 2009;12(1):31-6

Role of alpha-hemolysin induced Ca2+ signaling in the progression of uropathogenic E-coli infection within the urinary tract
Richter-dahlfors A, Melican K, Boekel J, Mansson L, Sandoval Rm, Tanner Ga, et al
FEBS JOURNAL 2009;:51-51

Translating Electronic Currents to Precise Acetylcholine-Induced Neuronal Signaling Using an Organic Electrophoretic Delivery Device
Tybrandt K, Larsson Kc, Kurup S, Simon Dt, Kjall P, Isaksson J, et al
ADVANCED MATERIALS 2009;21(44):4442-+

A bacterial cytotoxin identifies the RhoA exchange factor Net1 as a key effector in the response to DNA damage
Guerra L, Carr Hs, Richter-dahlfors A, Masucci Mg, Thelestam M, Frost Ja, et al
PloS one 2008;3(5):e2254-

Bacterial infection-mediated mucosal signalling induces local renal ischaemia as a defence against sepsis
Melican K, Boekel J, Mansson Le, Sandoval Rm, Tanner Ga, Kallskog O, et al
Cellular microbiology 2008;10(10):1987-98

Electronically controlled pH gradients and proton oscillations
Isaksson J, Nilsson D, Kjall P, Robinson Nd, Richter-dahlfors A, Berggren M
ORGANIC ELECTRONICS 2008;9(3):303-309

Electronic control of Ca2+ signalling in neuronal cells using an organic electronic ion pump
Isaksson J, Kjall P, Nilsson D, Robinson Nd, Berggren M, Richter-dahlfors A
Nature materials 2007;6(9):673-9

Organic bioelectronics
Berggren M, Richter-dahlfors A
ADVANCED MATERIALS 2007;19(20):3201-3213

Progression of bacterial infections studied in real time--novel perspectives provided by multiphoton microscopy
Mansson Le, Melican K, Molitoris Ba, Richter-dahlfors A
Cellular microbiology 2007;9(10):2334-43

Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals
Mansson Le, Melican K, Boekel J, Sandoval Rm, Hautefort I, Tanner Ga, et al
Cellular microbiology 2007;9(2):413-24

Role of the lipopolysaccharide-CD14 complex for the activity of hemolysin from uropathogenic Escherichia coli
Mansson Le, Kjall P, Pellett S, Nagy G, Welch Ra, Backhed F, et al
Infection and immunity 2007;75(2):997-1004

A minimal generic model of bacteria-induced intracellular Ca2+ oscillations in epithelial cells
Oxhamre C, Richter-dahlfors A, Zhdanov Vp, Kasemo B
Biophysical journal 2005;88(4):2976-81

Effects of the Escherichia coli toxin cytolysin A on mucosal immunostimulation via epithelial Ca2+ signalling and Toll-like receptor 4
Soderblom T, Oxhamre C, Wai Sn, Uhlen P, Aperia A, Uhlin Be, et al
Cellular microbiology 2005;7(6):779-88

Imaging Techniques for the Study of Escherichia coli and Salmonella Infections
Torstensson E, Kjall P, Richter-dahlfors A
EcoSal Plus 2005;1(2):-

Bacterial protein toxins and inflammation
Soderblom T, Oxhamre C, Torstensson E, Richter-dahlfors A
Scandinavian journal of infectious diseases 2003;35(9):628-31

Developmental aspects of Escherichia coli-induced innate responses in rat renal epithelial cells
Laestadius A, Soderblom T, Aperia A, Richter-dahlfors A
Pediatric research 2003;54(4):536-41

Gastric mucosal recognition of Helicobacter pylori is independent of Toll-like receptor 4
Backhed F, Rokbi B, Torstensson E, Zhao Y, Nilsson C, Seguin D, et al
The Journal of infectious diseases 2003;187(5):829-36

Helicobacter pylori infection induces interleukin-8 receptor expression in the human gastric epithelium
Backhed F, Torstensson E, Seguin D, Richter-dahlfors A, Rokbi B
Infection and immunity 2003;71(6):3357-60

Nanoscale features influence epithelial cell morphology and cytokine production
Andersson As, Backhed F, Von Euler A, Richter-dahlfors A, Sutherland D, Kasemo B
Biomaterials 2003;24(20):3427-36

Structural requirements for TLR4-mediated LPS signalling: a biological role for LPS modifications
Backhed F, Normark S, Schweda Ek, Oscarson S, Richter-dahlfors A
Microbes and infection / Institut Pasteur 2003;5(12):1057-63

Vesicle-mediated export and assembly of pore-forming oligomers of the enterobacterial ClyA cytotoxin
Wai Sn, Lindmark B, Soderblom T, Takade A, Westermark M, Oscarsson J, et al
Cell 2003;115(1):25-35

Dual effects of Escherichia coli alpha-hemolysin on rat renal proximal tubule cells
Laestadius A, Richter-dahlfors A, Aperia A
Kidney international 2002;62(6):2035-42

Expression and regulation of the pattern recognition receptors Toll-like receptor-2 and Toll-like receptor-4 in the human placenta
Holmlund U, Cebers G, Dahlfors Ar, Sandstedt B, Bremme K, Ekstrom Es, et al
Immunology 2002;107(1):145-51

Host-pathogen interactions: Structure and function of pili
Barnhart Mm, Schilling Jd, Backhed F, Dahlfors Ar, Normark S, Hultgren Sj

Identification of target tissue glycosphingolipid receptors for uropathogenic, F1C-fimbriated Escherichia coli and its role in mucosal inflammation
Backhed F, Alsen B, Roche N, Angstrom J, Von Euler A, Breimer Me, et al
The Journal of biological chemistry 2002;277(20):18198-205

TLR4-dependent lipopolysaccharide signalling in epithelial cells is independent of extracellular protease activity
Backhed F, Normark S, Richter-dahlfors A
Cellular microbiology 2002;4(5):297-303

TLR4-dependent recognition of lipopolysaccharide by epithelial cells requires sCD14
Backhed F, Meijer L, Normark S, Richter-dahlfors A
Cellular microbiology 2002;4(8):493-501

Toll-like receptor 4 resides in the Golgi apparatus and colocalizes with internalized lipopolysaccharide in intestinal epithelial cells
Hornef Mw, Frisan T, Vandewalle A, Normark S, Richter-dahlfors A
The Journal of experimental medicine 2002;195(5):559-70

Toxin-induced calcium oscillations: a novel strategy to affect gene regulation in target cells
Soderblom T, Laestadius A, Oxhamre C, Aperia A, Richter-dahlfors A
International journal of medical microbiology : IJMM 2002;291(6-7):511-5

Induction of innate immune responses by Escherichia coli and purified lipopolysaccharide correlate with organ- and cell-specific expression of Toll-like receptors within the human urinary tract
Backhed F, Soderhall M, Ekman P, Normark S, Richter-dahlfors A
Cellular microbiology 2001;3(3):153-8

Receptor-mediated endocytosis in an apicomplexan parasite (Toxoplasma gondii)
Botero-kleiven S, Fernandez V, Lindh J, Richter-dahlfors A, Von Euler A, Wahlgren M
Experimental parasitology 2001;98(3):134-44

Uroepithelial cells require TLR4, CD14 and proteases for induction of proinflammatory responses when exposed to LPS
Backhed F, Normark S, Richter-dahlfors A

Alpha-haemolysin of uropathogenic E. coli induces Ca2+ oscillations in renal epithelial cells
Uhlen P, Laestadius A, Jahnukainen T, Soderblom T, Backhed F, Celsi G, et al
Nature 2000;405(6787):694-7

Helicobacter pylori-infected human antral primary cell cultures: effect on gastrin cell function
Richter-dahlfors A, Heczko U, Meloche Rm, Finlay Bb, Buchan Am
The American journal of physiology 1998;275(3 Pt 1):G393-401

Murine salmonellosis studied by confocal microscopy: Salmonella typhimurium resides intracellularly inside macrophages and exerts a cytotoxic effect on phagocytes in vivo
Richter-dahlfors A, Buchan Am, Finlay Bb
The Journal of experimental medicine 1997;186(4):569-80

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